FastQCFastQC Report
Thu 2 Feb 2017
SRR4062848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366246
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46580.3409342095054624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32460.23758532504395258No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20300.14858231972865796No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19470.14250727906980148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19210.14060425428509946No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18620.13628585188904485No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18500.13540753275764394No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18310.13401686079959246No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17410.12742946731408544No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17230.12611198861698406No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17000.12442854361513227No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16220.11871946926102621No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15730.11513299947447239No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15670.11469383990877191No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14360.10510552272431173No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14020.10261695185200907No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14000.10247056533010893No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13770.10078712032825714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2850.017.9667631
CGTCCCG450.00884958917.77830510
GTAATAC1051.188655E-716.7630183
ACCGTCG3850.016.2088688
TAGACAA805.6189136E-516.0010625
TCTAGAT3500.016.0004772
TATTAGC2100.016.0004772
TACCGTC4050.015.8035177
TCTAGCG4350.015.44760428
ATACCGT4150.015.42270856
GTTCAAA5850.015.3178171
ATTAGCT2200.015.273743
CGAGCCG5700.015.15723515
CTAGCGG4450.015.10046829
CGTCGTA4050.015.01279310
ATAGCAC1501.9699655E-914.9343243
GTTTTCG4950.014.86804128
TATAAGC650.004161290414.76967052
CGAACGA1757.4578566E-1114.62847116
ACGAACG1757.4578566E-1114.62793515
Produced by FastQC (version 0.11.5)