##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1366246
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217433756439178	32.0	32.0	32.0	32.0	32.0
2	31.314553162461227	32.0	32.0	32.0	32.0	32.0
3	31.382139819622527	32.0	32.0	32.0	32.0	32.0
4	31.47859316696993	32.0	32.0	32.0	32.0	32.0
5	31.398490462186167	32.0	32.0	32.0	32.0	32.0
6	34.92515183941984	36.0	36.0	36.0	36.0	36.0
7	34.945110909748315	36.0	36.0	36.0	36.0	36.0
8	34.88708256053449	36.0	36.0	36.0	32.0	36.0
9	34.99480108267472	36.0	36.0	36.0	36.0	36.0
10	34.83834390000044	36.0	36.0	36.0	32.0	36.0
11	35.00607284486103	36.0	36.0	36.0	36.0	36.0
12	34.91074813759747	36.0	36.0	36.0	32.0	36.0
13	34.95693674492002	36.0	36.0	36.0	36.0	36.0
14	34.90680375276487	36.0	36.0	36.0	32.0	36.0
15	34.87445086755972	36.0	36.0	36.0	32.0	36.0
16	34.88636526657717	36.0	36.0	36.0	32.0	36.0
17	34.85006946040464	36.0	36.0	36.0	32.0	36.0
18	34.851984928043706	36.0	36.0	36.0	32.0	36.0
19	34.839272722481894	36.0	36.0	36.0	32.0	36.0
20	34.82145748276665	36.0	36.0	36.0	32.0	36.0
21	34.8097209433733	36.0	36.0	36.0	32.0	36.0
22	34.79388704523197	36.0	36.0	36.0	32.0	36.0
23	34.74019686059465	36.0	36.0	36.0	32.0	36.0
24	34.72188683443538	36.0	36.0	36.0	32.0	36.0
25	34.69898246728627	36.0	36.0	36.0	32.0	36.0
26	34.62952206264465	36.0	36.0	36.0	32.0	36.0
27	34.61641022187805	36.0	36.0	36.0	32.0	36.0
28	34.58611113957516	36.0	36.0	36.0	32.0	36.0
29	34.54611907372465	36.0	36.0	36.0	32.0	36.0
30	34.53847842921407	36.0	36.0	36.0	32.0	36.0
31	34.53013293360054	36.0	36.0	36.0	32.0	36.0
32	34.490563924798316	36.0	36.0	36.0	32.0	36.0
33	34.451114221011444	36.0	36.0	36.0	32.0	36.0
34	34.441490039129114	36.0	36.0	36.0	32.0	36.0
35	34.401818559761566	36.0	36.0	36.0	32.0	36.0
36	34.371236219538794	36.0	36.0	36.0	32.0	36.0
37	34.368143072331044	36.0	36.0	36.0	32.0	36.0
38	33.93189440261856	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	31.0
22	109.0
23	320.0
24	889.0
25	2266.0
26	4729.0
27	9197.0
28	16241.0
29	25656.0
30	38935.0
31	56154.0
32	79256.0
33	126319.0
34	307832.0
35	698305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27212782591128	18.04813382250891	11.974675501497686	26.70506285008213
2	16.0405227169924	20.722549233446976	36.11853209451299	27.118395955047625
3	17.940326998212623	24.833668314490946	28.545957316617944	28.680047370678487
4	12.304198161232168	16.155202486237787	35.46160596849312	36.07899338403693
5	14.520382508261696	36.65082031407251	32.72114445066588	16.107652726999916
6	34.353650378190686	35.489686307403815	16.50198134737477	13.654681967030733
7	30.166888320745283	30.46412680613917	20.908286446847306	18.46069842626824
8	28.502332717033447	32.02241516361225	19.42684110442111	20.048411014933194
9	27.964183710366168	13.979786179708006	18.184020717409094	39.87200939251673
10	16.118140556872635	26.13704650071693	30.93621384869536	26.808599093715074
11	37.91794976148527	20.917196557072618	21.952358539939965	19.21249514150215
12	25.534915823905415	23.56798900342033	27.810938464094605	23.08615670857966
13	29.65683314486055	19.032384382010548	25.001445567961824	26.30933690516708
14	24.31320883150533	19.537271865587797	24.60612847622498	31.54339082668189
15	25.570065712909678	26.354551083772616	22.29605795735175	25.779325245965957
16	26.30765345893306	25.269040325856633	23.08319518095217	25.34011103425813
17	24.449769660807792	25.337823495915085	24.765379002024527	25.447027841252602
18	25.58141066835694	23.9404177578562	25.930176556784062	24.547995017002794
19	26.098960216534945	24.45328293733339	25.22371520209391	24.22404164403775
20	26.2950449626202	23.488888531055167	24.65727255560126	25.55879395072337
