FastQCFastQC Report
Thu 2 Feb 2017
SRR4062846_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062846_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371553
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43450.3167941741952371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27000.1968571393157975No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15880.11578116193832831No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15570.11352095033877656No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15270.11133364879082325No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15260.1112607387392248No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15150.11045872817164193No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15070.10987544775885438No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14990.10929216734606682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14780.10776105626249952No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14730.1073965060045073No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14220.10367809337298668No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13880.1011991516186396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA550.001363775717.4565542
GAGCGTT550.001366444217.4514647
TAGTACT957.712224E-716.8385164
TAATACT2350.016.3369454
ATCGTTT2500.015.99950829
CTTATAC1451.2223609E-915.4466743
CTAGCGG3450.015.30387629
TAGCGGC3500.015.08525130
TCTTATA1603.0377123E-1015.0017272
GTAATAC1103.5472367E-614.5439463
GTATTAG2450.014.3821131
CATCGTT2950.014.10126128
TCTAGCG3650.014.02696628
TCTATAG800.001018919713.99854853
TCTATAC800.001018919713.99854853
GTCTTAA1851.7826096E-1013.8520591
CGGCATC3350.013.85082525
TGTAATA1402.0533116E-713.7158642
TTAGTAC1053.6873826E-513.7128653
TTTCGTT4800.013.66624628
Produced by FastQC (version 0.11.5)