FastQCFastQC Report
Thu 2 Feb 2017
SRR4062848_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062848_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366246
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40130.29372455619266225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25160.18415424455039575No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18910.13840845645659713No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18690.13679820471569543No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18350.13430963384339278No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18000.13174786971014005No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17530.12830778644548638No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17490.12801501340168608No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16980.1242821570932321No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16930.12391619078848172No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16160.11828030969532573No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15410.1127908151240699No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14750.10796005990136477No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14540.10642300142141314No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14100.10320249793960971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14100.10320249793960971No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT13790.1009335068501573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC450.00885372917.7768633
CGCGGTC2650.017.51109710
GTATTAA1201.6370905E-917.3514941
GGTCCTA2950.016.27273213
ACCGTCG3550.016.2245188
TACCGTC3550.016.2245187
ATACCGT3650.016.215976
GTACGGA600.002425210816.0167641
AACCGCG2900.015.9991777
GAACCGC2950.015.7257016
GTATAAG1551.8553692E-1015.5000951
GTGTAGG1255.0731614E-815.3760941
TAAACGC4100.015.21872928
CGTCGTA3850.014.962460510
CGTCTTA1501.9699655E-914.93420515
GTCGTAA1308.3180566E-814.76847230
TACGCAG650.004167590814.7663095
CGCCCCT3600.014.6637645
GCGCCCC3500.014.62728321
TGACTCG2300.014.6058046
Produced by FastQC (version 0.11.5)