21	27.677779371766682	23.748100632097927	24.00471658064006	24.569403415495326
22	26.237314683676793	23.705191967765664	24.648214632075508	25.409278716482035
23	24.682981456473765	23.414541315796214	25.28236150909976	26.62011571863026
24	25.202928315984092	24.644463734934995	24.85328410842557	25.29932384065534
25	25.326442421705053	23.805484232684645	25.008490430699055	25.859582914911243
26	25.211876347214446	24.697034572862115	25.32503321146646	24.766055868456977
27	25.873964132374404	24.086072347146853	24.471800832353765	25.56816268812498
28	24.935772913516306	23.990042788780354	25.48340489194479	25.59077940575855
29	24.91213149022943	24.30857986043509	25.479964808680133	25.29932384065534
30	24.86316519865383	24.34729909547768	25.801429610772875	24.988106095095613
31	25.439488935374744	24.29760087129258	24.332660443287665	25.930249750045014
32	25.189899915534973	24.235898952311665	24.376722786379613	26.19747834577375
33	24.800218994236765	23.826675430339776	25.092406491949472	26.280699083473984
34	25.605344864687616	23.974013464632286	25.303935016095195	25.116706654584895
35	26.362236376172373	23.89313491128245	25.062470448220893	24.682158264324286
36	25.010576426207287	24.719706407191676	24.577418707904723	25.69229845869631
37	26.064705770410306	24.56987980202687	24.602816769454403	24.762597658108422
38	25.0995978027367	24.275221501268074	25.046971809594908	25.578208886400315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	181.0
1	160.5
2	140.0
3	140.0
4	537.0
5	934.0
6	934.0
7	1190.5
8	1447.0
9	1400.0
10	1353.0
11	1353.0
12	1792.0
13	2231.0
14	2700.0
15	3169.0
16	3169.0
17	4878.0
18	6587.0
19	6587.0
20	7529.5
21	8472.0
22	8639.5
23	8807.0
24	8807.0
25	9679.0
26	10551.0
27	10551.0
28	14004.0
29	17457.0
30	21565.0
31	25673.0
32	25673.0
33	33998.0
34	42323.0
35	42323.0
36	46884.5
37	51446.0
38	60008.0
39	68570.0
40	68570.0
41	74066.5
42	79563.0
43	91371.5
44	103180.0
45	103180.0
46	108057.0
47	112934.0
48	112934.0
49	121177.5
50	129421.0
51	130453.0
52	131485.0
53	131485.0
54	126261.5
55	121038.0
56	121038.0
57	118569.5
58	116101.0
59	104473.0
60	92845.0
61	92845.0
62	88214.5
63	83584.0
64	68937.0
65	54290.0
66	54290.0
67	45659.5
68	37029.0
69	37029.0
70	29661.0
71	22293.0
72	17516.0
73	12739.0
74	12739.0
75	9523.0
76	6307.0
77	6307.0
78	6136.5
79	5966.0
80	4663.5
81	3361.0
82	3361.0
83	3254.0
84	3147.0
85	3147.0
86	1991.5
87	836.0
88	729.0
89	622.0
90	622.0
91	358.5
92	95.0
93	68.5
94	42.0
95	42.0
96	28.5
97	15.0
98	15.0
99	13.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010100670011110736
2	0.0
3	0.0
4	3.6596630475038905E-4
5	7.31932609500778E-5
6	2.927730438003112E-4
7	2.1957978285023342E-4
8	0.0020494113066021787
9	0.0038060495694040458
10	6.587393485507002E-4
11	0.006075040658856458
12	2.1957978285023342E-4
13	7.31932609500778E-5
14	7.31932609500778E-5
15	0.0
16	7.31932609500778E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.463865219001556E-4
22	7.31932609500778E-5
23	7.31932609500778E-5
24	0.0
25	1.463865219001556E-4
26	7.31932609500778E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.31932609500778E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1366246.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.728508457904276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01220435115962	40.35454972598717
2	13.508547906565937	13.975540692776026
3	3.915790177045162	6.0767395587797735
4	1.6662839464930772	3.44777532877749
5	0.8063327704360468	2.0855195767693213
6	0.45659378539418727	1.417134929375385
7	0.3100063938809765	1.1225317857513548
8	0.22579437444395992	0.9344004966537268
