##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062849_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2239629
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260219438130154	32.0	32.0	32.0	32.0	32.0
2	30.846865708561552	32.0	32.0	32.0	32.0	32.0
3	30.89121948322691	32.0	32.0	32.0	32.0	32.0
4	30.967009268052877	32.0	32.0	32.0	32.0	32.0
5	30.84417017282773	32.0	32.0	32.0	32.0	32.0
6	34.52051746070443	36.0	36.0	36.0	32.0	36.0
7	34.45224142034239	36.0	36.0	36.0	32.0	36.0
8	34.43256226812566	36.0	36.0	36.0	32.0	36.0
9	34.60467604232665	36.0	36.0	36.0	32.0	36.0
10	34.28515392504741	36.0	36.0	36.0	32.0	36.0
11	34.570315440637714	36.0	36.0	36.0	32.0	36.0
12	34.36449206542691	36.0	36.0	36.0	32.0	36.0
13	34.47426515730954	36.0	36.0	36.0	32.0	36.0
14	34.355048090554284	36.0	36.0	36.0	32.0	36.0
15	34.27969364568864	36.0	36.0	36.0	32.0	36.0
16	34.310189768037475	36.0	36.0	36.0	32.0	36.0
17	34.21999893732399	36.0	36.0	36.0	32.0	36.0
18	34.256546508372594	36.0	36.0	36.0	32.0	36.0
19	34.24119753762788	36.0	36.0	36.0	32.0	36.0
20	34.231797766505075	36.0	36.0	36.0	32.0	36.0
21	34.21417788392631	36.0	36.0	36.0	32.0	36.0
22	34.18251772949895	36.0	36.0	36.0	32.0	36.0
23	34.12894233821763	36.0	36.0	36.0	32.0	36.0
24	34.10552060184968	36.0	36.0	36.0	32.0	36.0
25	34.07993020272554	36.0	36.0	36.0	32.0	36.0
26	34.02988218137915	36.0	36.0	36.0	32.0	36.0
27	34.035656798514395	36.0	36.0	36.0	32.0	36.0
28	34.01750647093782	36.0	36.0	36.0	32.0	36.0
29	33.983369567013106	36.0	36.0	36.0	32.0	36.0
30	33.96052605141298	36.0	36.0	36.0	32.0	36.0
31	33.98597624874477	36.0	36.0	36.0	32.0	36.0
32	33.931113590688454	36.0	36.0	36.0	32.0	36.0
33	33.8974660535294	36.0	36.0	36.0	32.0	36.0
34	33.91382367347449	36.0	36.0	36.0	32.0	36.0
35	33.87605625753194	36.0	36.0	36.0	32.0	36.0
36	33.826063155995925	36.0	36.0	36.0	32.0	36.0
37	33.835324957839	36.0	36.0	36.0	32.0	36.0
38	33.22492341365467	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	4.0
8	4.0
9	12.0
10	19.0
11	16.0
12	18.0
13	11.0
14	137.0
15	388.0
16	529.0
17	690.0
18	938.0
19	1151.0
20	1680.0
21	2590.0
22	3981.0
23	6292.0
24	9931.0
25	14926.0
26	22147.0
27	31868.0
28	44467.0
29	60292.0
30	80822.0
31	110435.0
32	150395.0
33	227022.0
34	501456.0
35	967405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.99491359413271	16.684957998482748	11.16166124930318	26.15846715808136
2	17.507432714666404	18.942868056191546	36.52521985422721	27.02447937491484
3	19.436885261274842	22.65770758330706	27.5994187640609	30.305988391357197
4	12.962952213530562	14.648590553441803	34.80766594614048	37.580791286887155
5	15.554204139441138	35.57084977581078	32.72533003278226	16.149616051965822
6	35.79822514929955	34.35097393536864	16.183155662220237	13.667645253111571
7	31.90017802071506	29.398198896512678	19.91371777532598	18.787905307446284
8	29.045494751120838	31.85802419248614	18.83657993919507	20.259901117197956
9	27.628647882833747	14.081400379796403	18.162581972574824	40.12736976479502
10	16.799807109704123	25.328326520406407	30.02819681061254	27.84366955927693
11	38.80983881345556	20.58092168215544	21.337671053354224	19.27156845103477
12	25.966692698461173	22.9029782069675	27.28319600780329	23.847133086768036
13	29.75601216813171	18.406621378542862	24.91050742824071	26.926859025084717
14	24.285272206764343	19.408471572314067	23.72186714500502	32.58438907591656
15	25.836041647220547	26.541505868670928	21.40228208475485	26.220170399353666
16	26.955206538276215	24.897035635516644	22.83176451202505	25.31599331418209
17	25.022921429404004	25.269351916401206	24.09958864240382	25.60813801179097
18	26.294903618724074	23.583394619580965	25.260382828062273	24.86131893363269
19	26.406733871417888	24.118289293010484	24.399031001261484	25.075945834310147
20	26.658170669519706	23.339819940825706	23.810999488275435	26.191009901379154
21	28.077862857204096	23.30281726618191	23.437804200625504	25.181515675988493
22	26.941396954708217	23.329941141982186	24.09043799456743	25.638223908742162
