##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2030425
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21618971397614	32.0	32.0	32.0	32.0	32.0
2	31.276581011364616	32.0	32.0	32.0	32.0	32.0
3	31.360885528891735	32.0	32.0	32.0	32.0	32.0
4	31.461078838174274	32.0	32.0	32.0	32.0	32.0
5	31.37460630163636	32.0	32.0	32.0	32.0	32.0
6	34.900682861962395	36.0	36.0	36.0	36.0	36.0
7	34.9352120861396	36.0	36.0	36.0	36.0	36.0
8	34.865725156063384	36.0	36.0	36.0	32.0	36.0
9	34.97651082901363	36.0	36.0	36.0	36.0	36.0
10	34.81783813733578	36.0	36.0	36.0	32.0	36.0
11	35.00465863058227	36.0	36.0	36.0	36.0	36.0
12	34.895376091212434	36.0	36.0	36.0	32.0	36.0
13	34.96038563354963	36.0	36.0	36.0	36.0	36.0
14	34.8913700333674	36.0	36.0	36.0	32.0	36.0
15	34.863413817304256	36.0	36.0	36.0	32.0	36.0
16	34.880134947116986	36.0	36.0	36.0	32.0	36.0
17	34.839341024662325	36.0	36.0	36.0	32.0	36.0
18	34.84336185773914	36.0	36.0	36.0	32.0	36.0
19	34.83646034697169	36.0	36.0	36.0	32.0	36.0
20	34.825328194836054	36.0	36.0	36.0	32.0	36.0
21	34.81275890515533	36.0	36.0	36.0	32.0	36.0
22	34.80002758043267	36.0	36.0	36.0	32.0	36.0
23	34.74238940123373	36.0	36.0	36.0	32.0	36.0
24	34.72337220040139	36.0	36.0	36.0	32.0	36.0
25	34.70445990371474	36.0	36.0	36.0	32.0	36.0
26	34.642816651686225	36.0	36.0	36.0	32.0	36.0
27	34.62906534346258	36.0	36.0	36.0	32.0	36.0
28	34.59280002955046	36.0	36.0	36.0	32.0	36.0
29	34.56046443478582	36.0	36.0	36.0	32.0	36.0
30	34.53762488149033	36.0	36.0	36.0	32.0	36.0
31	34.542503170518486	36.0	36.0	36.0	32.0	36.0
32	34.5105049435463	36.0	36.0	36.0	32.0	36.0
33	34.48024329881675	36.0	36.0	36.0	32.0	36.0
34	34.477954615413026	36.0	36.0	36.0	32.0	36.0
35	34.44742701651132	36.0	36.0	36.0	32.0	36.0
36	34.425821687577724	36.0	36.0	36.0	32.0	36.0
37	34.42281739044781	36.0	36.0	36.0	32.0	36.0
38	33.98841720329488	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	39.0
22	120.0
23	432.0
24	1273.0
25	3190.0
26	6857.0
27	13334.0
28	23780.0
29	37000.0
30	56888.0
31	82228.0
32	118825.0
33	191514.0
34	469095.0
35	1025839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3947812757976	18.002066762944853	11.811136483193021	26.792015478064535
2	16.332590467512958	19.787088909957276	36.35987539554527	27.520445226984496
3	18.660477486240566	23.32659418594629	27.881207136436952	30.131721191376187
4	12.234150223869609	15.44702754056667	35.23132864432682	37.0874935912369
5	14.628823649062289	36.34168840679996	32.77573195339099	16.253755990746754
6	35.55687985736941	35.222811044020446	15.7577250027088	13.46258409590134
7	31.30143945516718	29.832039759242047	19.74442738722659	19.122093398364182
8	28.662453635229497	32.76097375499109	18.383376010346897	20.193196599432518
9	27.250254636151045	14.012967235173477	18.060992499807917	40.67578562886756
10	16.214729362142663	26.003554952282965	30.39139443471186	27.39032125086251
11	38.733646155581376	20.769597893547513	21.32930903452783	19.16744691634328
12	25.425970981373315	23.28931945114804	27.568237113503606	23.71647245397504
13	29.743767551770027	18.74565976924994	25.013273102832812	26.497299576147228
14	23.758080204883214	19.437309922799415	24.114705049435464	32.68990482288191
15	25.513525493431178	27.19750791090535	21.406405062979424	25.882561532684043
16	26.49889875218181	25.43695306990067	23.07783989944957	24.986308278467945
17	24.553480182720367	25.735744979499366	24.297967174360046	25.412807663420217
18	25.65521996626322	24.217195907260795	25.6492113720034	24.478372754472584
19	25.998054594481452	24.32968467192829	24.781708263048376	24.890552470541884
20	26.36329832424246	23.746604774862405	24.340618343450263	25.54947855744487
