##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062847_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	985141
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.133635692758702	32.0	32.0	32.0	32.0	32.0
2	30.740786344289802	32.0	32.0	32.0	32.0	32.0
3	30.802401889678737	32.0	32.0	32.0	32.0	32.0
4	30.85862734370004	32.0	32.0	32.0	32.0	32.0
5	30.78506426998775	32.0	32.0	32.0	32.0	32.0
6	34.41707329204652	36.0	36.0	36.0	32.0	36.0
7	34.32930819040117	36.0	36.0	36.0	32.0	36.0
8	34.28758928924895	36.0	36.0	36.0	32.0	36.0
9	34.42501733254428	36.0	36.0	36.0	32.0	36.0
10	34.15827988074803	36.0	36.0	36.0	32.0	36.0
11	34.45516530121069	36.0	36.0	36.0	32.0	36.0
12	34.25185633325585	36.0	36.0	36.0	32.0	36.0
13	34.35005648937563	36.0	36.0	36.0	32.0	36.0
14	34.25243188538494	36.0	36.0	36.0	32.0	36.0
15	34.20127372629908	36.0	36.0	36.0	32.0	36.0
16	34.22371112358535	36.0	36.0	36.0	32.0	36.0
17	34.14546344127389	36.0	36.0	36.0	32.0	36.0
18	34.18534808722812	36.0	36.0	36.0	32.0	36.0
19	34.16080134721832	36.0	36.0	36.0	32.0	36.0
20	34.11634882722372	36.0	36.0	36.0	32.0	36.0
21	34.098010335576326	36.0	36.0	36.0	32.0	36.0
22	34.074220847574104	36.0	36.0	36.0	32.0	36.0
23	34.03411897383217	36.0	36.0	36.0	32.0	36.0
24	34.03244814701652	36.0	36.0	36.0	32.0	36.0
25	34.00842214464731	36.0	36.0	36.0	32.0	36.0
26	33.974446297535074	36.0	36.0	36.0	32.0	36.0
27	33.98233349337811	36.0	36.0	36.0	32.0	36.0
28	33.96587696583535	36.0	36.0	36.0	32.0	36.0
29	33.93304207215008	36.0	36.0	36.0	32.0	36.0
30	33.900768519430216	36.0	36.0	36.0	32.0	36.0
31	33.91108683934584	36.0	36.0	36.0	32.0	36.0
32	33.861922303507825	36.0	36.0	36.0	32.0	36.0
33	33.808587806212515	36.0	36.0	36.0	32.0	36.0
34	33.810965130879744	36.0	36.0	36.0	32.0	36.0
35	33.77347303583954	36.0	36.0	36.0	32.0	36.0
36	33.71325525990696	36.0	36.0	36.0	27.0	36.0
37	33.71142303487521	36.0	36.0	36.0	27.0	36.0
38	33.11434403806156	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	1.0
9	5.0
10	9.0
11	10.0
12	5.0
13	2.0
14	161.0
15	291.0
16	330.0
17	443.0
18	638.0
19	799.0
20	1170.0
21	1529.0
22	2254.0
23	3363.0
24	4950.0
25	7600.0
26	10662.0
27	15059.0
28	20879.0
29	27809.0
30	37398.0
31	49684.0
32	66307.0
33	98002.0
34	211675.0
35	424103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.26483127283444	17.156867228667103	11.398750997220485	25.179550501277966
2	16.460162251534566	19.610437884148993	38.41093836960363	25.518461494712806
3	19.443699976853825	24.270692476681244	28.096815979793792	28.188791566671135
4	12.362578798306787	15.25403254458893	36.41136522824862	35.97202342885566
5	14.239020319995777	37.13134606522132	33.377557255937745	15.252076358845157
6	33.42563841244738	36.030448495085466	17.140220821630248	13.403692270836906
7	30.09369226708894	30.76415534847839	20.540430801713462	18.601721582719208
8	28.270382698040986	32.83959489898359	19.256407991984872	19.633614410990553
9	27.853023786491043	14.182465621602635	18.657570149999138	39.30694044190719
10	15.957203835007386	26.824749907373103	31.484415841483653	25.73363041613586
11	37.14420578203671	21.628126522657137	22.1872462238103	19.040421471495858
12	25.020682906427993	23.978162911259925	28.922976348906165	22.078177833405913
13	29.586275791773435	19.941746429456124	25.424769848650357	25.04720793012008
14	23.610727599061164	20.082473138364833	25.702300801585302	30.604498460988705
15	25.127855230413555	27.28634190372047	23.103087309105565	24.48271555676041
16	25.074236242311155	26.325465899042772	24.472326251661116	24.127971606984957
