##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062851_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757037
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183529999194228	32.0	32.0	32.0	32.0	32.0
2	30.84978541339459	32.0	32.0	32.0	32.0	32.0
3	30.916227344238127	32.0	32.0	32.0	32.0	32.0
4	30.964585614705754	32.0	32.0	32.0	32.0	32.0
5	30.918096473488085	32.0	32.0	32.0	32.0	32.0
6	34.566552229283374	36.0	36.0	36.0	32.0	36.0
7	34.45109023733318	36.0	36.0	36.0	32.0	36.0
8	34.44846024698925	36.0	36.0	36.0	32.0	36.0
9	34.53181812777975	36.0	36.0	36.0	32.0	36.0
10	34.305373449382266	36.0	36.0	36.0	32.0	36.0
11	34.55667292351629	36.0	36.0	36.0	32.0	36.0
12	34.39069424612007	36.0	36.0	36.0	32.0	36.0
13	34.46349385829226	36.0	36.0	36.0	32.0	36.0
14	34.3601646947243	36.0	36.0	36.0	32.0	36.0
15	34.33317261903976	36.0	36.0	36.0	32.0	36.0
16	34.330204468209615	36.0	36.0	36.0	32.0	36.0
17	34.26324076630336	36.0	36.0	36.0	32.0	36.0
18	34.28563993569667	36.0	36.0	36.0	32.0	36.0
19	34.269052899660124	36.0	36.0	36.0	32.0	36.0
20	34.22253469777567	36.0	36.0	36.0	32.0	36.0
21	34.19963885516824	36.0	36.0	36.0	32.0	36.0
22	34.18006913796816	36.0	36.0	36.0	32.0	36.0
23	34.13585597533542	36.0	36.0	36.0	32.0	36.0
24	34.117700984231945	36.0	36.0	36.0	32.0	36.0
25	34.08078072802254	36.0	36.0	36.0	32.0	36.0
26	34.03927284927949	36.0	36.0	36.0	32.0	36.0
27	34.04081174367963	36.0	36.0	36.0	32.0	36.0
28	34.01353566602425	36.0	36.0	36.0	32.0	36.0
29	33.99475719152432	36.0	36.0	36.0	32.0	36.0
30	33.94124461552077	36.0	36.0	36.0	32.0	36.0
31	33.966952738109235	36.0	36.0	36.0	32.0	36.0
32	33.90127431023847	36.0	36.0	36.0	32.0	36.0
33	33.85226745852581	36.0	36.0	36.0	32.0	36.0
34	33.837208749374206	36.0	36.0	36.0	32.0	36.0
35	33.78340820858161	36.0	36.0	36.0	32.0	36.0
36	33.752659381245564	36.0	36.0	36.0	27.0	36.0
37	33.73307117089389	36.0	36.0	36.0	27.0	36.0
38	33.10788112073782	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	3.0
9	2.0
10	5.0
11	4.0
12	2.0
13	2.0
14	167.0
15	319.0
16	376.0
17	449.0
18	575.0
19	707.0
20	877.0
21	1213.0
22	1797.0
23	2551.0
24	3564.0
25	5115.0
26	7320.0
27	10577.0
28	14852.0
29	19896.0
30	27143.0
31	35796.0
32	48598.0
33	71981.0
34	159158.0
35	343984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.40291575417103	16.77682737169518	11.472847303773765	25.347409570360025
2	16.565782587319795	20.19827025655394	37.077145923314006	26.158801232812262
3	19.0288909820873	25.094028791900115	27.89342478819577	27.98365543781681
4	12.585202954759211	15.613804728037453	35.28617624990753	36.514816067295804
5	14.769178744471862	36.5777849402142	32.61826386063543	16.03477245467851
6	33.858276037435516	34.878968607792245	17.29074613459087	13.972009220181366
7	30.148724566963043	30.62637625373661	20.902148772120782	18.32275040717957
8	28.872831988164272	31.36058016194867	19.787194695058318	19.97939315482874
9	28.226303935749335	14.401711941984189	18.269432721076303	39.10255140119017
10	16.506347172503073	26.32432004015693	30.577916330924797	26.5914164564152
11	37.394784814139655	21.465218884573723	21.6011466011466	19.538849700140023
12	25.65318531454213	23.836458962168816	27.98839133920988	22.521964384079173
13	30.100709577780744	19.38054956277273	24.93932720241925	25.579413657027278
14	24.684137938323012	19.72757392572552	24.778460611697067	30.809827524254402
15	25.77974499570589	26.084957389178832	23.23102332034089	24.904274294774396
16	25.99120688652973	25.498972199176702	23.756863014494897	24.752957899798666
17	24.043040075256712	25.307712227630418	25.348405815483645	25.30084188162922
18	25.46268041563959	23.778536912716213	26.77852898671338	23.98025368493082
19	25.4525518999716	25.17288981948123	25.74476376678534	23.62979451376183
