##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2239629
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223529879279113	32.0	32.0	32.0	32.0	32.0
2	31.2618549768734	32.0	32.0	32.0	32.0	32.0
3	31.34697264591591	32.0	32.0	32.0	32.0	32.0
4	31.450678215007933	32.0	32.0	32.0	32.0	32.0
5	31.359819863021954	32.0	32.0	32.0	32.0	32.0
6	34.89451243933705	36.0	36.0	36.0	36.0	36.0
7	34.915213635829865	36.0	36.0	36.0	36.0	36.0
8	34.84494530120837	36.0	36.0	36.0	32.0	36.0
9	34.967903612607266	36.0	36.0	36.0	32.0	36.0
10	34.8023002917001	36.0	36.0	36.0	32.0	36.0
11	34.979961859754454	36.0	36.0	36.0	36.0	36.0
12	34.87088977683357	36.0	36.0	36.0	32.0	36.0
13	34.936711392824435	36.0	36.0	36.0	36.0	36.0
14	34.87181760907722	36.0	36.0	36.0	32.0	36.0
15	34.83860139335577	36.0	36.0	36.0	32.0	36.0
16	34.854897395952634	36.0	36.0	36.0	32.0	36.0
17	34.8166343622091	36.0	36.0	36.0	32.0	36.0
18	34.81577350534396	36.0	36.0	36.0	32.0	36.0
19	34.814609026762916	36.0	36.0	36.0	32.0	36.0
20	34.79903010721865	36.0	36.0	36.0	32.0	36.0
21	34.791644955481466	36.0	36.0	36.0	32.0	36.0
22	34.77946436664287	36.0	36.0	36.0	32.0	36.0
23	34.71021271826718	36.0	36.0	36.0	32.0	36.0
24	34.689851310194676	36.0	36.0	36.0	32.0	36.0
25	34.65889796926187	36.0	36.0	36.0	32.0	36.0
26	34.59705960228234	36.0	36.0	36.0	32.0	36.0
27	34.586575276530176	36.0	36.0	36.0	32.0	36.0
28	34.552733510773436	36.0	36.0	36.0	32.0	36.0
29	34.52175114717661	36.0	36.0	36.0	32.0	36.0
30	34.48867736576013	36.0	36.0	36.0	32.0	36.0
31	34.49943986258438	36.0	36.0	36.0	32.0	36.0
32	34.46516007785218	36.0	36.0	36.0	32.0	36.0
33	34.43464386288979	36.0	36.0	36.0	32.0	36.0
34	34.440966338621266	36.0	36.0	36.0	32.0	36.0
35	34.40878868776927	36.0	36.0	36.0	32.0	36.0
36	34.377375895739874	36.0	36.0	36.0	32.0	36.0
37	34.37267243815828	36.0	36.0	36.0	32.0	36.0
38	33.91660761670794	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	6.0
21	43.0
22	161.0
23	521.0
24	1557.0
25	3795.0
26	8157.0
27	15596.0
28	26616.0
29	42555.0
30	63853.0
31	92838.0
32	133528.0
33	215398.0
34	523206.0
35	1111798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14428225039407	18.022443210995657	11.957685661083401	26.87558887752687
2	16.40383295626195	19.95750188982193	35.73774942189086	27.90091573202526
3	18.337456784137014	23.450937632974032	27.98767117232363	30.223934410565324
4	12.345532383056472	15.489804556828188	34.946964406588954	37.21769865352639
5	14.957394710192942	36.104210163473574	32.38144906207638	16.5569460642571
6	35.773501569681024	34.765923075583565	15.853130900815179	13.607444453920229
7	31.563401940332632	29.575203963536605	19.945338843801792	18.91605525232897
8	28.921403812399337	32.13440692413378	18.60012582677074	20.344063436696143
9	27.54756308404538	13.870497313748464	17.949259494756095	40.63268010745006
10	16.54988299307335	25.581138867419384	30.07026666684527	27.798711472661992
11	38.931440188719094	20.486854543319346	21.289125030866003	19.292580237095557
12	25.67891885568445	23.083218768125228	27.19899340381233	24.038868972377987
13	29.72433306855663	18.38148652697351	24.83663545159163	27.05754495287823
14	24.11042186004914	19.36289447939815	23.705578022074192	32.821105638478514
15	25.81793681006988	26.67013152624832	21.244411462791383	26.267520200890417
16	26.948838401360227	24.997622374062846	22.611021736189343	25.442517488387583
17	24.995657762959848	25.416307790263478	23.897975959411134	25.69005848736554
18	26.29547125885582	23.675037249473014	25.164301766051434	24.865189725619736
19	26.309000285315115	24.21642155910644	24.37832337409455	25.096254781483896
20	26.704556870803152	23.255771379991955	23.77969744096009	26.259974308244804
21	28.047056007937027	23.392579753164476	23.37646101206941	25.183903226829084
22	26.828152034380754	23.426912987665347	24.111000725567898	25.633934252386005