23	24.982473093407474	23.168828988297832	24.9483123710042	26.900385547290494
24	25.61069334644458	24.167161241084823	24.343645042488742	25.878500369981854
25	25.69506872647359	23.62892036089046	24.40271002782642	26.273300884809526
26	25.417033798125853	24.9443664654496	24.868089462028298	24.770510274396248
27	26.324417181420717	23.702576427189374	23.96434075088226	26.00866564050765
28	25.226055932377463	23.857485409624513	24.927550469080014	25.988908188918014
29	25.120770358144735	24.156483017128892	24.906737329271483	25.81600929545489
30	24.878055968336525	24.462838996370703	25.28670912753979	25.37239590775298
31	26.13558170137768	24.18103042164492	23.57361561176178	26.109772265215625
32	25.678038682297714	23.968172883453885	23.50092030539314	26.852868128855256
33	24.98739631825134	23.617401788405505	24.58939236634404	26.80580952699912
34	26.332307579636577	23.77350536700949	24.50912751096422	25.38505954238971
35	26.797585267236318	23.46710109706179	24.807145594023723	24.928168041678166
36	25.003293456092536	24.600665657715464	24.14136808603088	26.25467280016112
37	26.530688796561915	24.14994480625029	24.016068704608053	25.303297692579747
38	25.272385633756528	23.857364593082657	24.65232876320847	26.217921009952345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	104.0
2	138.0
3	138.0
4	356.0
5	574.0
6	574.0
7	727.0
8	880.0
9	867.5
10	855.0
11	855.0
12	1188.5
13	1522.0
14	2175.0
15	2828.0
16	2828.0
17	4175.0
18	5522.0
19	5522.0
20	6895.0
21	8268.0
22	9079.5
23	9891.0
24	9891.0
25	12169.0
26	14447.0
27	14447.0
28	18689.0
29	22931.0
30	29932.5
31	36934.0
32	36934.0
33	50617.5
34	64301.0
35	64301.0
36	72852.0
37	81403.0
38	95491.5
39	109580.0
40	109580.0
41	117344.5
42	125109.0
43	146130.0
44	167151.0
45	167151.0
46	171446.0
47	175741.0
48	175741.0
49	192310.5
50	208880.0
51	216595.5
52	224311.0
53	224311.0
54	215344.5
55	206378.0
56	206378.0
57	202254.0
58	198130.0
59	178510.5
60	158891.0
61	158891.0
62	152435.0
63	145979.0
64	121939.0
65	97899.0
66	97899.0
67	82403.5
68	66908.0
69	66908.0
70	53381.0
71	39854.0
72	31140.0
73	22426.0
74	22426.0
75	16819.5
76	11213.0
77	11213.0
78	11017.0
79	10821.0
80	8559.5
81	6298.0
82	6298.0
83	6459.5
84	6621.0
85	6621.0
86	4457.0
87	2293.0
88	1972.5
89	1652.0
90	1652.0
91	1201.5
92	751.0
93	665.0
94	579.0
95	579.0
96	528.5
97	478.0
98	478.0
99	800.0
100	1122.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11993057778766038
2	0.054205406341853946
3	0.011743016365656991
4	0.0027683156451358687
5	1.3395075702270332E-4
6	1.7860100936360442E-4
7	8.930050468180221E-5
8	3.5720201872720884E-4
9	3.5720201872720884E-4
10	6.251035327726154E-4
11	0.002232512617045055
12	0.002679015140454066
13	0.01504713503888367
14	0.009376552991589232
15	0.02393253525472299
16	0.01169836611331609
17	0.020449815572132705
18	0.006161734823044352
19	0.009108651477543825
20	0.006384986084748858
21	0.006831488608157869
22	0.00767984340263499
23	0.009599804253293737
24	0.014734583272497364
25	0.01741359841295143
26	0.018842406487860265
27	0.009376552991589232
28	0.006206385075385253
29	0.011385814346929783
30	0.003438069430249385
31	0.006161734823044352
32	0.006920789112839672
33	0.009108651477543825
34	0.013395075702270332
35	0.016743844627837914
36	0.015181085795906376
37	0.01093931182352077
38	0.007010089617521474
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2239629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.31556851082714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62643004938268	38.77507097862089
2	12.564668506903246	12.392675411360383
3	3.869371320730252	5.724607393839073
4	1.663617019398851	3.2816887638376833
5	0.86043100691945	2.1216322135288053
6	0.5008450337273254	1.4819674544452464
7	0.35496725962788545	1.2253788547895683
8	0.24956553781033913	0.9845973102261759
9	0.1835727707029491	0.8147695995291283
>10	0.955382146016474	8.247085245906186
>50	0.06740642445387592	2.3059229999194035
