##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062850_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 921844 Sequences flagged as poor quality 0 Sequence length 38 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25022563470609 32.0 32.0 32.0 32.0 32.0 2 30.96927028868225 32.0 32.0 32.0 32.0 32.0 3 31.01490382320653 32.0 32.0 32.0 32.0 32.0 4 31.056652752526457 32.0 32.0 32.0 32.0 32.0 5 31.03306416269998 32.0 32.0 32.0 32.0 32.0 6 34.65870581139542 36.0 36.0 36.0 32.0 36.0 7 34.590338495450425 36.0 36.0 36.0 32.0 36.0 8 34.5828079371347 36.0 36.0 36.0 32.0 36.0 9 34.67086405075045 36.0 36.0 36.0 32.0 36.0 10 34.467252593714335 36.0 36.0 36.0 32.0 36.0 11 34.66636654358004 36.0 36.0 36.0 32.0 36.0 12 34.52538824356399 36.0 36.0 36.0 32.0 36.0 13 34.58591366869015 36.0 36.0 36.0 32.0 36.0 14 34.50752947353348 36.0 36.0 36.0 32.0 36.0 15 34.472956378736534 36.0 36.0 36.0 32.0 36.0 16 34.48003132851112 36.0 36.0 36.0 32.0 36.0 17 34.41518087659083 36.0 36.0 36.0 32.0 36.0 18 34.4489197738446 36.0 36.0 36.0 32.0 36.0 19 34.42146827445858 36.0 36.0 36.0 32.0 36.0 20 34.389301226671755 36.0 36.0 36.0 32.0 36.0 21 34.36142774699407 36.0 36.0 36.0 32.0 36.0 22 34.34289966632098 36.0 36.0 36.0 32.0 36.0 23 34.29635057558546 36.0 36.0 36.0 32.0 36.0 24 34.28727094822985 36.0 36.0 36.0 32.0 36.0 25 34.26652014874534 36.0 36.0 36.0 32.0 36.0 26 34.222306594174285 36.0 36.0 36.0 32.0 36.0 27 34.23289189928014 36.0 36.0 36.0 32.0 36.0 28 34.20265793344644 36.0 36.0 36.0 32.0 36.0 29 34.181051240773925 36.0 36.0 36.0 32.0 36.0 30 34.13444791092636 36.0 36.0 36.0 32.0 36.0 31 34.15963438499356 36.0 36.0 36.0 32.0 36.0 32 34.105810744551135 36.0 36.0 36.0 32.0 36.0 33 34.06319290465632 36.0 36.0 36.0 32.0 36.0 34 34.056085411414514 36.0 36.0 36.0 32.0 36.0 35 34.02683751263771 36.0 36.0 36.0 32.0 36.0 36 33.97799085311615 36.0 36.0 36.0 32.0 36.0 37 33.969048993105126 36.0 36.0 36.0 32.0 36.0 38 33.364004104816 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 2.0 8 2.0 9 3.0 10 9.0 11 16.0 12 6.0 13 4.0 14 168.0 15 351.0 16 399.0 17 500.0 18 621.0 19 730.0 20 998.0 21 1232.0 22 1785.0 23 2554.0 24 3578.0 25 5197.0 26 7630.0 27 10970.0 28 15658.0 29 21478.0 30 29363.0 31 40309.0 32 55027.0 33 84561.0 34 194001.0 35 444691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.89031946262075 16.986853323052028 11.479693515717909 25.64313369860931 2 16.43913822108862 20.00499267379389 37.75883214847778 25.79703695663971 3 19.093432970867088 24.241082907237498 28.387475440497926 28.278008681397488 4 12.69518410278774 15.701838315857074 35.68888570678504 35.91409187457014 5 14.521500477306256 36.615247765338886 33.35351037056322 15.509741386791633 6 33.84712514875157 35.22773499493946 17.19964115208838 13.725498704220584 7 29.930986153839477 30.688164157926938 20.980230928443426 18.40061875979016 8 28.11905740794779 32.60464657137185 19.74299255511525 19.5333034655651 9 27.892042002950618 14.50859151262692 18.392671179380372 39.20669530504209 10 15.962075642604153 26.93844343076581 31.562481355123207 25.53699957150683 11 37.143043110368616 21.45125946497147 22.09346727126771 19.3122301533922 12 25.130340827976145 23.89646935281955 28.660988008426862 22.312201810777445 13 29.905856594324053 19.70740355373183 24.98754989535402 25.39918995659009 14 23.895588211362266 19.850499330067752 25.388316598588535 30.865595859981447 15 25.309932712726418 26.79294378837876 23.10662987442096 