##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	757037
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13762735506983	32.0	32.0	32.0	32.0	32.0
2	31.325221884795592	32.0	32.0	32.0	32.0	32.0
3	31.39533866904788	32.0	32.0	32.0	32.0	32.0
4	31.500276736804146	32.0	32.0	32.0	32.0	32.0
5	31.42230168406564	32.0	32.0	32.0	32.0	32.0
6	34.94654158251182	36.0	36.0	36.0	36.0	36.0
7	34.973087180679414	36.0	36.0	36.0	36.0	36.0
8	34.927847648133444	36.0	36.0	36.0	36.0	36.0
9	35.014241047663454	36.0	36.0	36.0	36.0	36.0
10	34.86780170586114	36.0	36.0	36.0	32.0	36.0
11	35.031555921309	36.0	36.0	36.0	36.0	36.0
12	34.9315158968452	36.0	36.0	36.0	32.0	36.0
13	34.979549216220605	36.0	36.0	36.0	36.0	36.0
14	34.93105092617666	36.0	36.0	36.0	32.0	36.0
15	34.89877113007687	36.0	36.0	36.0	32.0	36.0
16	34.90253052360717	36.0	36.0	36.0	32.0	36.0
17	34.86610826155128	36.0	36.0	36.0	32.0	36.0
18	34.85411413180598	36.0	36.0	36.0	32.0	36.0
19	34.84496530552668	36.0	36.0	36.0	32.0	36.0
20	34.82817220294385	36.0	36.0	36.0	32.0	36.0
21	34.81452821988886	36.0	36.0	36.0	32.0	36.0
22	34.78797073326667	36.0	36.0	36.0	32.0	36.0
23	34.719980661447195	36.0	36.0	36.0	32.0	36.0
24	34.70323247080394	36.0	36.0	36.0	32.0	36.0
25	34.67155238119141	36.0	36.0	36.0	32.0	36.0
26	34.61496201638757	36.0	36.0	36.0	32.0	36.0
27	34.59470012694228	36.0	36.0	36.0	32.0	36.0
28	34.55440090774955	36.0	36.0	36.0	32.0	36.0
29	34.51040966293589	36.0	36.0	36.0	32.0	36.0
30	34.47980613893377	36.0	36.0	36.0	32.0	36.0
31	34.463117390563475	36.0	36.0	36.0	32.0	36.0
32	34.43076097997852	36.0	36.0	36.0	32.0	36.0
33	34.3766566231241	36.0	36.0	36.0	32.0	36.0
34	34.35853597644501	36.0	36.0	36.0	32.0	36.0
35	34.29250617869404	36.0	36.0	36.0	32.0	36.0
36	34.26275994436203	36.0	36.0	36.0	32.0	36.0
37	34.228398347769	36.0	36.0	36.0	32.0	36.0
38	33.79898868879592	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	15.0
22	35.0
23	192.0
24	518.0
25	1212.0
26	2668.0
27	5232.0
28	9409.0
29	14765.0
30	22350.0
31	31836.0
32	44481.0
33	70824.0
34	167201.0
35	386296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7977322357809	17.932326406916115	12.262123211754433	26.007818145548555
2	15.670964563158735	21.145597903404987	36.34776107376522	26.83567645967106
3	18.06160068794524	25.644981685175228	28.374967141632446	27.91845048524709
4	12.117257303483587	16.423393339286374	35.286300783582284	36.173048573647755
5	14.172755096514436	36.972565409616706	32.548739361484316	16.305940132384546
6	33.79887640894699	35.39457120325691	16.91964857728222	13.886903810513884
7	29.66796647962041	30.76752369780934	21.08021853765759	18.48429128491266
8	28.68329592784329	31.671067701074872	19.634910088121963	20.010726282959872
9	28.01948193482508	14.043558266810566	18.292781195467953	39.6441786028964
10	16.302082094934654	26.437268997366008	30.693397584759307	26.567251322940034
11	37.47506611607367	21.292281922636928	21.686600563803	19.5460513974864
12	25.322145798135615	24.020881520356443	28.056901086214207	22.600071595293734
13	30.161881551051138	19.276915862542683	25.03054680430892	25.530655782097263
14	24.59258926578225	19.562319939448138	24.936429791410458	30.90866100335915
15	25.591351545565143	26.1984552934665	23.290935581748315	24.919257579220037
16	25.857652928456602	25.357677365835485	23.868978662865885	24.915691042842028
17	24.02458532409909	25.18001101663459	25.418440578201597	25.37696308106473
18	25.435612790392014	23.607828943631553	26.96710992989775	23.989448336078688
19	25.498357411857015	24.97869985218688	26.00678698663341	23.51615574932269
20	26.028450392781334	23.009311301825406	26.13782417504032	24.82441413035294
