FastQCFastQC Report
Thu 2 Feb 2017
SRR4062847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985141
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT57200.5806275446864967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38650.3923296259114178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22870.23214950956259053No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.1787561374463148No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12880.13074270586646988No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10620.10780182735263277No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9970.1012037870721044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1650.021.33231728
TTATTCG507.197241E-419.1990922
CTAGCGG1850.019.02612129
TAGCGGC1900.018.52543430
GACGTGA1301.2732926E-1118.4644097
GTATATC1750.018.2848433
TCGATCG450.00885182817.77693216
GTCTAGG1101.061926E-817.4554861
CCGATAA854.9751015E-616.9438119
GTATAGC1354.4201443E-1016.5934871
CAAGACG2700.016.5918034
TAGGACG2850.016.2799264
CGCGTAA1407.403287E-1016.00248710
TCGCGTA1407.403287E-1016.0024879
AAGACGG2800.016.0016755
TATACTG1407.403287E-1016.0016755
GTGTATA2600.016.0008621
ACGTACC600.002441522216.0000513
ACGAACG1001.3145545E-615.9992415
CGATTTA600.002442349415.99923818
Produced by FastQC (version 0.11.5)