21	27.507237411730173	23.67054730494449	23.90010549531083	24.922109788014513
22	26.459744368663884	23.83004732016564	24.681445845793785	25.02876246537669
23	24.621691757522207	23.673858771161253	25.427534486690202	26.276914984626345
24	25.200832338057307	24.49290173239593	24.884346873191575	25.421919056355197
25	25.182030945260692	24.12698037454246	25.028713214579813	25.662275465617036
26	24.972074798170237	25.283832342407297	25.264772284015557	24.47932057540691
27	25.778002142408607	24.17557900439563	24.637403499267393	25.409015353928368
28	24.83017102330793	24.221776229114596	25.487422583941786	25.460630163635695
29	24.671928290875066	24.40410258935937	25.364246401615425	25.55972271815014
30	24.529298053363213	24.678872649814693	25.689941760961375	25.10188753586072
31	25.641429749929202	24.376916162872305	24.3155004494133	25.666153637785193
32	25.341000036938077	24.413263233066967	24.24270780747873	26.00302892251622
33	24.601844441434675	24.113178275484195	25.132275262568182	26.152702020512947
34	25.595084772892374	24.25915756553431	25.11247645197434	25.03328120959897
35	25.998842606843397	24.143073494465444	25.186155607816097	24.671928290875066
36	24.54865360700346	24.872674440080278	24.88749892263935	25.691173030276914
37	26.061046332664343	24.54244800965315	24.390361623797972	25.00614403388453
38	24.922984028451214	24.21234393030418	25.033404369434347	25.83126767181026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	33.0
2	52.0
3	52.0
4	230.0
5	408.0
6	408.0
7	569.0
8	730.0
9	914.0
10	1098.0
11	1098.0
12	1501.0
13	1904.0
14	2650.5
15	3397.0
16	3397.0
17	5179.5
18	6962.0
19	6962.0
20	8532.5
21	10103.0
22	10816.5
23	11530.0
24	11530.0
25	13842.5
26	16155.0
27	16155.0
28	21507.0
29	26859.0
30	34385.5
31	41912.0
32	41912.0
33	54718.5
34	67525.0
35	67525.0
36	75518.0
37	83511.0
38	95857.0
39	108203.0
40	108203.0
41	115356.0
42	122509.0
43	138742.0
44	154975.0
45	154975.0
46	156914.5
47	158854.0
48	158854.0
49	169036.5
50	179219.0
51	184902.0
52	190585.0
53	190585.0
54	184248.5
55	177912.0
56	177912.0
57	172936.5
58	167961.0
59	151900.0
60	135839.0
61	135839.0
62	130245.5
63	124652.0
64	105290.0
65	85928.0
66	85928.0
67	73053.5
68	60179.0
69	60179.0
70	48323.0
71	36467.0
72	28992.5
73	21518.0
74	21518.0
75	16477.0
76	11436.0
77	11436.0
78	10504.5
79	9573.0
80	7477.0
81	5381.0
82	5381.0
83	4948.0
84	4515.0
85	4515.0
86	2933.5
87	1352.0
88	1126.0
89	900.0
90	900.0
91	543.5
92	187.0
93	127.5
94	68.0
95	68.0
96	46.5
97	25.0
98	25.0
99	26.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009702402206434613
2	0.0
3	0.0
4	9.850154524299099E-5
5	9.850154524299099E-5
6	2.462538631074775E-4
7	1.9700309048598197E-4
8	0.0028565448120467392
9	0.0037923094918551536
10	8.865139071869189E-4
11	0.004531071081177586
12	2.462538631074775E-4
13	1.477523178644865E-4
14	0.0
15	0.0
16	4.925077262149549E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.477523178644865E-4
22	4.925077262149549E-5
23	1.9700309048598197E-4
24	0.0
25	4.925077262149549E-5
26	4.925077262149549E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.850154524299099E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2030425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.335689506160726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.41713646952908	32.37745124401678
2	15.78030262505571	14.308218002455572
3	5.712769918834266	7.769770897812159
4	2.581146877439061	4.680722936214942
5	1.309600685545743	2.968582502847852
6	0.767683545173946	2.0882077705796838
7	0.5056647266111156	1.6047261327901432
8	0.3430738310464687	1.24427909456094
9	0.23955157643774527	0.9774212301083653