21	27.5113733032511	23.721593160695132	24.400689002900787	24.36634453315298
22	25.775094216002653	23.85775067797215	25.306821198963853	25.060333907061345
23	24.128971409549877	23.378016369102657	26.008406469441347	26.48460575190612
24	24.57554914753176	25.100094182979166	25.577613775812807	24.746742893676267
25	25.207750744019116	23.572956143491005	25.686062900492313	25.533230211997566
26	24.44213426820618	24.640671459915435	26.282202851379786	24.634991420498604
27	25.674174445898945	24.206214491497775	25.291894583752182	24.8277164788511
28	24.408450313524966	23.84969294763664	26.460001294520612	25.28185544431778
29	24.198156761162267	24.33976146476328	26.76579876545004	24.696283008624416
30	24.567359323256326	24.328401385929617	26.779932817022157	24.3243064737919
31	24.74093076031951	24.41188475596305	25.18463430453201	25.662550179185427
32	24.400524677129386	24.61346010829061	25.21329868949602	25.77271652508398
33	24.081517812207327	23.996977690654486	25.915642168084258	26.00586232905393
34	24.762329978587573	24.295509994887965	26.272559993765164	24.6696000327593
35	26.032941586738822	24.02762348471739	25.935324165133277	24.00411076341051
36	24.53248652311578	25.160196925645646	25.376434705305023	24.930881845933552
37	25.734013000685568	24.95941413695764	25.354771299157107	23.951801563199684
38	24.506364294432498	24.654309702576892	25.547926386591918	25.291399616398692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	281.0
1	234.0
2	187.0
3	187.0
4	669.0
5	1151.0
6	1151.0
7	1338.5
8	1526.0
9	1524.0
10	1522.0
11	1522.0
12	1860.0
13	2198.0
14	2594.5
15	2991.0
16	2991.0
17	4458.5
18	5926.0
19	5926.0
20	6534.0
21	7142.0
22	6506.5
23	5871.0
24	5871.0
25	6008.5
26	6146.0
27	6146.0
28	8068.5
29	9991.0
30	12368.5
31	14746.0
32	14746.0
33	18565.0
34	22384.0
35	22384.0
36	24721.0
37	27058.0
38	31757.5
39	36457.0
40	36457.0
41	39684.0
42	42911.0
43	50127.5
44	57344.0
45	57344.0
46	63480.0
47	69616.0
48	69616.0
49	72294.0
50	74972.0
51	72983.0
52	70994.0
53	70994.0
54	67486.5
55	63979.0
56	63979.0
57	62997.5
58	62016.0
59	55738.5
60	49461.0
61	49461.0
62	47065.0
63	44669.0
64	36411.5
65	28154.0
66	28154.0
67	23433.5
68	18713.0
69	18713.0
70	15085.0
71	11457.0
72	8949.0
73	6441.0
74	6441.0
75	4776.0
76	3111.0
77	3111.0
78	3196.5
79	3282.0
80	2493.0
81	1704.0
82	1704.0
83	1721.5
84	1739.0
85	1739.0
86	1100.5
87	462.0
88	397.5
89	333.0
90	333.0
91	196.0
92	59.0
93	41.5
94	24.0
95	24.0
96	17.0
97	10.0
98	10.0
99	9.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01056751519410544
2	0.0
3	0.0
4	1.32093939926318E-4
5	0.0
6	0.0
7	6.6046969963159E-4
8	0.002377690918673724
9	0.0036986303179369043
10	0.001453033339189498
11	0.00462328789742113
12	5.28375759705272E-4
13	2.64187879852636E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.32093939926318E-4
22	0.0
23	1.32093939926318E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.32093939926318E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	757037.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.9297987406303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86279694825166	39.6553657795702
2	13.743922834071409	13.9995044769202
3	3.9344003159281904	6.01134648765885
4	1.5728651820986126	3.2042282868170866
5	0.7969071143550983	2.0293159474540805
6	0.45388018128580965	1.386961577114825
7	0.27645327129715896	0.9855796627847222
8	0.21262579245127897	0.8663191053288534
9	0.14135240738907714	0.6479144693846354
>10	0.7645742132404069	7.473212124434974
>50	0.10578102913940765	3.8769408155289375
>100	0.11958970177486873	12.823998548877825
>500	0.011985024578685124	4.1344965158877836
>1k	0.002865984138381225	2.9048162022369692
