FastQCFastQC Report
Thu 2 Feb 2017
SRR4062851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757037
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46910.6196526721943578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33110.4373630350960389No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25960.34291586804872154No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC24960.32970647405608977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20750.2740949253471099No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.18929061591441368No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11500.1519080309152657No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10630.14041585814167604No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10560.13949120056219183No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10190.13460372478491806No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10010.13222603386624432No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9640.12733855808897057No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9610.1269422762691916No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9610.1269422762691916No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9470.12509296111022317No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9330.12324364595125469No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9180.12126223685235993No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8890.11743151259449672No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8520.11254403681722294No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8470.11188356711759136No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG7890.10422211860186491No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT7880.10409002466193859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCT450.00884524917.7785844
CTTATAT450.00884524917.7785843
GTTAGGA450.00884861617.777412
AAGACGG3000.017.60085
ACCGTCG2200.017.4553388
CAAGACG3350.017.194814
GTTATAT753.2264164E-517.0696961
CGCAAGA3250.016.7381152
TACCGTC2400.016.6674257
TCTAGCG2150.016.37175428
CGTCGTA2250.016.35521910
ATACCGT2350.016.3411676
GCGCAAG3450.016.2347661
GATTATA908.558618E-616.002841
TAATACT1304.78758E-916.0007274
AGAACCG1102.0154584E-716.0007275
CCGTCGT2400.016.0007279
CCTATTC1900.016.0007273
TAACCCG805.6177836E-515.9996728
CTAGCGG2200.015.9996729
Produced by FastQC (version 0.11.5)