##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062852_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2030425
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229635667409532	32.0	32.0	32.0	32.0	32.0
2	30.77279091815753	32.0	32.0	32.0	32.0	32.0
3	30.826259034438603	32.0	32.0	32.0	32.0	32.0
4	30.90730709087999	32.0	32.0	32.0	32.0	32.0
5	30.777601733627197	32.0	32.0	32.0	32.0	32.0
6	34.457719935481485	36.0	36.0	36.0	32.0	36.0
7	34.386738736963935	36.0	36.0	36.0	32.0	36.0
8	34.35542805077755	36.0	36.0	36.0	32.0	36.0
9	34.53591341714173	36.0	36.0	36.0	32.0	36.0
10	34.19431990839356	36.0	36.0	36.0	32.0	36.0
11	34.514829653890196	36.0	36.0	36.0	32.0	36.0
12	34.297546080254136	36.0	36.0	36.0	32.0	36.0
13	34.41046381915116	36.0	36.0	36.0	32.0	36.0
14	34.29446790696529	36.0	36.0	36.0	32.0	36.0
15	34.217424923353484	36.0	36.0	36.0	32.0	36.0
16	34.244522205942104	36.0	36.0	36.0	32.0	36.0
17	34.15409286233178	36.0	36.0	36.0	32.0	36.0
18	34.18734698400581	36.0	36.0	36.0	32.0	36.0
19	34.17657928758757	36.0	36.0	36.0	32.0	36.0
20	34.16010933671522	36.0	36.0	36.0	32.0	36.0
21	34.148224632773925	36.0	36.0	36.0	32.0	36.0
22	34.12130268293584	36.0	36.0	36.0	32.0	36.0
23	34.0635610771144	36.0	36.0	36.0	32.0	36.0
24	34.04456948668382	36.0	36.0	36.0	32.0	36.0
25	34.026862356403214	36.0	36.0	36.0	32.0	36.0
26	33.97312237585727	36.0	36.0	36.0	32.0	36.0
27	33.98480367410764	36.0	36.0	36.0	32.0	36.0
28	33.965249639853724	36.0	36.0	36.0	32.0	36.0
29	33.927010354974946	36.0	36.0	36.0	32.0	36.0
30	33.89880394498689	36.0	36.0	36.0	32.0	36.0
31	33.924640407796396	36.0	36.0	36.0	32.0	36.0
32	33.87430217811542	36.0	36.0	36.0	32.0	36.0
33	33.841324353275795	36.0	36.0	36.0	32.0	36.0
34	33.84483002327099	36.0	36.0	36.0	32.0	36.0
35	33.821383207949076	36.0	36.0	36.0	32.0	36.0
36	33.76611940849822	36.0	36.0	36.0	32.0	36.0
37	33.77048844453747	36.0	36.0	36.0	32.0	36.0
38	33.168862184025514	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	2.0
8	5.0
9	8.0
10	20.0
11	29.0
12	10.0
13	9.0
14	135.0
15	383.0
16	528.0
17	649.0
18	803.0
19	1148.0
20	1608.0
21	2530.0
22	3854.0
23	6208.0
24	9419.0
25	14289.0
26	21120.0
27	30178.0
28	42977.0
29	57129.0
30	76301.0
31	103101.0
32	140072.0
33	209848.0
34	455131.0
35	852927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13769121451233	16.742999608473152	11.093918118619813	26.025391058394696
2	17.414355286415358	18.777850762537433	37.06288192779634	26.744912023250876
3	19.75313628435579	22.529922090997438	27.571373896716022	30.14556772793075
4	12.81720913097356	14.568471466200151	35.25377715522931	37.36054224759698
5	15.250944999199675	35.74832855190416	33.0743563539653	15.926370094930864
6	35.59841589346829	34.80926843178674	16.12386408913628	13.468451585608685
7	31.603873480488293	29.51174681913415	19.853015777015813	19.031363923361745
8	28.868503366549824	32.560060322583986	18.513438372511658	20.057997938354536
9	27.421545711277183	14.157183397704316	18.21418052834441	40.207090362674094
10	16.501446009093705	25.744477978896935	30.4779414661823	27.276134545827063
11	38.72965776883365	20.792639020637495	21.37282540920871	19.104877801320146
12	25.606841782787974	23.154355805014706	27.732930512404767	23.50587189979255
13	29.77284426175001	18.69523990138441	25.05131507976188	26.480600757103694
14	23.88074551783483	19.47652783230134	24.12589220933961	32.51683444052422
15	25.5787259973605	27.05916894794065	21.571204566850234	25.79090048784861
16	26.52684662106482	25.356409108350437	23.219358273966705	24.897385996618034
17	24.52466678522185	25.65929900389015	24.402991728559204	25.41304248232879
18	25.592758103863623	24.203010692524295	25.7837646413308	24.42046656228128