23	24.847295796080058	23.086062660518014	24.987944404953687	27.078697138448238
24	25.640853909285866	24.129442867546366	24.320813849079467	25.908889374088297
25	25.646995052292283	23.623975999532064	24.42201209757178	26.307016850603876
26	25.455834629679575	24.92418383767304	24.743216284132902	24.876765248514484
27	26.469508748335656	23.619970477213606	23.826746072781795	26.083774701668936
28	25.149745783788298	23.850378790415732	24.987040264258052	26.012835161537918
29	25.11442743418664	24.056841557240062	24.93788033643072	25.890850672142573
30	24.902383385819704	24.310678241798083	25.291599635475343	25.49533873690687
31	26.02444422714655	24.03023000684488	23.71531177708451	26.230013988924057
32	25.72479638368676	23.89297513114895	23.563500919125442	26.81872756603884
33	24.95815155099349	23.527289564476973	24.63309771395173	26.881461170577804
34	26.238988689644575	23.677359062594743	24.612915799893642	25.470736447867036
35	26.790285355297684	23.47902264169646	24.73650769837326	24.994184304632597
36	25.083306208305032	24.454005551812376	24.21776106667667	26.244927173205916
37	26.644457631152303	24.104661977497166	23.93132969790979	25.319550693440746
38	25.30689262095876	23.740515719805128	24.620126476417724	26.33246518281839
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	49.5
2	68.0
3	68.0
4	279.0
5	490.0
6	490.0
7	647.0
8	804.0
9	829.0
10	854.0
11	854.0
12	1164.0
13	1474.0
14	2120.5
15	2767.0
16	2767.0
17	4299.0
18	5831.0
19	5831.0
20	7012.5
21	8194.0
22	9047.5
23	9901.0
24	9901.0
25	12098.5
26	14296.0
27	14296.0
28	18592.5
29	22889.0
30	29934.0
31	36979.0
32	36979.0
33	50823.5
34	64668.0
35	64668.0
36	73493.0
37	82318.0
38	96308.0
39	110298.0
40	110298.0
41	117939.5
42	125581.0
43	146230.0
44	166879.0
45	166879.0
46	171295.5
47	175712.0
48	175712.0
49	192382.5
50	209053.0
51	216204.5
52	223356.0
53	223356.0
54	215499.5
55	207643.0
56	207643.0
57	203602.0
58	199561.0
59	179619.5
60	159678.0
61	159678.0
62	152631.0
63	145584.0
64	121547.5
65	97511.0
66	97511.0
67	82611.5
68	67712.0
69	67712.0
70	54252.5
71	40793.0
72	31645.5
73	22498.0
74	22498.0
75	16594.5
76	10691.0
77	10691.0
78	10762.5
79	10834.0
80	8380.0
81	5926.0
82	5926.0
83	5842.5
84	5759.0
85	5759.0
86	3706.5
87	1654.0
88	1330.5
89	1007.0
90	1007.0
91	607.0
92	207.0
93	138.0
94	69.0
95	69.0
96	47.0
97	25.0
98	25.0
99	29.5
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00888540021583932
2	0.0
3	0.0
4	4.4650252340901105E-5
5	4.4650252340901105E-5
6	8.930050468180221E-5
7	5.804532804317144E-4
8	0.0018753105983178463
9	0.003438069430249385
10	8.037045421362198E-4
11	0.004911527757499121
12	8.930050468180221E-5
13	2.6790151404540663E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.7860100936360442E-4
23	2.232512617045055E-4
24	0.0
25	2.6790151404540663E-4
26	4.4650252340901105E-5
27	1.3395075702270332E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.930050468180221E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2239629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.16458296294111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9754227642107	36.305137130700814
2	13.513818149090964	12.747471944777999
3	4.154296939816258	5.878070480119687
4	1.7500536950332441	3.3016221075598833
5	0.9571382100739354	2.257151225801654
6	0.5588338893817347	1.5814300402948727
7	0.3959825454843316	1.307344613286065
8	0.2710243223348134	1.0226199308588158
9	0.18799460795977826	0.7980018555334095
>10	1.0511404810723526	8.657665026321341
>50	0.07221040784717182	2.3584574782487127
>100	0.08318364683338704	8.593922278290659
>500	0.01764542263817362	5.807498699217202
