##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	921844
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15975913495125	32.0	32.0	32.0	32.0	32.0
2	31.291101314322162	32.0	32.0	32.0	32.0	32.0
3	31.36778240136075	32.0	32.0	32.0	32.0	32.0
4	31.472188352910038	32.0	32.0	32.0	32.0	32.0
5	31.382693818042966	32.0	32.0	32.0	32.0	32.0
6	34.913425698925195	36.0	36.0	36.0	36.0	36.0
7	34.94803025240713	36.0	36.0	36.0	36.0	36.0
8	34.88713708610133	36.0	36.0	36.0	32.0	36.0
9	34.99279379157428	36.0	36.0	36.0	36.0	36.0
10	34.83946524574657	36.0	36.0	36.0	32.0	36.0
11	35.011076711460944	36.0	36.0	36.0	36.0	36.0
12	34.91143078438434	36.0	36.0	36.0	32.0	36.0
13	34.960442330806515	36.0	36.0	36.0	36.0	36.0
14	34.91158590824478	36.0	36.0	36.0	32.0	36.0
15	34.881817313992386	36.0	36.0	36.0	32.0	36.0
16	34.88681816012254	36.0	36.0	36.0	32.0	36.0
17	34.85626743787452	36.0	36.0	36.0	32.0	36.0
18	34.84435110495919	36.0	36.0	36.0	32.0	36.0
19	34.844114622430695	36.0	36.0	36.0	32.0	36.0
20	34.82416656180438	36.0	36.0	36.0	32.0	36.0
21	34.814777771510144	36.0	36.0	36.0	32.0	36.0
22	34.79086374701142	36.0	36.0	36.0	32.0	36.0
23	34.73413072059915	36.0	36.0	36.0	32.0	36.0
24	34.713332190696036	36.0	36.0	36.0	32.0	36.0
25	34.694260634120305	36.0	36.0	36.0	32.0	36.0
26	34.62458724035737	36.0	36.0	36.0	32.0	36.0
27	34.60656683777299	36.0	36.0	36.0	32.0	36.0
28	34.57897214713118	36.0	36.0	36.0	32.0	36.0
29	34.53813768924026	36.0	36.0	36.0	32.0	36.0
30	34.51177422644179	36.0	36.0	36.0	32.0	36.0
31	34.504610324523455	36.0	36.0	36.0	32.0	36.0
32	34.46866714975636	36.0	36.0	36.0	32.0	36.0
33	34.42635847280017	36.0	36.0	36.0	32.0	36.0
34	34.41578835464569	36.0	36.0	36.0	32.0	36.0
35	34.36270128134478	36.0	36.0	36.0	32.0	36.0
36	34.33569128833078	36.0	36.0	36.0	32.0	36.0
37	34.32025592182625	36.0	36.0	36.0	32.0	36.0
38	33.90646573606814	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	21.0
22	63.0
23	222.0
24	606.0
25	1531.0
26	3229.0
27	6318.0
28	11136.0
29	17475.0
30	26227.0
31	38150.0
32	54347.0
33	86516.0
34	209068.0
35	466929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09986623169365	18.285140218043423	12.199061994503891	26.415931555759038
2	15.471923666043278	21.045100906444038	36.98141984977936	26.501555577733328
3	18.15589188626275	25.00835282325426	28.661031584519726	28.174723705963267
4	12.162253049322878	16.512229834982925	35.821896112574365	35.50362100311983
5	13.955304753629413	37.136150081955385	33.20619671679451	15.70234844762069
6	33.762112989116346	35.716896948606106	16.867333954553985	13.653656107723567
7	29.57884140163456	30.94827941568909	20.897706538459033	18.575172644217314
8	27.94209696718546	32.91759644267789	19.565705557610674	19.57460103252598
9	27.654625810636947	14.200276413909219	18.317737137749155	39.82736063770468
10	15.738808204496243	27.078677769147657	31.703470031545745	25.479043994810358
11	37.20485702414518	21.31028564795579	22.025734460549426	19.459122867349606
12	24.894613176661508	23.99695392485914	28.64916113607321	22.45927176240614
13	30.10997522357362	19.593987702908517	24.94901523468179	25.34702183883607
14	23.82810974524974	19.779160031415294	25.412651164405258	30.98007905892971
