##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062848_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1366246
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2368724226823	32.0	32.0	32.0	32.0	32.0
2	30.90654757635155	32.0	32.0	32.0	32.0	32.0
3	30.95503518400054	32.0	32.0	32.0	32.0	32.0
4	31.00878319131401	32.0	32.0	32.0	32.0	32.0
5	30.96331992920748	32.0	32.0	32.0	32.0	32.0
6	34.593254069911275	36.0	36.0	36.0	32.0	36.0
7	34.51488018995115	36.0	36.0	36.0	32.0	36.0
8	34.5017332164193	36.0	36.0	36.0	32.0	36.0
9	34.60865685974561	36.0	36.0	36.0	32.0	36.0
10	34.374758279255715	36.0	36.0	36.0	32.0	36.0
11	34.60260231319982	36.0	36.0	36.0	32.0	36.0
12	34.431273723765706	36.0	36.0	36.0	32.0	36.0
13	34.51263388877259	36.0	36.0	36.0	32.0	36.0
14	34.424883220152154	36.0	36.0	36.0	32.0	36.0
15	34.37990888902877	36.0	36.0	36.0	32.0	36.0
16	34.39777024049842	36.0	36.0	36.0	32.0	36.0
17	34.3173630517491	36.0	36.0	36.0	32.0	36.0
18	34.34259130493337	36.0	36.0	36.0	32.0	36.0
19	34.329379921331885	36.0	36.0	36.0	32.0	36.0
20	34.302807839876564	36.0	36.0	36.0	32.0	36.0
21	34.29358768479469	36.0	36.0	36.0	32.0	36.0
22	34.2687671180739	36.0	36.0	36.0	32.0	36.0
23	34.21935215180868	36.0	36.0	36.0	32.0	36.0
24	34.20612759342022	36.0	36.0	36.0	32.0	36.0
25	34.18966350130211	36.0	36.0	36.0	32.0	36.0
26	34.142633171478636	36.0	36.0	36.0	32.0	36.0
27	34.15233420628496	36.0	36.0	36.0	32.0	36.0
28	34.1340556532279	36.0	36.0	36.0	32.0	36.0
29	34.101653728537904	36.0	36.0	36.0	32.0	36.0
30	34.06811877216841	36.0	36.0	36.0	32.0	36.0
31	34.08836622394503	36.0	36.0	36.0	32.0	36.0
32	34.04584825865913	36.0	36.0	36.0	32.0	36.0
33	33.9968644007009	36.0	36.0	36.0	32.0	36.0
34	34.00937386092988	36.0	36.0	36.0	32.0	36.0
35	33.96386741479938	36.0	36.0	36.0	32.0	36.0
36	33.92638441393424	36.0	36.0	36.0	32.0	36.0
37	33.930085797140485	36.0	36.0	36.0	32.0	36.0
38	33.336029529089195	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	4.0
10	8.0
11	16.0
12	4.0
13	6.0
14	168.0
15	343.0
16	503.0
17	544.0
18	747.0
19	908.0
20	1244.0
21	1739.0
22	2649.0
23	3978.0
24	6052.0
25	8683.0
26	12742.0
27	18430.0
28	25599.0
29	34575.0
30	46269.0
31	62278.0
32	84507.0
33	127034.0
34	286455.0
35	640754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.95826286277073	16.858927948551848	11.258386851878448	25.92442233679897
2	17.041673959322722	19.795258833756	36.78713524209299	26.375931964828283
3	19.04706829661079	24.05446160603177	28.06207451870288	28.836395578654567
4	12.82229766228519	15.374694592177168	35.4639783458717	36.33902939966593
5	15.130031773264346	35.95407259177174	33.052099809477525	15.863795825486388
6	34.318808120066315	35.01107048882155	16.92646633656482	13.743655054547318
7	30.546402331644522	30.22859719259928	20.813894423112675	18.41110605264352
8	28.600319124019208	31.795365382363272	19.588212905492448	20.016102588125072
9	27.972821838558854	14.247747840063706	18.297097736568286	39.48233258480916
10	16.309823032297448	25.982881459892056	30.781679326735166	26.925616181075334
11	37.70701187065683	21.070525667056547	21.94966279371056	19.272799668576063
12	25.619269714816067	23.48437936885067	27.962254594109524	22.934096322223734
13	29.62333680807702	19.01485160111504	25.00102485417789	26.360786736630043
14	24.381074241810914	19.53229481808659	24.567002679858312	31.519628260244183
15	25.565801177982202	26.314614227301515	22.365774821637093	25.75380977307919
16	26.343486634492137	25.21508450022729	23.165587065576158	25.275841799704413
17	24.5175346443106	25.25753082280283	24.86491116458619	25.36002336830037
18	25.621648922348328	24.069347406792005	25.70114151658109	24.607862154278582
19	26.019346409056137	24.60148812900534	25.12193068817732	24.257234773761205