19	26.038995101600538	24.25365286626557	24.85293719261866	24.854414839515233
20	26.365764136390236	23.679352526631636	24.383192171372396	25.571691165605735
21	27.604952427673663	23.602395141155615	23.845120641173594	24.94753178999713
22	26.69166840289835	23.73761180223804	24.57794793669182	24.99277185817179
23	24.756207966253804	23.74675773095841	25.365601852809604	26.13143244997818
24	25.263900916104383	24.492221634407457	24.930028697839237	25.31384875164892
25	25.23969640425838	24.12490616077885	24.98965551892843	25.645741916034343
26	24.951823039818404	25.338815063526592	25.317386660991836	24.391975235663168
27	25.73976767187198	24.250529003581857	24.675554320885496	25.334149003660666
28	24.830541783603756	24.240134680002008	25.432228041850095	25.49709549454414
29	24.76025536639344	24.481955057209184	25.272524962060032	25.485264614337343
30	24.455894035951456	24.846861303155272	25.636479245686335	25.060765415206944
31	25.691240551268557	24.559732334991054	24.125559831335188	25.623467282405205
32	25.2512580758446	24.50584475169225	24.1651040766238	26.077793095839347
33	24.665703229975687	24.21102612903353	24.97896792090756	26.144302720083225
34	25.781296948501343	24.33376844075561	25.029874639804937	24.855059970938107
35	26.117720078617978	24.1193653420818	25.148787972828522	24.614126606471697
36	24.56960205861983	24.997512435360264	24.745701931630887	25.68718357438902
37	25.989378459610663	24.643067184050548	24.477124086426358	24.890430269912432
38	24.909483597211242	24.376703878007877	24.97888237916165	25.734930145619234
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	65.0
2	106.0
3	106.0
4	321.0
5	536.0
6	536.0
7	711.5
8	887.0
9	998.5
10	1110.0
11	1110.0
12	1512.0
13	1914.0
14	2630.0
15	3346.0
16	3346.0
17	4966.0
18	6586.0
19	6586.0
20	8265.5
21	9945.0
22	10536.5
23	11128.0
24	11128.0
25	13809.5
26	16491.0
27	16491.0
28	21754.5
29	27018.0
30	34361.0
31	41704.0
32	41704.0
33	53983.5
34	66263.0
35	66263.0
36	74868.0
37	83473.0
38	96191.5
39	108910.0
40	108910.0
41	115660.0
42	122410.0
43	138203.0
44	153996.0
45	153996.0
46	156209.0
47	158422.0
48	158422.0
49	168104.5
50	177787.0
51	185112.5
52	192438.0
53	192438.0
54	184532.5
55	176627.0
56	176627.0
57	171506.0
58	166385.0
59	151175.5
60	135966.0
61	135966.0
62	129928.5
63	123891.0
64	104897.5
65	85904.0
66	85904.0
67	72956.0
68	60008.0
69	60008.0
70	48384.5
71	36761.0
72	29298.0
73	21835.0
74	21835.0
75	16792.5
76	11750.0
77	11750.0
78	10845.0
79	9940.0
80	7858.0
81	5776.0
82	5776.0
83	5517.5
84	5259.0
85	5259.0
86	3609.5
87	1960.0
88	1641.5
89	1323.0
90	1323.0
91	982.5
92	642.0
93	557.0
94	472.0
95	472.0
96	405.5
97	339.0
98	339.0
99	716.0
100	1093.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1215016560572294
2	0.05328933597645813
3	0.011623182338672938
4	0.0030042971299112254
5	0.0
6	2.95504635728973E-4
7	1.477523178644865E-4
8	3.9400618097196394E-4
9	3.447554083504685E-4
10	4.4325695359345946E-4
11	0.0021177832227243067
12	0.0037923094918551536
13	0.01576024723887856
14	0.010441163795757047
15	0.024231380129775784
16	0.012017188519644902
17	0.021079330682000075
18	0.006304098895551423
19	0.008372631345654235
20	0.006550352758658901
21	0.007289114347981333
22	0.008175628255168252
23	0.01039191302313555
24	0.01511998719479912
25	0.017582525825873895
26	0.020143566002191658
27	0.009505399115948632
28	0.006451851213415911
29	0.012017188519644902
30	0.0037430587192336584
31	0.006205597350308433
32	0.006993609712252361
33	0.009505399115948632
34	0.013543962470911263
35	0.0174840242806309
36	0.016105002647229028
37	0.01039191302313555
