FastQCFastQC Report
Thu 2 Feb 2017
SRR4062849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239629
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC42550.1899868237105342No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT40450.18061027071894498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38420.17154626949374205No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG35860.16011580489447136No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC34470.1539094198190861No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA33820.15100715341692755No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC33680.1503820498841549No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC33610.1500694981177686No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG33280.14859603979051889No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT31890.14238965471513362No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG30670.13694232392954367No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30540.13636187064911198No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA29600.13216474692906727No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG26450.11809991744168341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26280.11734086315188809No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG25490.11381349321695693No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC25430.11354559170291151No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24770.11059867504841203No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC24270.10836616243136699No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC24030.10729455637518537No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA23490.1048834427487767No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23050.10291883164577705No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA22610.10095422054277739No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT22490.10041841751468658No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22470.10032911701000478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG350.002066629222.8581315
TCTAGCG7250.020.08228328
CTAGCGG7550.019.2843129
TAGCGGC7850.018.5473330
ATCGTTT7400.017.51309829
CGTTTAT7450.017.18080131
GTATCAA34600.017.0192171
CATCGTT7600.016.84170528
TCGTTTA7800.016.6149930
ATAGGCG805.6225977E-516.0006925
TGTAGGA5900.015.4579512
AGCGGCG9600.015.33297131
CGCAAGA10000.015.2003192
ATACCGT7700.015.1694886
TACCGTC7950.015.095337
ACTAGAC859.436866E-515.0591373
TTTTCGG9350.015.05846729
CAAGACG10450.015.0050984
GTATAGG2250.014.934981
CGACGGT9500.014.8220257
Produced by FastQC (version 0.11.5)