>10	1.1449229785950124	9.205210732078891
>50	0.08694341161274181	2.756076095636191
>100	0.08717285140016458	8.64949799895822
>500	0.015947515440776945	5.06316965771744
>1k	0.007973757720388473	6.057085039399154
>5k	1.0922955781354071E-4	0.24958066482366767
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5040	0.24822389401233733	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3602	0.1774012829826268	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3534	0.1740522304443651	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3249	0.16001576024723888	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2906	0.14312274523806592	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2788	0.13731115406872946	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2752	0.13553812625435563	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2618	0.1289385227230752	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2584	0.12726399645394437	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2522	0.12421044855141165	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2465	0.12140315451198641	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2464	0.12135390373936492	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2436	0.11997488210596302	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2360	0.11623182338672938	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2216	0.10913971212923404	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2194	0.10805619513156112	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2119	0.10436238718494896	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.97003090485982E-4
2	0.0	0.0	0.0	0.0	1.97003090485982E-4
3	0.0	0.0	0.0	0.0	2.9550463572897297E-4
4	0.0	0.0	0.0	0.0	2.9550463572897297E-4
5	0.0	0.0	0.0	0.0	2.9550463572897297E-4
6	0.0	0.0	0.0	0.0	2.9550463572897297E-4
7	0.0	0.0	0.0	0.0	2.9550463572897297E-4
8	0.0	0.0	0.0	0.0	2.9550463572897297E-4
9	0.0	0.0	0.0	4.92507726214955E-5	2.9550463572897297E-4
10	0.0	0.0	0.0	4.92507726214955E-5	2.9550463572897297E-4
11	0.0	0.0	0.0	4.92507726214955E-5	3.447554083504685E-4
12	0.0	0.0	0.0	4.92507726214955E-5	6.89510816700937E-4
13	0.0	0.0	0.0	4.92507726214955E-5	7.88012361943928E-4
14	0.0	0.0	0.0	4.92507726214955E-5	7.88012361943928E-4
15	0.0	0.0	0.0	4.92507726214955E-5	8.372631345654235E-4
16	0.0	0.0	0.0	4.92507726214955E-5	8.372631345654235E-4
17	0.0	0.0	0.0	4.92507726214955E-5	8.86513907186919E-4
18	0.0	0.0	0.0	4.92507726214955E-5	9.357646798084145E-4
19	0.0	0.0	0.0	4.92507726214955E-5	9.357646798084145E-4
20	0.0	0.0	0.0	4.92507726214955E-5	9.357646798084145E-4
21	0.0	0.0	0.0	1.4775231786448649E-4	9.357646798084145E-4
22	0.0	0.0	0.0	2.9550463572897297E-4	9.357646798084145E-4
23	0.0	0.0	0.0	6.402600440794415E-4	9.357646798084145E-4
24	0.0	0.0	0.0	9.357646798084145E-4	0.0010342662250514055
25	0.0	0.0	0.0	0.0010342662250514055	0.0010342662250514055
26	0.0	0.0	0.0	0.0014282724060233694	0.0010342662250514055
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	530	0.0	21.131653	29
TCTAGCG	535	0.0	20.934162	28
TAGCGGC	600	0.0	18.932955	30
CGTCTAT	100	6.8137524E-8	17.601814	1
CAAGACG	785	0.0	17.32492	4
GTATCAA	3585	0.0	16.648857	1
TTAGGAC	350	0.0	16.457218	3
AGCGGCG	710	0.0	16.450375	31
CGTCGTA	575	0.0	16.417467	10
ACCGTCG	585	0.0	16.410736	8
CCGTCGT	595	0.0	16.403841	9
ATACCGT	600	0.0	16.267143	6
TACCGTC	595	0.0	16.134926	7
TAGGACG	445	0.0	15.820296	4
AAGACGG	820	0.0	15.80534	5
GTTTTCG	645	0.0	15.627595	28
ATCGTTT	550	0.0	14.836067	29
TTACACG	65	0.0041629113	14.769299	4
TTCGGAA	675	0.0	14.696002	31
GTCCTAA	425	0.0	14.683867	1
>>END_MODULE