>100	0.0777420439528084	8.51755798461918
>500	0.015965452861163135	5.520595888291144
>1k	0.01003542751273111	8.606449901087082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3986	0.17797590583083178	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3965	0.17703825053167288	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3638	0.16243761801619822	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3426	0.1529717645199272	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3342	0.14922114332329148	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3321	0.14828348802413258	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3297	0.14721188196795096	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3220	0.14377381253770158	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3215	0.14355056127599705	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2907	0.1297982835549995	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2889	0.1289945790128633	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2824	0.12609231261070472	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2820	0.12591371160134113	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2785	0.12435095276940958	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2741	0.12238634166640992	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2672	0.11930547425488774	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2621	0.1170283113855018	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2400	0.10716060561816265	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2352	0.10501739350579939	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2343	0.1046155412347313	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2293	0.10238302861768622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.4650252340901105E-5	0.0	0.0	0.0
3	0.0	4.4650252340901105E-5	0.0	0.0	0.0
4	0.0	4.4650252340901105E-5	0.0	0.0	0.0
5	0.0	4.4650252340901105E-5	0.0	0.0	0.0
6	0.0	4.4650252340901105E-5	0.0	0.0	0.0
7	0.0	4.4650252340901105E-5	0.0	0.0	0.0
8	0.0	4.4650252340901105E-5	0.0	0.0	0.0
9	0.0	4.4650252340901105E-5	0.0	0.0	0.0
10	0.0	4.4650252340901105E-5	0.0	0.0	4.4650252340901105E-5
11	0.0	4.4650252340901105E-5	0.0	0.0	4.4650252340901105E-5
12	0.0	4.4650252340901105E-5	0.0	0.0	2.6790151404540663E-4
13	0.0	4.4650252340901105E-5	0.0	0.0	2.6790151404540663E-4
14	0.0	4.4650252340901105E-5	0.0	0.0	2.6790151404540663E-4
15	0.0	4.4650252340901105E-5	0.0	4.4650252340901105E-5	3.125517663863077E-4
16	0.0	4.4650252340901105E-5	0.0	8.930050468180221E-5	3.125517663863077E-4
17	0.0	4.4650252340901105E-5	0.0	8.930050468180221E-5	3.125517663863077E-4
18	0.0	4.4650252340901105E-5	0.0	8.930050468180221E-5	3.125517663863077E-4
19	0.0	4.4650252340901105E-5	0.0	8.930050468180221E-5	3.125517663863077E-4
20	0.0	4.4650252340901105E-5	0.0	1.3395075702270332E-4	3.5720201872720884E-4
21	0.0	4.4650252340901105E-5	0.0	1.3395075702270332E-4	4.018522710681099E-4
22	0.0	4.4650252340901105E-5	0.0	2.6790151404540663E-4	4.018522710681099E-4
23	0.0	4.4650252340901105E-5	0.0	6.697537851135166E-4	4.4650252340901105E-4
24	0.0	4.4650252340901105E-5	0.0	0.0010716060561816265	4.4650252340901105E-4
25	0.0	4.4650252340901105E-5	0.0	0.001294857317886132	4.4650252340901105E-4
26	0.0	4.4650252340901105E-5	0.0	0.0019199608506587475	4.911527757499121E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	765	0.0	19.450573	29
TCTAGCG	770	0.0	19.116056	28
TAGCGGC	830	0.0	17.927336	30
GTATCAA	3530	0.0	17.057755	1
TACCGTC	735	0.0	16.978857	7
AAGACGG	905	0.0	16.614727	5
ACCGTCG	770	0.0	16.414875	8
ATACCGT	780	0.0	15.996093	6
CAAGACG	975	0.0	15.914417	4
GCGCAAG	920	0.0	15.840258	1
CGTCGTA	785	0.0	15.694289	10
CATCGTT	690	0.0	15.535559	28
TAGGACG	330	0.0	15.511709	4
AGCGGCG	975	0.0	15.425318	31
ATCGTTT	675	0.0	15.407085	29
GTTATTC	540	0.0	15.405365	3
GTCGTAG	820	0.0	15.219533	11
CACGCGG	85	9.4420306E-5	15.058171	25
CCGTCGT	825	0.0	14.933354	9
GCAAGAC	1105	0.0	14.622496	3
>>END_MODULE