FastQCFastQC Report
Thu 2 Feb 2017
SRR4062852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030425
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50400.24822389401233733No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC36020.1774012829826268No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT35340.1740522304443651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32490.16001576024723888No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT29060.14312274523806592No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC27880.13731115406872946No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27520.13553812625435563No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26180.1289385227230752No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25840.12726399645394437No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC25220.12421044855141165No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24650.12140315451198641No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC24640.12135390373936492No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24360.11997488210596302No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23600.11623182338672938No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG22160.10913971212923404No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG21940.10805619513156112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21190.10436238718494896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5300.021.13165329
TCTAGCG5350.020.93416228
TAGCGGC6000.018.93295530
CGTCTAT1006.8137524E-817.6018141
CAAGACG7850.017.324924
GTATCAA35850.016.6488571
TTAGGAC3500.016.4572183
AGCGGCG7100.016.45037531
CGTCGTA5750.016.41746710
ACCGTCG5850.016.4107368
CCGTCGT5950.016.4038419
ATACCGT6000.016.2671436
TACCGTC5950.016.1349267
TAGGACG4450.015.8202964
AAGACGG8200.015.805345
GTTTTCG6450.015.62759528
ATCGTTT5500.014.83606729
TTACACG650.004162911314.7692994
TTCGGAA6750.014.69600231
GTCCTAA4250.014.6838671
Produced by FastQC (version 0.11.5)