FastQCFastQC Report
Thu 2 Feb 2017
SRR4062847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985141
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46440.4714046009657501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29540.29985555367201244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16350.16596609013329058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.15429263425235576No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15060.15287151788424194No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10690.10851238553668968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG453.5368415E-421.33282514
TGTCGGG659.433506E-619.6918423
GTAAGTT802.794639E-618.00048429
TAGGACG2500.017.9177534
GCCCTAA550.001355877517.4709841
GCGAAAT753.233592E-517.06626112
TCGGGTA753.233592E-517.06626125
TAGGACT1505.456968E-1217.0645284
TAGAGCG854.995658E-616.9364745
CTAAGGT1456.91216E-1116.549654
GTCCTAA2850.016.2960341
GGATATA703.6693315E-416.0150681
CTATCGA600.002441133416.00043131
TAGCGGC2100.016.00043130
CGAAATT805.620694E-515.99961913
GACGTGA1203.1091986E-815.9996197
TTGTCGG805.620694E-515.99961922
GGACCGT703.7021152E-415.9963716
TCTAGCG2050.015.61017628
TCGTTTA1850.015.56798830
Produced by FastQC (version 0.11.5)