FastQCFastQC Report
Thu 2 Feb 2017
SRR4062852_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062852_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030425
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46770.23034586355073447No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT35410.17439698585271557No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC33610.16553184678084637No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT30270.14908208872526688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27830.13706490020562198No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC26200.1290370242683182No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25770.1269192410455939No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25400.12509696245859858No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25360.1248999593681126No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24860.12243742073703781No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24590.12110764987625744No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22800.11229176157700974No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22200.10933671521972002No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG21930.10800694435893962No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21860.10766218895058917No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21080.10382062868611253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6150.018.21073728
CTAGCGG6150.018.21073729
GTATCAA41200.017.1044671
CATCGTT4700.017.02067428
TAGCGGC6600.016.96909530
ATCGTTT4800.016.66607729
GCATCGT5300.015.9990427
CAAGACG8100.015.9970714
AAGACGG8000.015.7971065
AGCGGCG7200.015.7772231
GTCCTAC6300.015.5075581
AACGAAT1451.2223609E-915.44772931
CGCTTCG5650.015.29149332
TCGTTTA5150.015.22276230
TCCTACA6650.015.1625862
TAGGACG4250.015.0560644
GTATTAC1603.0013325E-1015.0151
GTCTAGT756.200075E-414.9482691
TCCTATA1501.9663275E-914.9379562
GTAATAC1501.9754225E-914.9324373
Produced by FastQC (version 0.11.5)