##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062846_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1371553
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21794199713755	32.0	32.0	32.0	32.0	32.0
2	30.897835519298198	32.0	32.0	32.0	32.0	32.0
3	30.949414277100484	32.0	32.0	32.0	32.0	32.0
4	30.999131641285462	32.0	32.0	32.0	32.0	32.0
5	30.96815070216025	32.0	32.0	32.0	32.0	32.0
6	34.59024478091623	36.0	36.0	36.0	32.0	36.0
7	34.50675620993137	36.0	36.0	36.0	32.0	36.0
8	34.4975527741181	36.0	36.0	36.0	32.0	36.0
9	34.58860722115733	36.0	36.0	36.0	32.0	36.0
10	34.382475194177694	36.0	36.0	36.0	32.0	36.0
11	34.59153893433211	36.0	36.0	36.0	32.0	36.0
12	34.43913505347588	36.0	36.0	36.0	32.0	36.0
13	34.505590378206314	36.0	36.0	36.0	32.0	36.0
14	34.41487642110804	36.0	36.0	36.0	32.0	36.0
15	34.38344781426602	36.0	36.0	36.0	32.0	36.0
16	34.39348971567267	36.0	36.0	36.0	32.0	36.0
17	34.320868387878555	36.0	36.0	36.0	32.0	36.0
18	34.35587542005303	36.0	36.0	36.0	32.0	36.0
19	34.3360643008327	36.0	36.0	36.0	32.0	36.0
20	34.30829286217886	36.0	36.0	36.0	32.0	36.0
21	34.289559353521156	36.0	36.0	36.0	32.0	36.0
22	34.26709795392522	36.0	36.0	36.0	32.0	36.0
23	34.229104526037275	36.0	36.0	36.0	32.0	36.0
24	34.2203298013274	36.0	36.0	36.0	32.0	36.0
25	34.192676476957146	36.0	36.0	36.0	32.0	36.0
26	34.14997889254006	36.0	36.0	36.0	32.0	36.0
27	34.15915826803631	36.0	36.0	36.0	32.0	36.0
28	34.140728794293764	36.0	36.0	36.0	32.0	36.0
29	34.10762106896343	36.0	36.0	36.0	32.0	36.0
30	34.085379857723325	36.0	36.0	36.0	32.0	36.0
31	34.099622836303084	36.0	36.0	36.0	32.0	36.0
32	34.045260372730766	36.0	36.0	36.0	32.0	36.0
33	34.02137722712867	36.0	36.0	36.0	32.0	36.0
34	34.02355577947043	36.0	36.0	36.0	32.0	36.0
35	33.96765710111093	36.0	36.0	36.0	32.0	36.0
36	33.93632327733599	36.0	36.0	36.0	32.0	36.0
37	33.933554153576274	36.0	36.0	36.0	32.0	36.0
38	33.345123374743814	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	6.0
9	4.0
10	14.0
11	19.0
12	5.0
13	12.0
14	247.0
15	473.0
16	591.0
17	730.0
18	876.0
19	1065.0
20	1473.0
21	1894.0
22	2791.0
23	3894.0
24	5726.0
25	8790.0
26	12542.0
27	17958.0
28	25411.0
29	34088.0
30	46034.0
31	61811.0
32	84995.0
33	126550.0
34	286603.0
35	646948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32395369649862	17.463964996445043	11.588795825735556	25.623285481320778
2	16.575969210523628	19.93817010952265	37.75679173189346	25.72906894806027
3	18.791157428371218	24.25567535917387	28.541261518246536	28.41190569420837
4	12.6132497670478	15.607287065648045	36.23824849109678	35.541214676207375
5	14.68513725347235	36.53144252852612	33.35751522000656	15.42590499799497
6	33.63143888301557	35.60285808027414	17.188655171156718	13.57704786555357
7	30.097874524626118	30.653959893609574	20.972154172791114	18.27601140897319
8	28.095740135584222	32.518632258779505	19.772286164663814	19.61334144097245
9	27.78646625093234	14.484863464593682	18.789995836823913	38.938674447650065
10	16.130847962809852	26.401812261491774	31.308319225166347	26.159020550532023
11	37.04008043648019	21.334843097096133	22.591043348350766	19.03403311807291
12	25.344547250840314	23.789983303074713	28.47481972424554	22.39064972183943
13	29.459935217310772	19.62301491810284	25.247456163762237	25.66959370082415
14	23.80735018641249	20.051683618669976	25.268097834290128	30.872868360627407
15	25.039799946471536	27.278733811102857	22.634300662250688	25.047165580174923
16	25.576124905571223	25.820327197528957	24.005909272541135	24.597638624358687
17	23.8319048532347	25.97539071416226	25.409784061647915	24.78292037095512
18	24.862118145281247	24.625947163031622	26.646707460057286	23.86522723162985
19	25.548620428163098	24.979291839234673	25.79274339380505	23.679344338797176
20	25.839154949911375	24.171381197825106	25.3400550796263	24.649408772637223
21	26.883837323253534	24.177151873629192	24.648986186942928	24.290024616174342
22	25.81300457324187	24.368918871622565	25.158226002146623	24.659850552988942