>1k	0.011254918223267497	9.38360718898894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4255	0.1899868237105342	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4045	0.18061027071894498	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3842	0.17154626949374205	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3586	0.16011580489447136	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3447	0.1539094198190861	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3382	0.15100715341692755	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3368	0.1503820498841549	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3361	0.1500694981177686	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3328	0.14859603979051889	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3189	0.14238965471513362	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3067	0.13694232392954367	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3054	0.13636187064911198	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2960	0.13216474692906727	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2645	0.11809991744168341	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2628	0.11734086315188809	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2549	0.11381349321695693	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2543	0.11354559170291151	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2477	0.11059867504841203	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2427	0.10836616243136699	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2403	0.10729455637518537	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2349	0.1048834427487767	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2305	0.10291883164577705	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2261	0.10095422054277739	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2249	0.10041841751468658	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2247	0.10032911701000478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.4650252340901105E-5
2	0.0	0.0	0.0	0.0	4.4650252340901105E-5
3	0.0	0.0	0.0	0.0	4.4650252340901105E-5
4	0.0	0.0	0.0	0.0	4.4650252340901105E-5
5	0.0	0.0	0.0	0.0	4.4650252340901105E-5
6	0.0	0.0	0.0	0.0	8.930050468180221E-5
7	0.0	0.0	0.0	0.0	8.930050468180221E-5
8	0.0	0.0	0.0	0.0	8.930050468180221E-5
9	0.0	0.0	0.0	0.0	8.930050468180221E-5
10	0.0	0.0	0.0	0.0	8.930050468180221E-5
11	0.0	0.0	0.0	0.0	8.930050468180221E-5
12	0.0	0.0	0.0	0.0	4.911527757499121E-4
13	0.0	0.0	0.0	0.0	4.911527757499121E-4
14	0.0	0.0	0.0	0.0	4.911527757499121E-4
15	0.0	0.0	0.0	4.4650252340901105E-5	5.804532804317144E-4
16	0.0	0.0	0.0	8.930050468180221E-5	6.251035327726154E-4
17	0.0	0.0	0.0	8.930050468180221E-5	6.251035327726154E-4
18	0.0	0.0	0.0	1.3395075702270332E-4	7.144040374544177E-4
19	0.0	0.0	0.0	1.7860100936360442E-4	7.144040374544177E-4
20	0.0	0.0	0.0	2.6790151404540663E-4	8.48354794477121E-4
21	0.0	0.0	0.0	3.5720201872720884E-4	8.930050468180221E-4
22	0.0	0.0	0.0	5.358030280908133E-4	8.930050468180221E-4
23	0.0	0.0	0.0	8.930050468180221E-4	8.930050468180221E-4
24	0.0	0.0	0.0	0.0012502070655452308	9.376552991589232E-4
25	0.0	0.0	0.0	0.0015181085795906376	9.376552991589232E-4
26	0.0	0.0	0.0	0.002053911607681451	9.376552991589232E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	35	0.0020666292	22.858131	5
TCTAGCG	725	0.0	20.082283	28
CTAGCGG	755	0.0	19.28431	29
TAGCGGC	785	0.0	18.54733	30
ATCGTTT	740	0.0	17.513098	29
CGTTTAT	745	0.0	17.180801	31
GTATCAA	3460	0.0	17.019217	1
CATCGTT	760	0.0	16.841705	28
TCGTTTA	780	0.0	16.61499	30
ATAGGCG	80	5.6225977E-5	16.000692	5
TGTAGGA	590	0.0	15.457951	2
AGCGGCG	960	0.0	15.332971	31
CGCAAGA	1000	0.0	15.200319	2
ATACCGT	770	0.0	15.169488	6
TACCGTC	795	0.0	15.09533	7
ACTAGAC	85	9.436866E-5	15.059137	3
TTTTCGG	935	0.0	15.058467	29
CAAGACG	1045	0.0	15.005098	4
GTATAGG	225	0.0	14.93498	1
CGACGGT	950	0.0	14.822025	7
>>END_MODULE