17	23.318970243631032	26.109213630991785	25.738325082340708	24.833491043036474
18	24.151575844262105	25.36405266112471	27.116073540414032	23.368297954199154
19	25.306703780742705	25.066822594513493	26.274248252156447	23.352225372587355
20	25.578730022089395	24.354575428794178	26.14578596023909	23.92090858887734
21	26.682536862675434	24.0240590817958	25.363043423089614	23.930360632439154
22	25.327412947899187	24.663805959164364	25.996501774998958	24.012279317937487
23	23.9239103069089	24.82467859434261	26.404804638961753	24.84660645978673
24	24.225842279133325	25.44319526987653	26.20816522945038	24.12279722153977
25	24.520139617898543	25.01954366308146	26.044748389297585	24.415568329722408
26	23.855806024868244	25.91123822403348	26.592591915739973	23.640363835358308
27	24.557462157012232	25.397921135528197	25.979613362475472	24.0650033449841
28	23.786344690086715	25.47412296641295	26.554946694792918	24.18458564870742
29	23.81815302337197	25.450597243118455	26.342451874366766	24.388797859142805
30	23.791628726625635	25.793441727649892	26.723696004596455	23.69123354112802
31	24.501842995652133	25.511201616922875	25.511506160407116	24.47544922701788
32	23.92097705670904	25.79554371670959	25.43861875317867	24.844860473402694
33	23.787030664059724	25.333633822033537	25.974405671927247	24.904929841979488
34	24.360775698410798	25.684603998692413	26.16114157856373	23.793478724333063
35	24.85324604916638	25.351732315920493	26.180478424986447	23.614543209926676
36	23.874155578612662	26.210819558291725	25.69416666497461	24.220858198121004
37	24.624282268478286	26.102991935418373	25.28577287604259	23.986952920060748
38	23.917172461609834	25.97699889247911	25.9602490348529	24.145579611058153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	120.0
1	156.0
2	192.0
3	192.0
4	470.0
5	748.0
6	748.0
7	1063.5
8	1379.0
9	1454.5
10	1530.0
11	1530.0
12	2018.5
13	2507.0
14	3099.0
15	3691.0
16	3691.0
17	5153.0
18	6615.0
19	6615.0
20	7737.0
21	8859.0
22	8691.0
23	8523.0
24	8523.0
25	9860.5
26	11198.0
27	11198.0
28	14503.0
29	17808.0
30	21904.0
31	26000.0
32	26000.0
33	31224.5
34	36449.0
35	36449.0
36	40489.5
37	44530.0
38	50586.5
39	56643.0
40	56643.0
41	60070.5
42	63498.0
43	70858.0
44	78218.0
45	78218.0
46	81871.0
47	85524.0
48	85524.0
49	88390.0
50	91256.0
51	90988.5
52	90721.0
53	90721.0
54	85464.0
55	80207.0
56	80207.0
57	76763.0
58	73319.0
59	65542.5
60	57766.0
61	57766.0
62	53192.5
63	48619.0
64	40861.5
65	33104.0
66	33104.0
67	27339.0
68	21574.0
69	21574.0
70	17375.0
71	13176.0
72	10389.5
73	7603.0
74	7603.0
75	5904.0
76	4205.0
77	4205.0
78	3949.0
79	3693.0
80	2855.5
81	2018.0
82	2018.0
83	1909.0
84	1800.0
85	1800.0
86	1234.5
87	669.0
88	556.5
89	444.0
90	444.0
91	320.0
92	196.0
93	175.5
94	155.0
95	155.0
96	150.0
97	145.0
98	145.0
99	292.0
100	439.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11734360868139687
2	0.050449631068040006
3	0.009846306264788492
4	0.003146757672251992
5	3.045249360243864E-4
6	2.0301662401625757E-4
7	1.0150831200812878E-4
8	4.0603324803251513E-4
9	2.0301662401625757E-4
10	6.090498720487728E-4
11	0.0021316745521707046
12	0.002842232736227606
13	0.013906638745113643
14	0.009440273016755976
15	0.02415897825793465
16	0.011165914320894166
17	0.021215237209698916
18	0.005075415600406439
19	0.007105581840569014
20	0.005379940536430826
21	0.0066995485925365
22	0.007105581840569014
23	0.009440273016755976
24	0.013703622121097384
25	0.01695188810535751
26	0.01928657928154447
27	0.008526698208682819
28	0.005988990408479598
29	0.01167345588093481
30	0.003451282608276379
31	0.006090498720487727