20	26.295918382285127	23.59165084388958	24.60828562101167	25.504145152813624
21	27.648695891501923	23.67327309566195	23.96862995408976	24.70940105874637
22	26.399359364283242	23.612023045903687	24.50907408668053	25.47954350313254
23	24.6720420465484	23.510857510952672	25.248644869903853	26.568455572595077
24	25.19188522244045	24.709619186984966	24.746513856364075	25.35198173421051
25	25.28209813369409	23.890750753652597	24.830565426123012	25.9965856865303
26	25.046395228044897	24.937462526931196	25.27260628076086	24.743535964263046
27	25.9313237490118	24.263827482212395	24.306210288993647	25.498638479782155
28	24.889161182368557	24.190933359098736	25.328417411264898	25.59148804726781
29	24.75374795033966	24.562251112672755	25.329775708596863	25.354225228390725
30	24.831852227587433	24.65252388177797	25.532330704892146	24.983293185742443
31	25.37442786339138	24.595670610349806	24.169070622500737	25.860830903758075
32	25.02163000180067	24.54657513991793	24.111998758562333	26.319796099719067
33	24.745376368643193	24.051227771214243	24.886724142726848	26.316671717415712
34	25.64976681231594	24.143837747343287	25.05499364233233	25.151401798008443
35	26.41174623230536	24.03886909498463	24.88021780045841	24.669166872251598
36	25.023644849961347	24.968156316615524	24.41114807093162	25.59705076249151
37	25.956645958095336	24.83374216108472	24.4787171665931	24.73089471422684
38	24.974398951802687	24.515666231137757	24.98999015485066	25.5199446622089
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	390.0
1	351.5
2	313.0
3	313.0
4	680.5
5	1048.0
6	1048.0
7	1195.5
8	1343.0
9	1305.0
10	1267.0
11	1267.0
12	1664.0
13	2061.0
14	2594.5
15	3128.0
16	3128.0
17	4464.0
18	5800.0
19	5800.0
20	6940.5
21	8081.0
22	8370.5
23	8660.0
24	8660.0
25	9743.5
26	10827.0
27	10827.0
28	14156.5
29	17486.0
30	21582.5
31	25679.0
32	25679.0
33	33874.5
34	42070.0
35	42070.0
36	47042.0
37	52014.0
38	60088.5
39	68163.0
40	68163.0
41	73619.0
42	79075.0
43	91017.0
44	102959.0
45	102959.0
46	108207.5
47	113456.0
48	113456.0
49	121105.5
50	128755.0
51	129712.5
52	130670.0
53	130670.0
54	125828.5
55	120987.0
56	120987.0
57	118626.0
58	116265.0
59	104392.5
60	92520.0
61	92520.0
62	88131.5
63	83743.0
64	68975.0
65	54207.0
66	54207.0
67	45414.0
68	36621.0
69	36621.0
70	29315.0
71	22009.0
72	17495.0
73	12981.0
74	12981.0
75	9806.0
76	6631.0
77	6631.0
78	6412.5
79	6194.0
80	4836.0
81	3478.0
82	3478.0
83	3632.0
84	3786.0
85	3786.0
86	2508.5
87	1231.0
88	1062.0
89	893.0
90	893.0
91	637.0
92	381.0
93	315.0
94	249.0
95	249.0
96	235.0
97	221.0
98	221.0
99	412.5
100	604.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11784115012962526
2	0.052479568101205784
3	0.010686216098711359
4	0.0029277304380031124
5	2.1957978285023342E-4
6	7.31932609500778E-5
7	0.0
8	4.3915956570046683E-4
9	1.463865219001556E-4
10	5.855460876006224E-4
11	0.0014638652190015562
12	0.0029277304380031124
13	0.014492265668115406
14	0.009441930662560036
15	0.025690834593477308
16	0.01134495544726206
17	0.021445625458372794
18	0.005928654136956302
19	0.007392519355957858
20	0.005269914788405602
21	0.007465712616907936
22	0.007538905877858014
23	0.01032024979396097
24	0.01427268588526517
25	0.016980836540418053
26	0.01939621415177062
27	0.009222350879709802
28	0.005343108049355679
29	0.012150081317712917
30	0.002708150655152879
31	0.006953359790257392
32	0.006441006963606846
33	0.008197645226408715
34	0.013101593710063928
35	0.016468483713767506
36	0.014492265668115406
37	0.010613022837761282
38	0.00666058674645708
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1366246.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.68540078734277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45646297445688	42.656520599283795