9	0.16018185158396928	0.745737143801771
>10	0.7432310949009359	6.953714897589901
>50	0.07985161204141289	2.9328690038067307
>100	0.09641139941214405	10.919866860092606
>500	0.013651153921890018	4.9149777798976215
>1k	0.0051191827207087565	4.118642219941148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4658	0.3409342095054624	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3246	0.23758532504395258	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2030	0.14858231972865796	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1947	0.14250727906980148	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1921	0.14060425428509946	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1862	0.13628585188904485	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1850	0.13540753275764394	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1831	0.13401686079959246	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1741	0.12742946731408544	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1723	0.12611198861698406	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1700	0.12442854361513227	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1622	0.11871946926102621	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1573	0.11513299947447239	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1567	0.11469383990877191	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1436	0.10510552272431173	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1402	0.10261695185200907	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1400	0.10247056533010893	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1377	0.10078712032825714	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.927730438003112E-4
2	0.0	0.0	0.0	0.0	2.927730438003112E-4
3	0.0	0.0	0.0	0.0	2.927730438003112E-4
4	0.0	0.0	0.0	0.0	2.927730438003112E-4
5	0.0	0.0	0.0	0.0	2.927730438003112E-4
6	0.0	0.0	0.0	0.0	2.927730438003112E-4
7	0.0	0.0	0.0	0.0	2.927730438003112E-4
8	0.0	0.0	0.0	0.0	2.927730438003112E-4
9	0.0	0.0	0.0	0.0	2.927730438003112E-4
10	0.0	0.0	0.0	0.0	2.927730438003112E-4
11	0.0	0.0	0.0	0.0	2.927730438003112E-4
12	0.0	0.0	0.0	0.0	5.855460876006224E-4
13	0.0	0.0	0.0	0.0	6.587393485507002E-4
14	0.0	0.0	0.0	0.0	6.587393485507002E-4
15	0.0	0.0	0.0	0.0	6.587393485507002E-4
16	0.0	0.0	0.0	7.31932609500778E-5	7.31932609500778E-4
17	0.0	0.0	0.0	1.463865219001556E-4	7.31932609500778E-4
18	0.0	0.0	0.0	1.463865219001556E-4	8.051258704508559E-4
19	0.0	0.0	0.0	1.463865219001556E-4	8.051258704508559E-4
20	0.0	0.0	0.0	1.463865219001556E-4	8.051258704508559E-4
21	0.0	0.0	0.0	2.927730438003112E-4	8.051258704508559E-4
22	0.0	0.0	0.0	4.3915956570046683E-4	8.051258704508559E-4
23	0.0	0.0	0.0	8.783191314009337E-4	8.783191314009337E-4
24	0.0	0.0	0.0	0.0020494113066021787	8.783191314009337E-4
25	0.0	0.0	0.0	0.0022689910894524118	8.783191314009337E-4
26	0.0	0.0	0.0	0.0028545371770530344	8.783191314009337E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	285	0.0	17.966763	1
CGTCCCG	45	0.008849589	17.778305	10
GTAATAC	105	1.188655E-7	16.763018	3
ACCGTCG	385	0.0	16.208868	8
TAGACAA	80	5.6189136E-5	16.001062	5
TCTAGAT	350	0.0	16.000477	2
TATTAGC	210	0.0	16.000477	2
TACCGTC	405	0.0	15.803517	7
TCTAGCG	435	0.0	15.447604	28
ATACCGT	415	0.0	15.4227085	6
GTTCAAA	585	0.0	15.317817	1
ATTAGCT	220	0.0	15.27374	3
CGAGCCG	570	0.0	15.157235	15
CTAGCGG	445	0.0	15.100468	29
CGTCGTA	405	0.0	15.012793	10
ATAGCAC	150	1.9699655E-9	14.934324	3
GTTTTCG	495	0.0	14.868041	28
TATAAGC	65	0.0041612904	14.7696705	2
CGAACGA	175	7.4578566E-11	14.628471	16
ACGAACG	175	7.4578566E-11	14.627935	15
>>END_MODULE