FastQCFastQC Report
Thu 2 Feb 2017
SRR4062849_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062849_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239629
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC39860.17797590583083178No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT39650.17703825053167288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36380.16243761801619822No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA34260.1529717645199272No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC33420.14922114332329148No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33210.14828348802413258No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC32970.14721188196795096No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32200.14377381253770158No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT32150.14355056127599705No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG29070.1297982835549995No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG28890.1289945790128633No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG28240.12609231261070472No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA28200.12591371160134113No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC27850.12435095276940958No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG27410.12238634166640992No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC26720.11930547425488774No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC26210.1170283113855018No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA24000.10716060561816265No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC23520.10501739350579939No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23430.1046155412347313No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC22930.10238302861768622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG7650.019.45057329
TCTAGCG7700.019.11605628
TAGCGGC8300.017.92733630
GTATCAA35300.017.0577551
TACCGTC7350.016.9788577
AAGACGG9050.016.6147275
ACCGTCG7700.016.4148758
ATACCGT7800.015.9960936
CAAGACG9750.015.9144174
GCGCAAG9200.015.8402581
CGTCGTA7850.015.69428910
CATCGTT6900.015.53555928
TAGGACG3300.015.5117094
AGCGGCG9750.015.42531831
ATCGTTT6750.015.40708529
GTTATTC5400.015.4053653
GTCGTAG8200.015.21953311
CACGCGG859.4420306E-515.05817125
CCGTCGT8250.014.9333549
GCAAGAC11050.014.6224963
Produced by FastQC (version 0.11.5)