20	25.990322337751202	23.254130443685014	25.807099330119776	24.948447888444004
21	27.540905211930344	23.70349038433594	24.300192603904392	24.455411799829324
22	26.028882964810446	23.88406126401281	24.90336677296542	25.18368899821133
23	24.212188065577237	23.69473030635296	25.72281596364453	26.370265664425276
24	24.582193862048012	25.35531687210178	25.13931275018826	24.92317651566195
25	25.31093853629664	23.891335998583703	25.189786788778466	25.607938676341192
26	24.526875980995765	24.956796550065796	25.93884928205643	24.577478186882008
27	25.605726944990835	24.508608515646984	24.94666326720679	24.939001272155387
28	24.393359550962554	24.217004823004583	26.01925767209117	25.37037795394169
29	24.121895572057607	24.831063897810008	26.327609872870216	24.719430657262173
30	24.512624601394425	24.840359623467993	26.3511639150663	24.29585186007128
31	24.775924998778073	24.96020480818387	24.748712353088038	25.515157839950014
32	24.241375438411087	25.122226698987443	24.819582692091757	25.81681517050971
33	24.05410439627943	24.42835421232501	25.462329966009715	26.055211425385842
34	24.901809246378534	24.856231298178862	25.711115074410955	24.530844381031645
35	25.97557189380578	24.501958608298157	25.60313905788629	23.919330440009777
36	24.425930843509427	25.709552251820206	24.972619335439234	24.891897569231137
37	25.548073963398764	25.542393413732928	24.97248896586254	23.93704365700577
38	24.234538186564226	25.48765247809098	25.1745720573743	25.103237277970496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	619.0
1	486.5
2	354.0
3	354.0
4	752.0
5	1150.0
6	1150.0
7	1323.5
8	1497.0
9	1400.5
10	1304.0
11	1304.0
12	1644.0
13	1984.0
14	2373.5
15	2763.0
16	2763.0
17	3944.5
18	5126.0
19	5126.0
20	5821.0
21	6516.0
22	5992.5
23	5469.0
24	5469.0
25	5925.5
26	6382.0
27	6382.0
28	8259.5
29	10137.0
30	12590.0
31	15043.0
32	15043.0
33	18719.0
34	22395.0
35	22395.0
36	24855.5
37	27316.0
38	32188.0
39	37060.0
40	37060.0
41	40258.5
42	43457.0
43	50681.0
44	57905.0
45	57905.0
46	63324.5
47	68744.0
48	68744.0
49	71127.0
50	73510.0
51	72152.5
52	70795.0
53	70795.0
54	67573.5
55	64352.0
56	64352.0
57	63253.0
58	62154.0
59	55641.0
60	49128.0
61	49128.0
62	46851.5
63	44575.0
64	36412.5
65	28250.0
66	28250.0
67	23531.5
68	18813.0
69	18813.0
70	15054.5
71	11296.0
72	8865.0
73	6434.0
74	6434.0
75	4859.0
76	3284.0
77	3284.0
78	3291.0
79	3298.0
80	2564.5
81	1831.0
82	1831.0
83	1942.0
84	2053.0
85	2053.0
86	1360.0
87	667.0
88	585.5
89	504.0
90	504.0
91	368.0
92	232.0
93	193.0
94	154.0
95	154.0
96	142.5
97	131.0
98	131.0
99	243.0
100	355.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11478963379597035
2	0.05191291839104298
3	0.011624266713515984
4	0.002773972738452678
5	1.32093939926318E-4
6	2.64187879852636E-4
7	0.0
8	9.246575794842261E-4
9	2.64187879852636E-4
10	9.246575794842261E-4
11	0.002245596978747406
12	0.002113503038821088
13	0.014266145512042345
14	0.008057730335505399
15	0.024701566766221462
16	0.010699609134031758
17	0.0212671243281372
18	0.00462328789742113
19	0.006868884876168536
20	0.004755381837347448
21	0.005680039416831674
22	0.006736790936242218
23	0.008850293975063307
24	0.014134051572116025
25	0.01783268189005293
26	0.020210372808726652
27	0.007133072756021173
28	0.00528375759705272
29	0.012416830353073892
30	0.003038160618305314
31	0.004755381837347448
32	0.005547945476905356
33	0.007397260635873809
34	0.012152642473221256
35	0.016115460671010797
36	0.01532289703145289
37	0.008982387914989624
38	0.005680039416831674
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	757037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.19969630499401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44475596383212	42.264368903002314