32	0.006090498720487727
33	0.008019156648642174
34	0.012079489128967325
35	0.016139821609292476
36	0.014921721865194932
37	0.009440273016755976
38	0.005887482096471469
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	985141.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.365957812493406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3352529177381	45.72112067027703
2	13.483678144052638	15.739755794260313
3	4.155473880515579	7.2761463960327175
4	1.6042426994619954	3.7453264687119745
5	0.7432431847379457	2.1690050182419087
6	0.41160457654243077	1.4414217209942837
7	0.2245413707915985	0.9173900522347314
8	0.16300970002961182	0.7611373819964422
9	0.10154084450616835	0.5333875782022778
>10	0.6221871517314659	6.9830089621450595
>50	0.07901779248071407	3.2229510413606026
>100	0.06869183706665227	7.65218679689526
>500	0.006467170064430515	2.477480712821446
>1k	0.001048730280718462	1.359681405825993
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4644	0.4714046009657501	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2954	0.29985555367201244	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1635	0.16596609013329058	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1520	0.15429263425235576	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1506	0.15287151788424194	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1069	0.10851238553668968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0150831200812878E-4
2	0.0	0.0	0.0	0.0	1.0150831200812878E-4
3	0.0	0.0	0.0	0.0	1.0150831200812878E-4
4	0.0	0.0	0.0	0.0	1.0150831200812878E-4
5	0.0	0.0	0.0	0.0	1.0150831200812878E-4
6	0.0	0.0	0.0	0.0	1.0150831200812878E-4
7	0.0	0.0	0.0	0.0	1.0150831200812878E-4
8	0.0	0.0	0.0	0.0	1.0150831200812878E-4
9	0.0	0.0	0.0	1.0150831200812878E-4	1.0150831200812878E-4
10	0.0	0.0	0.0	1.0150831200812878E-4	1.0150831200812878E-4
11	0.0	0.0	0.0	1.0150831200812878E-4	1.0150831200812878E-4
12	0.0	0.0	0.0	1.0150831200812878E-4	3.0452493602438634E-4
13	0.0	0.0	0.0	1.0150831200812878E-4	3.0452493602438634E-4
14	0.0	0.0	0.0	1.0150831200812878E-4	3.0452493602438634E-4
15	0.0	0.0	0.0	1.0150831200812878E-4	6.090498720487727E-4
16	0.0	0.0	0.0	3.0452493602438634E-4	6.090498720487727E-4
17	0.0	0.0	0.0	3.0452493602438634E-4	6.090498720487727E-4
18	0.0	0.0	0.0	4.0603324803251513E-4	6.090498720487727E-4
19	0.0	0.0	0.0	4.0603324803251513E-4	6.090498720487727E-4
20	0.0	0.0	0.0	4.0603324803251513E-4	7.105581840569015E-4
21	0.0	0.0	0.0	7.105581840569015E-4	7.105581840569015E-4
22	0.0	0.0	0.0	0.0012180997440975453	7.105581840569015E-4
23	0.0	0.0	0.0	0.001928657928154447	7.105581840569015E-4
24	0.0	0.0	0.0	0.0030452493602438637	7.105581840569015E-4
25	0.0	0.0	0.0	0.003451282608276379	7.105581840569015E-4
26	0.0	0.0	0.0	0.004060332480325151	7.105581840569015E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAG	45	3.5368415E-4	21.332825	14
TGTCGGG	65	9.433506E-6	19.69184	23
GTAAGTT	80	2.794639E-6	18.000484	29
TAGGACG	250	0.0	17.917753	4
GCCCTAA	55	0.0013558775	17.470984	1
GCGAAAT	75	3.233592E-5	17.066261	12
TCGGGTA	75	3.233592E-5	17.066261	25
TAGGACT	150	5.456968E-12	17.064528	4
TAGAGCG	85	4.995658E-6	16.936474	5
CTAAGGT	145	6.91216E-11	16.54965	4
GTCCTAA	285	0.0	16.296034	1
GGATATA	70	3.6693315E-4	16.015068	1
CTATCGA	60	0.0024411334	16.000431	31
TAGCGGC	210	0.0	16.000431	30
CGAAATT	80	5.620694E-5	15.999619	13
GACGTGA	120	3.1091986E-8	15.999619	7
TTGTCGG	80	5.620694E-5	15.999619	22
GGACCGT	70	3.7021152E-4	15.996371	6
TCTAGCG	205	0.0	15.610176	28
TCGTTTA	185	0.0	15.567988	30
>>END_MODULE