24.79049362447386 16 25.472598816116097 25.85004860520423 24.135955699828145 24.54139687885153 17 23.92702209432787 25.731070297766696 25.499080940197093 24.84282666770834 18 24.815248757838095 24.669986330794767 26.69780207858708 23.816962832780057 19 25.244423711161257 25.2800078111439 25.989628536712377 23.485939940982465 20 25.74215749664776 24.095329430705206 25.751812812712906 24.410700259934124 21 26.901496462256475 24.213744366840462 24.66385469757229 24.220904473330773 22 25.66501292651253 24.644778567701188 25.036316828116984 24.6538916776693 23 24.264445020662478 24.400495161757544 25.679843856967256 25.655215960612725 24 24.51129842667225 25.263297228855663 25.592913786500045 24.632490557972044 25 24.855991814671313 24.52159119144056 25.57199107252327 25.050425921364862 26 24.264246343474678 25.42684779306454 25.89134586172475 24.417560001736035 27 25.115109381034834 25.132359514479262 25.26157277820451 24.490958326281394 28 24.317703739624662 24.900708088832765 25.86753629131558 24.914051880226996 29 24.131941430728904 25.37137413855955 25.994887774055236 24.501796656656307 30 24.380157668579212 25.313975702072128 26.06467514349533 24.24119148585333 31 24.684630539306728 25.25732932946833 24.987415597715714 25.07062453350923 32 24.134425738642896 25.522534114160116 25.105830380642207 25.23720976655478 33 24.07243265175934 24.931244094043976 25.706951750983738 25.289371503212948 34 24.565857511429943 25.30015124194154 25.834163319597874 24.299827927030645 35 25.26731513076877 25.02058774066499 25.762831397525133 23.949265731041105 36 24.192748652727584 25.78495503305388 25.350529523900455 24.671766790318085 37 25.016029657307083 25.58007830935123 25.20165905596981 24.202232977371875 38 24.311842503523103 25.56540493158294 25.474818637560443 24.64793392733352 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 579.0 1 470.0 2 361.0 3 361.0 4 682.0 5 1003.0 6 1003.0 7 1156.5 8 1310.0 9 1225.5 10 1141.0 11 1141.0 12 1450.5 13 1760.0 14 2143.0 15 2526.0 16 2526.0 17 3733.5 18 4941.0 19 4941.0 20 5833.0 21 6725.0 22 6804.0 23 6883.0 24 6883.0 25 7979.0 26 9075.0 27 9075.0 28 11768.5 29 14462.0 30 18236.0 31 22010.0 32 22010.0 33 26872.0 34 31734.0 35 31734.0 36 35518.0 37 39302.0 38 44698.5 39 50095.0 40 50095.0 41 54229.5 42 58364.0 43 65677.0 44 72990.0 45 72990.0 46 77660.0 47 82330.0 48 82330.0 49 85337.0 50 88344.0 51 86920.5 52 85497.0 53 85497.0 54 81255.0 55 77013.0 56 77013.0 57 74359.5 58 71706.0 59 63989.5 60 56273.0 61 56273.0 62 52423.0 63 48573.0 64 40367.5 65 32162.0 66 32162.0 67 26600.5 68 21039.0 69 21039.0 70 17036.5 71 13034.0 72 10302.0 73 7570.0 74 7570.0 75 5722.0 76 3874.0 77 3874.0 78 3701.5 79 3529.0 80 2691.0 81 1853.0 82 1853.0 83 1886.5 84 1920.0 85 1920.0 86 1272.0 87 624.0 88 542.0 89 460.0 90 460.0 91 321.5 92 183.0 93 158.5 94 134.0 95 134.0 96 121.5 97 109.0 98 109.0 99 232.5 100 356.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11704800378372045 2 0.05358824269616117 3 0.011173257080373686 4 0.0023865209297885544 5 4.339128963251917E-4 6 5.423911204064896E-4 7 0.0 8 3.254346722438938E-4 9 4.339128963251917E-4 10 9.763040167316812E-4 11 0.0026034773779511503 12 0.0032543467224389376 13 0.017898906973414157 14 0.011824126424861473 15 0.02961455517419433 16 0.014319125578731327 17 0.02711955602032448 18 0.006942606341203067 19 0.009112170822829025 20 0.00781043213385345 21 0.0075934756856908545 22 0.010305431287723302 23 0.01355977801016224 24 0.018115863421576752 25 0.021153253695853094 26 0.022129557712584774 27 0.012149561097105367 28 0.00726804101344696 29 0.014319125578731327 30 0.004013694291008023 31 0.007376519237528258 32 0.009329127270991621 33 0.011390213528536282 34 0.015403907819544304 35 0.01941760211055233 36 0.018115863421576752 37 0.01247499576934926 38 0.007701953909772152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 921844.