2	12.807578687319188	13.627185931353896
3	3.6405504226534617	5.810285306045453
4	1.4370423929561937	3.0580087553068553
5	0.7386983200761695	1.9649263144530742
6	0.4206452866738392	1.3426920901905237
7	0.2702096986842201	1.0062551736065117
8	0.18756874477563454	0.7982880206698153
9	0.12887250627262367	0.6170380376190314
>10	0.6957883809730739	7.129677224199227
>50	0.10342826753589672	3.923904541556063
>100	0.11190166942765249	12.300581974972033
>500	0.010467416739235874	3.821673904810379
>1k	0.0024922420807704463	2.3351138222148347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3819	0.5044667565786085	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2525	0.33353719831395295	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2490	0.32891391041653184	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1962	0.25916831013543595	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1413	0.18664873711588734	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1188	0.1569276006324658	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1087	0.14358611269990768	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1053	0.13909491874241287	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1045	0.1380381672230023	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1030	0.13605675812410756	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	997	0.13169765810653905	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	983	0.12984834294757058	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	975	0.12879159142816005	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	935	0.12350783383110733	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	900	0.11888454593368619	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	884	0.1167710428948651	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	878	0.1159784792553072	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	862	0.11386497621648613	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	820	0.10831703073958077	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	802	0.10593933982090703	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	761	0.100523488283928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.64187879852636E-4
13	0.0	0.0	0.0	0.0	2.64187879852636E-4
14	0.0	0.0	0.0	0.0	2.64187879852636E-4
15	0.0	0.0	0.0	0.0	5.28375759705272E-4
16	0.0	0.0	0.0	2.64187879852636E-4	5.28375759705272E-4
17	0.0	0.0	0.0	2.64187879852636E-4	5.28375759705272E-4
18	0.0	0.0	0.0	2.64187879852636E-4	5.28375759705272E-4
19	0.0	0.0	0.0	2.64187879852636E-4	5.28375759705272E-4
20	0.0	0.0	0.0	2.64187879852636E-4	6.6046969963159E-4
21	0.0	0.0	0.0	5.28375759705272E-4	6.6046969963159E-4
22	0.0	0.0	0.0	0.00132093939926318	6.6046969963159E-4
23	0.0	0.0	0.0	0.00264187879852636	6.6046969963159E-4
24	0.0	0.0	0.0	0.004491193957494812	6.6046969963159E-4
25	0.0	0.0	0.0	0.005547945476905356	6.6046969963159E-4
26	0.0	0.0	0.0	0.007000978816094854	9.24657579484226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	250	0.0	21.759058	28
CTAGCGG	260	0.0	20.92217	29
GCCTTAT	40	0.004454153	20.021616	1
TATAATA	40	0.0044780746	20.003098	2
TTTAGAC	40	0.004483214	19.999132	3
ACACCGT	40	0.0044866432	19.99649	6
TCTTAGG	50	7.185342E-4	19.202972	2
TAAATAC	75	1.5078022E-6	19.199165	3
CGTTTAT	265	0.0	18.112421	31
GTCTTAG	45	0.008792666	17.796993	1
CGGTCCA	310	0.0	17.547626	10
GTAGACT	55	0.0013656674	17.451483	4
TAGCGGC	305	0.0	17.310724	30
ATCGTTT	290	0.0	17.102707	29
GGTCCAA	330	0.0	16.968962	11
GCGGCGC	335	0.0	16.715693	32
AGCGGCG	320	0.0	16.499285	31
GATATAC	80	5.565123E-5	16.017294	1
GCAATTA	60	0.0024405024	16.000362	13
GTTTTCG	250	0.0	15.999307	28
>>END_MODULE