15	25.261432520035925	26.910301526071656	23.044571532710524	24.78369442118189
16	25.523624387640425	25.784731473004108	24.219173743062818	24.472470396292646
17	23.95123252958201	25.654666082330635	25.55096090010891	24.843140487978445
18	24.831641796225824	24.512824295650894	26.802257214886684	23.853276693236598
19	25.308512069287207	25.1270280003992	26.011559439558102	23.552900490755484
20	25.679073682748925	23.868246688159818	25.996697922858964	24.45598170623229
21	26.698147081444457	24.190778690080634	24.850652442986494	24.26042178548842
22	25.525360039225724	24.49850517007216	25.39041312846859	24.585721662233524
23	24.115169285876227	24.149123006846068	25.985878289470115	25.74982941780759
24	24.495684736246044	25.07105323677325	25.92087164422614	24.512390382754564
25	24.864076785226132	24.24032699675867	25.873792094974856	25.021804123040344
26	24.33735933055773	25.132940352034293	26.186917063878624	24.342783253529348
27	25.108044780005205	24.784127397379155	25.48066909658943	24.627158726026206
28	24.254863078785565	24.629329908314205	26.200636984131805	24.915170028768426
29	24.18576245004578	24.926343285848798	26.283188912657675	24.604705351447752
30	24.35824282633504	24.919834592403923	26.463262764632628	24.258659816628413
31	24.631716429243994	24.94923219112995	25.273039690012627	25.146011689613427
32	24.252476557855775	25.091230186452375	25.39529464855225	25.2609986071396
33	24.08899987416526	24.5771518825311	26.07046311523425	25.263385128069395
34	24.625207735799115	24.824916146332786	26.201396331700373	24.348479786167726
35	25.350601620230755	24.671961850378153	25.999301400236916	23.978135129154175
36	24.307800452137236	25.31697337076555	25.657378037932666	24.717848139164545
37	25.06335128286348	25.102403443532744	25.468300493358964	24.365944780244813
38	24.278294375187127	25.16944298601499	25.705759325872922	24.846503312924963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	338.0
1	250.5
2	163.0
3	163.0
4	596.5
5	1030.0
6	1030.0
7	1143.0
8	1256.0
9	1285.5
10	1315.0
11	1315.0
12	1599.5
13	1884.0
14	2353.5
15	2823.0
16	2823.0
17	4273.5
18	5724.0
19	5724.0
20	6538.5
21	7353.0
22	7245.0
23	7137.0
24	7137.0
25	8054.5
26	8972.0
27	8972.0
28	11581.5
29	14191.0
30	17745.5
31	21300.0
32	21300.0
33	26464.5
34	31629.0
35	31629.0
36	35300.0
37	38971.0
38	44750.5
39	50530.0
40	50530.0
41	54171.0
42	57812.0
43	65304.0
44	72796.0
45	72796.0
46	78217.5
47	83639.0
48	83639.0
49	86061.5
50	88484.0
51	86920.0
52	85356.0
53	85356.0
54	81543.5
55	77731.0
56	77731.0
57	74908.5
58	72086.0
59	63902.0
60	55718.0
61	55718.0
62	52119.0
63	48520.0
64	40181.0
65	31842.0
66	31842.0
67	26585.5
68	21329.0
69	21329.0
70	17188.5
71	13048.0
72	10294.0
73	7540.0
74	7540.0
75	5632.5
76	3725.0
77	3725.0
78	3539.5
79	3354.0
80	2551.5
81	1749.0
82	1749.0
83	1673.0
84	1597.0
85	1597.0
86	1048.5
87	500.0
88	406.0
89	312.0
90	312.0
91	184.0
92	56.0
93	37.5
94	19.0
95	19.0
96	13.0
97	7.0
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01095630063221109
2	0.0
3	0.0
4	0.0
5	1.0847822408129792E-4
6	3.254346722438938E-4
7	6.508693444877876E-4
8	0.002928912050195044
9	0.0041221725150893205
10	8.678257926503834E-4
11	0.004881520083658406