38	0.0064026004407944145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2030425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.784934650061395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06995593301606	35.394280037921
2	14.42842665797891	13.78922849909452
3	5.055336168041671	7.247067252718891
4	2.3049158945739388	4.405610215844139
5	1.2137441790731838	2.8999343139452254
6	0.6963461730534081	1.9964913823906472
7	0.4461162799164562	1.4922346097495451
8	0.3140371318468431	1.200499505839529
9	0.22718857733863987	0.9770572189230593
>10	1.0624513755596978	9.002404810348438
>50	0.08003513178477974	2.6778991410113213
>100	0.08043424265419316	8.491938226353012
>500	0.014077175872257892	4.7338155040178815
>1k	0.006935079290009403	5.691539281842893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4677	0.23034586355073447	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3541	0.17439698585271557	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3361	0.16553184678084637	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3027	0.14908208872526688	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2783	0.13706490020562198	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2620	0.1290370242683182	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2577	0.1269192410455939	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2540	0.12509696245859858	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2536	0.1248999593681126	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2486	0.12243742073703781	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2459	0.12110764987625744	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2280	0.11229176157700974	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2220	0.10933671521972002	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2193	0.10800694435893962	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2186	0.10766218895058917	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2108	0.10382062868611253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.92507726214955E-5	0.0
10	0.0	0.0	0.0	4.92507726214955E-5	0.0
11	0.0	0.0	0.0	9.8501545242991E-5	0.0
12	0.0	0.0	0.0	9.8501545242991E-5	9.8501545242991E-5
13	0.0	0.0	0.0	9.8501545242991E-5	1.97003090485982E-4
14	0.0	0.0	0.0	9.8501545242991E-5	1.97003090485982E-4
15	0.0	0.0	0.0	9.8501545242991E-5	1.97003090485982E-4
16	0.0	0.0	0.0	9.8501545242991E-5	1.97003090485982E-4
17	0.0	0.0	0.0	9.8501545242991E-5	2.462538631074775E-4
18	0.0	0.0	0.0	9.8501545242991E-5	2.462538631074775E-4
19	0.0	0.0	0.0	9.8501545242991E-5	2.462538631074775E-4
20	0.0	0.0	0.0	9.8501545242991E-5	2.462538631074775E-4
21	0.0	0.0	0.0	1.97003090485982E-4	2.462538631074775E-4
22	0.0	0.0	0.0	2.462538631074775E-4	2.462538631074775E-4
23	0.0	0.0	0.0	5.417584988364505E-4	3.447554083504685E-4
24	0.0	0.0	0.0	9.357646798084145E-4	3.447554083504685E-4
25	0.0	0.0	0.0	0.0010342662250514055	3.94006180971964E-4
26	0.0	0.0	0.0	0.0014282724060233694	3.94006180971964E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	615	0.0	18.210737	28
CTAGCGG	615	0.0	18.210737	29
GTATCAA	4120	0.0	17.104467	1
CATCGTT	470	0.0	17.020674	28
TAGCGGC	660	0.0	16.969095	30
ATCGTTT	480	0.0	16.666077	29
GCATCGT	530	0.0	15.99904	27
CAAGACG	810	0.0	15.997071	4
AAGACGG	800	0.0	15.797106	5
AGCGGCG	720	0.0	15.77722	31
GTCCTAC	630	0.0	15.507558	1
AACGAAT	145	1.2223609E-9	15.447729	31
CGCTTCG	565	0.0	15.291493	32
TCGTTTA	515	0.0	15.222762	30
TCCTACA	665	0.0	15.162586	2
TAGGACG	425	0.0	15.056064	4
GTATTAC	160	3.0013325E-10	15.015	1
GTCTAGT	75	6.200075E-4	14.948269	1
TCCTATA	150	1.9663275E-9	14.937956	2
GTAATAC	150	1.9754225E-9	14.932437	3
>>END_MODULE