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4691	0.6196526721943578	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3311	0.4373630350960389	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2596	0.34291586804872154	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2496	0.32970647405608977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2075	0.2740949253471099	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1433	0.18929061591441368	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1150	0.1519080309152657	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1063	0.14041585814167604	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1056	0.13949120056219183	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1019	0.13460372478491806	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1001	0.13222603386624432	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	964	0.12733855808897057	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	961	0.1269422762691916	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	961	0.1269422762691916	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	947	0.12509296111022317	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	933	0.12324364595125469	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	918	0.12126223685235993	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	889	0.11743151259449672	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	852	0.11254403681722294	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	847	0.11188356711759136	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	789	0.10422211860186491	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	788	0.10409002466193859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.32093939926318E-4
2	0.0	0.0	0.0	0.0	1.32093939926318E-4
3	0.0	0.0	0.0	0.0	1.32093939926318E-4
4	0.0	0.0	0.0	0.0	1.32093939926318E-4
5	0.0	0.0	0.0	0.0	2.64187879852636E-4
6	0.0	0.0	0.0	0.0	3.96281819778954E-4
7	0.0	0.0	0.0	0.0	3.96281819778954E-4
8	0.0	0.0	0.0	0.0	3.96281819778954E-4
9	0.0	0.0	0.0	0.0	3.96281819778954E-4
10	0.0	0.0	0.0	0.0	3.96281819778954E-4
11	0.0	0.0	0.0	0.0	3.96281819778954E-4
12	0.0	0.0	0.0	0.0	6.6046969963159E-4
13	0.0	0.0	0.0	0.0	6.6046969963159E-4
14	0.0	0.0	0.0	0.0	6.6046969963159E-4
15	0.0	0.0	0.0	0.0	0.001188845459336862
16	0.0	0.0	0.0	2.64187879852636E-4	0.00132093939926318
17	0.0	0.0	0.0	2.64187879852636E-4	0.00132093939926318
18	0.0	0.0	0.0	2.64187879852636E-4	0.00132093939926318
19	0.0	0.0	0.0	2.64187879852636E-4	0.00132093939926318
20	0.0	0.0	0.0	2.64187879852636E-4	0.001453033339189498
21	0.0	0.0	0.0	5.28375759705272E-4	0.001585127279115816
22	0.0	0.0	0.0	0.001453033339189498	0.001585127279115816
23	0.0	0.0	0.0	0.002773972738452678	0.001585127279115816
24	0.0	0.0	0.0	0.004755381837347448	0.001849315158968452
25	0.0	0.0	0.0	0.005812133356757992	0.001849315158968452
26	0.0	0.0	0.0	0.007397260635873808	0.00198140909889477
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGCT	45	0.008845249	17.778584	4
CTTATAT	45	0.008845249	17.778584	3
GTTAGGA	45	0.008848616	17.77741	2
AAGACGG	300	0.0	17.6008	5
ACCGTCG	220	0.0	17.455338	8
CAAGACG	335	0.0	17.19481	4
GTTATAT	75	3.2264164E-5	17.069696	1
CGCAAGA	325	0.0	16.738115	2
TACCGTC	240	0.0	16.667425	7
TCTAGCG	215	0.0	16.371754	28
CGTCGTA	225	0.0	16.355219	10
ATACCGT	235	0.0	16.341167	6
GCGCAAG	345	0.0	16.234766	1
GATTATA	90	8.558618E-6	16.00284	1
TAATACT	130	4.78758E-9	16.000727	4
AGAACCG	110	2.0154584E-7	16.000727	5
CCGTCGT	240	0.0	16.000727	9
CCTATTC	190	0.0	16.000727	3
TAACCCG	80	5.6177836E-5	15.99967	28
CTAGCGG	220	0.0	15.99967	29
>>END_MODULE