FastQCFastQC Report
Thu 2 Feb 2017
SRR4062851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757037
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38190.5044667565786085No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25250.33353719831395295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24900.32891391041653184No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19620.25916831013543595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14130.18664873711588734No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.1569276006324658No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10870.14358611269990768No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10530.13909491874241287No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10450.1380381672230023No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10300.13605675812410756No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9970.13169765810653905No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9830.12984834294757058No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9750.12879159142816005No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9350.12350783383110733No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9000.11888454593368619No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8840.1167710428948651No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8780.1159784792553072No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8620.11386497621648613No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8200.10831703073958077No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8020.10593933982090703No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7610.100523488283928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2500.021.75905828
CTAGCGG2600.020.9221729
GCCTTAT400.00445415320.0216161
TATAATA400.004478074620.0030982
TTTAGAC400.00448321419.9991323
ACACCGT400.004486643219.996496
TCTTAGG507.185342E-419.2029722
TAAATAC751.5078022E-619.1991653
CGTTTAT2650.018.11242131
GTCTTAG450.00879266617.7969931
CGGTCCA3100.017.54762610
GTAGACT550.001365667417.4514834
TAGCGGC3050.017.31072430
ATCGTTT2900.017.10270729
GGTCCAA3300.016.96896211
GCGGCGC3350.016.71569332
AGCGGCG3200.016.49928531
GATATAC805.565123E-516.0172941
GCAATTA600.002440502416.00036213
GTTTTCG2500.015.99930728
Produced by FastQC (version 0.11.5)