2	12.63838153358643	13.569931558678785
3	3.690175032064486	5.943255767154823
4	1.4898973101145505	3.199429369019343
5	0.7652308298604318	2.0540861897944094
6	0.46370056477817734	1.493637039926019
7	0.2791438834982812	1.049016588405838
8	0.19434639978499754	0.8346851491227794
9	0.14124490222818245	0.6824510266760118
>10	0.69834575639868	6.803679774395133
>50	0.0774135242063004	2.9749729205285114
>100	0.08854958853185486	10.420924435308734
>500	0.01245440595731002	4.5840130952292
>1k	0.0046532945335004475	3.7333964864765603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4013	0.29372455619266225	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2516	0.18415424455039575	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1891	0.13840845645659713	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1869	0.13679820471569543	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1835	0.13430963384339278	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1800	0.13174786971014005	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1753	0.12830778644548638	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1749	0.12801501340168608	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1698	0.1242821570932321	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1693	0.12391619078848172	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1616	0.11828030969532573	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1541	0.1127908151240699	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1475	0.10796005990136477	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1454	0.10642300142141314	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1410	0.10320249793960971	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1410	0.10320249793960971	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1379	0.1009335068501573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.463865219001556E-4
5	0.0	0.0	0.0	0.0	1.463865219001556E-4
6	0.0	0.0	0.0	0.0	1.463865219001556E-4
7	0.0	0.0	0.0	0.0	1.463865219001556E-4
8	0.0	0.0	0.0	0.0	1.463865219001556E-4
9	0.0	0.0	0.0	0.0	1.463865219001556E-4
10	0.0	0.0	0.0	0.0	1.463865219001556E-4
11	0.0	0.0	0.0	0.0	1.463865219001556E-4
12	0.0	0.0	0.0	0.0	2.1957978285023342E-4
13	0.0	0.0	0.0	0.0	2.1957978285023342E-4
14	0.0	0.0	0.0	0.0	2.1957978285023342E-4
15	0.0	0.0	0.0	0.0	2.927730438003112E-4
16	0.0	0.0	0.0	7.31932609500778E-5	2.927730438003112E-4
17	0.0	0.0	0.0	1.463865219001556E-4	2.927730438003112E-4
18	0.0	0.0	0.0	1.463865219001556E-4	2.927730438003112E-4
19	0.0	0.0	0.0	1.463865219001556E-4	2.927730438003112E-4
20	0.0	0.0	0.0	1.463865219001556E-4	3.65966304750389E-4
21	0.0	0.0	0.0	2.927730438003112E-4	3.65966304750389E-4
22	0.0	0.0	0.0	4.3915956570046683E-4	3.65966304750389E-4
23	0.0	0.0	0.0	0.0010247056533010893	3.65966304750389E-4
24	0.0	0.0	0.0	0.002195797828502334	3.65966304750389E-4
25	0.0	0.0	0.0	0.0023421843504024898	3.65966304750389E-4
26	0.0	0.0	0.0	0.00300092369895319	3.65966304750389E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAC	45	0.008853729	17.776863	3
CGCGGTC	265	0.0	17.511097	10
GTATTAA	120	1.6370905E-9	17.351494	1
GGTCCTA	295	0.0	16.272732	13
ACCGTCG	355	0.0	16.224518	8
TACCGTC	355	0.0	16.224518	7
ATACCGT	365	0.0	16.21597	6
GTACGGA	60	0.0024252108	16.016764	1
AACCGCG	290	0.0	15.999177	7
GAACCGC	295	0.0	15.725701	6
GTATAAG	155	1.8553692E-10	15.500095	1
GTGTAGG	125	5.0731614E-8	15.376094	1
TAAACGC	410	0.0	15.218729	28
CGTCGTA	385	0.0	14.9624605	10
CGTCTTA	150	1.9699655E-9	14.934205	15
GTCGTAA	130	8.3180566E-8	14.768472	30
TACGCAG	65	0.0041675908	14.766309	5
CGCCCCT	360	0.0	14.663764	5
GCGCCCC	350	0.0	14.627283	21
TGACTCG	230	0.0	14.605804	6
>>END_MODULE