FastQCFastQC Report
Thu 2 Feb 2017
SRR4062853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433094
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27410.19126449486216535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19900.13886039575910583No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19050.13292917282467168No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18280.12755618263700774No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17330.12092716876911075No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16920.1180662259419131No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16740.11681020226167999No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15840.1105300838605144No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15830.1104603047671681No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15670.10934383927362755No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15090.10529665185954305No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14340.10006321985857174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT502.7955673E-522.402767
ACGATTA507.199605E-419.19901732
ACCGTCG3250.018.217638
TACCGTC3250.018.217637
TCTAGCG5000.016.95913128
CATCGTT3350.016.7155628
ATACCGT3450.016.697716
CCGTCGT3550.016.677539
CGTCGTA3400.016.47089610
CTAGCGG5150.016.46517829
TAGCGGC5250.016.15155430
TAGACCG600.002440292416.0019725
TAGATCG600.002440292416.0019725
ATCGTTT3600.015.99918129
ATCGCCA3550.015.77383916
CGCATCG3600.015.55475913
AGAACCG2800.015.4304715
CGGTCCA5500.015.418467510
CGCCGGT5400.015.4093067
GTATTAG4100.015.2213871
Produced by FastQC (version 0.11.5)