12	2.1695644816259585E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0847822408129792E-4
22	0.0
23	1.0847822408129792E-4
24	0.0
25	0.0
26	2.1695644816259585E-4
27	4.339128963251917E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	921844.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.587968927610994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.35617045784981	50.10561298227477
2	12.458225159866586	15.345535680780898
3	3.187243595875803	5.888875786425782
4	1.1236718714741079	2.768186732207114
5	0.5420389358235399	1.669153856852775
6	0.30350293817981483	1.1215277715634246
7	0.18056962154855347	0.7784641368841951
8	0.12850284145601615	0.6331383205362275
9	0.0846606456993235	0.4692669495049277
>10	0.4891588232086385	5.683226459016239
>50	0.07012091537742736	3.063858254889859
>100	0.06958311194531178	8.748272765679307
>500	0.005488744122872172	2.293005738445801
>1k	0.0010623375721688073	1.4318745649387394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4192	0.45474071534880084	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2886	0.3130681546986258	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1755	0.19037928326267786	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1519	0.16477842237949153	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1498	0.16250037967378428	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1281	0.13896060504814264	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	965	0.1046814862384525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0847822408129792E-4	0.0
10	0.0	0.0	0.0	1.0847822408129792E-4	0.0
11	0.0	0.0	0.0	1.0847822408129792E-4	0.0
12	0.0	0.0	0.0	1.0847822408129792E-4	0.0
13	0.0	0.0	0.0	1.0847822408129792E-4	0.0
14	0.0	0.0	0.0	2.1695644816259585E-4	0.0
15	0.0	0.0	0.0	2.1695644816259585E-4	1.0847822408129792E-4
16	0.0	0.0	0.0	2.1695644816259585E-4	1.0847822408129792E-4
17	0.0	0.0	0.0	2.1695644816259585E-4	1.0847822408129792E-4
18	0.0	0.0	0.0	2.1695644816259585E-4	2.1695644816259585E-4
19	0.0	0.0	0.0	4.339128963251917E-4	2.1695644816259585E-4
20	0.0	0.0	0.0	5.423911204064896E-4	2.1695644816259585E-4
21	0.0	0.0	0.0	8.678257926503834E-4	2.1695644816259585E-4
22	0.0	0.0	0.0	0.0015186951371381709	2.1695644816259585E-4
23	0.0	0.0	0.0	0.002928912050195044	2.1695644816259585E-4
24	0.0	0.0	0.0	0.004989998307739704	2.1695644816259585E-4
25	0.0	0.0	0.0	0.00574934587630879	2.1695644816259585E-4
26	0.0	0.0	0.0	0.007918910357934749	2.1695644816259585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	175	0.0	21.027966	28
CTAGCGG	175	0.0	21.027966	29
TACGCTG	40	0.0044807517	20.001595	5
TAGCGGC	195	0.0	18.87125	30
AGCGGCG	230	0.0	18.086435	31
GACCGTC	45	0.00884492	17.779194	7
TAACCCG	100	6.8119334E-8	17.599493	23
CGGTCCA	220	0.0	17.454988	10
CGTCGTA	210	0.0	16.762331	10
CGCCGGT	220	0.0	16.728605	7
GTATTAG	205	0.0	16.392439	1
ACCGTCG	200	0.0	16.001274	8
TTGATTA	125	5.1342795E-8	15.359558	25
GTCGTAG	210	0.0	15.238482	11
ATCACGA	180	7.2759576E-12	15.110676	25
GTAAGAC	85	9.426916E-5	15.059207	3
GGTCTAA	75	6.236352E-4	14.935333	1
TGCGTAC	65	0.0041600442	14.769607	11
TCACGAA	185	1.0913936E-11	14.702278	26
ATACCGT	230	0.0	14.60986	6
>>END_MODULE