FastQCFastQC Report
Thu 2 Feb 2017
SRR4062850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921844
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33850.36719878851519344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21480.2330112253266279No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16080.17443298432272705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13370.14503538559669532No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12170.13201799870693956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.10565779025518418No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9310.10099322661968836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTA252.8923943E-432.00417713
TCGGCTA308.4118417E-426.66870112
CGATTAC401.5918072E-424.00313414
ACCGCGC400.00448646919.9971878
TACCGGC550.001365654817.452097
GTACTAC652.0864587E-417.2451841
GTACCGG652.1029651E-417.227416
CGAGCCG1404.0017767E-1117.14509615
TCTAGCG2000.016.79854828
TGCGTAC600.002439286316.00208916
CTAGCGG2150.015.62655629
CAAGACG3000.015.4636534
CCGTGCG859.418467E-515.0607913
ACGTTTT859.43591E-515.05752129
TTAGTAC859.43591E-515.0575213
CACGTTT859.43591E-515.05752128
TAGCGGC2250.014.93204230
ACTGCGC650.00416464114.7671538
GAGACCG650.00416614314.7663525
CTTATAC1103.543555E-614.5441973
Produced by FastQC (version 0.11.5)