FastQCFastQC Report
Thu 2 Feb 2017
SRR4062850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921844
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41920.45474071534880084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28860.3130681546986258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17550.19037928326267786No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15190.16477842237949153No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14980.16250037967378428No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12810.13896060504814264No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9650.1046814862384525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1750.021.02796628
CTAGCGG1750.021.02796629
TACGCTG400.004480751720.0015955
TAGCGGC1950.018.8712530
AGCGGCG2300.018.08643531
GACCGTC450.0088449217.7791947
TAACCCG1006.8119334E-817.59949323
CGGTCCA2200.017.45498810
CGTCGTA2100.016.76233110
CGCCGGT2200.016.7286057
GTATTAG2050.016.3924391
ACCGTCG2000.016.0012748
TTGATTA1255.1342795E-815.35955825
GTCGTAG2100.015.23848211
ATCACGA1807.2759576E-1215.11067625
GTAAGAC859.426916E-515.0592073
GGTCTAA756.236352E-414.9353331
TGCGTAC650.004160044214.76960711
TCACGAA1851.0913936E-1114.70227826
ATACCGT2300.014.609866
Produced by FastQC (version 0.11.5)