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.01188840968489 #Duplication Level Percentage of deduplicated Percentage of total 1 82.1400980720487 51.758026936765056 2 11.995976215094641 15.117782292615553 3 3.0080873812531976 5.6863579918232325 4 1.088399018846872 2.7432831008315848 5 0.500718922452629 1.5775622433101357 6 0.28685694289717983 1.084523860522827 7 0.17052011508249426 0.7521356124229328 8 0.12209863895147506 0.6154932650067801 9 0.08074675227326981 0.4579204809318972 >10 0.46992216288665084 5.652309543400523 >50 0.06552526892462511 2.9546393479249184 >100 0.06482710712859063 8.244809635930894 >500 0.005359040748597847 2.2990784702370313 >1k 8.643614110641688E-4 1.0560772182766995 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 3385 0.36719878851519344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 2148 0.2330112253266279 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 1608 0.17443298432272705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 1337 0.14503538559669532 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 1217 0.13201799870693956 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 974 0.10565779025518418 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC 931 0.10099322661968836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0847822408129792E-4 0.0 10 0.0 0.0 0.0 1.0847822408129792E-4 0.0 11 0.0 0.0 0.0 2.1695644816259585E-4 0.0 12 0.0 0.0 0.0 2.1695644816259585E-4 4.339128963251917E-4 13 0.0 0.0 0.0 2.1695644816259585E-4 4.339128963251917E-4 14 0.0 0.0 0.0 3.254346722438938E-4 4.339128963251917E-4 15 0.0 0.0 0.0 3.254346722438938E-4 4.339128963251917E-4 16 0.0 0.0 0.0 3.254346722438938E-4 4.339128963251917E-4 17 0.0 0.0 0.0 3.254346722438938E-4 4.339128963251917E-4 18 0.0 0.0 0.0 3.254346722438938E-4 4.339128963251917E-4 19 0.0 0.0 0.0 4.339128963251917E-4 4.339128963251917E-4 20 0.0 0.0 0.0 4.339128963251917E-4 4.339128963251917E-4 21 0.0 0.0 0.0 7.593475685690854E-4 4.339128963251917E-4 22 0.0 0.0 0.0 0.0014102169130568729 6.508693444877876E-4 23 0.0 0.0 0.0 0.0030373902742763417 6.508693444877876E-4 24 0.0 0.0 0.0 0.004989998307739704 6.508693444877876E-4 25 0.0 0.0 0.0 0.005857824100390088 6.508693444877876E-4 26 0.0 0.0 0.0 0.0075934756856908545 7.593475685690854E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGATTA 25 2.8923943E-4 32.004177 13 TCGGCTA 30 8.4118417E-4 26.668701 12 CGATTAC 40 1.5918072E-4 24.003134 14 ACCGCGC 40 0.004486469 19.997187 8 TACCGGC 55 0.0013656548 17.45209 7 GTACTAC 65 2.0864587E-4 17.245184 1 GTACCGG 65 2.1029651E-4 17.22741 6 CGAGCCG 140 4.0017767E-11 17.145096 15 TCTAGCG 200 0.0 16.798548 28 TGCGTAC 60 0.0024392863 16.002089 16 CTAGCGG 215 0.0 15.626556 29 CAAGACG 300 0.0 15.463653 4 CCGTGCG 85 9.418467E-5 15.06079 13 ACGTTTT 85 9.43591E-5 15.057521 29 TTAGTAC 85 9.43591E-5 15.057521 3 CACGTTT 85 9.43591E-5 15.057521 28 TAGCGGC 225 0.0 14.932042 30 ACTGCGC 65 0.004164641 14.767153 8 GAGACCG 65 0.004166143 14.766352 5 CTTATAC 110 3.543555E-6 14.544197 3 >>END_MODULE