23	24.048519230138215	24.306792619301962	25.871745605815892	25.77294254474393
24	24.645696105519303	25.07235565511674	25.59053079773158	24.691417441632378
25	24.696386093921596	24.613472917216626	25.62921402392158	25.060926964940194
26	24.373416175339187	25.49841936577735	26.01253568732977	24.115628771553688
27	25.19913549963979	24.904770793654496	25.102520876050367	24.793572830655346
28	24.259932641274318	24.91106212854448	25.900516455784807	24.928488774396392
29	24.379811243571336	25.21574715417928	25.729165223491083	24.675276378758305
30	24.190136762967764	25.29229010344171	26.047960731959314	24.46961240163121
31	24.98166195880904	25.165698602808966	24.717492085111843	25.13514735327015
32	24.450875565822017	25.17412151756965	24.791535419291613	25.583467497316718
33	24.062092806710655	24.810070648833225	25.71649612703811	25.41134041741801
34	25.003226679131007	24.92892190998987	25.57440357479588	24.49344783608324
35	25.570045043566907	24.865624036069857	25.5467101861757	24.017620734187535
36	24.344549078059075	25.545777652029468	25.191964682873614	24.917708587037847
37	25.230017398050776	25.308038832799333	24.989025957075086	24.4729178120748
38	24.370162104145642	25.30500253743445	25.5324237457199	24.792411612700004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	756.0
1	567.0
2	378.0
3	378.0
4	809.5
5	1241.0
6	1241.0
7	1434.0
8	1627.0
9	1526.0
10	1425.0
11	1425.0
12	1844.5
13	2264.0
14	2943.5
15	3623.0
16	3623.0
17	5228.5
18	6834.0
19	6834.0
20	8434.5
21	10035.0
22	10361.0
23	10687.0
24	10687.0
25	12428.0
26	14169.0
27	14169.0
28	18377.5
29	22586.0
30	27701.0
31	32816.0
32	32816.0
33	41591.5
34	50367.0
35	50367.0
36	55517.0
37	60667.0
38	68328.0
39	75989.0
40	75989.0
41	81218.0
42	86447.0
43	96372.0
44	106297.0
45	106297.0
46	109545.5
47	112794.0
48	112794.0
49	119475.5
50	126157.0
51	127318.0
52	128479.0
53	128479.0
54	122245.5
55	116012.0
56	116012.0
57	111730.0
58	107448.0
59	95858.0
60	84268.0
61	84268.0
62	79526.0
63	74784.0
64	61774.0
65	48764.0
66	48764.0
67	40548.0
68	32332.0
69	32332.0
70	26226.5
71	20121.0
72	15585.0
73	11049.0
74	11049.0
75	8498.0
76	5947.0
77	5947.0
78	5841.0
79	5735.0
80	4435.5
81	3136.0
82	3136.0
83	3196.0
84	3256.0
85	3256.0
86	2130.5
87	1005.0
88	869.0
89	733.0
90	733.0
91	526.5
92	320.0
93	280.0
94	240.0
95	240.0
96	204.5
97	169.0
98	169.0
99	382.5
100	596.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11920793436345516
2	0.05067248586091824
3	0.01035322732697898
4	0.001968571393157975
5	2.1873015479533054E-4
6	2.1873015479533054E-4
7	7.291005159844351E-5
8	5.103703611891046E-4
9	2.9164020639377405E-4
10	5.103703611891046E-4
11	0.0026247618575439666
12	0.0031351322187330713
13	0.014509100268090259
14	0.009624126810994544
15	0.023331216511501926
16	0.010863597688168084
17	0.020560634550761073
18	0.006488994592261473
19	0.008238835830624118
20	0.006124444334269255
21	0.007072275005049021
22	0.007072275005049021
23	0.010061587120585205
24	0.01472783042288559
25	0.017425502332028
26	0.01953989382838286
27	0.00911375644980544
28	0.006561904643859916
29	0.011373968049357188
30	0.0040829628895128365
31	0.006343174489064587
32	0.007072275005049021
33	0.008895026295010109
34	0.012686348978129173
35	0.015602751042066912
36	0.01509238068087781
37	0.009842856965789874
38	0.006488994592261473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1371553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.258421383793745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30757692094458	47.368510779556125
2	11.96789901799692	13.944618081383117
3	3.222176742429503	5.631567913005534
4	1.2628675053610774	2.9429066911690422
5	0.6143642119205878	1.789594457059598
6	0.3614465970514877	1.2634384895258322
7	0.2340508951821702	0.9544804973743909
8	0.155535046428229	0.7248981019810952
9	0.11785319065026384	0.6179346758094888
>10	0.6075196861217583	6.462652002647712
>50	0.06273632980849587	2.5914800642340046
>100	0.07379948781151792	9.334673438828814
>500	0.008785626603522789	3.484803862658945
>1k	0.0033887416899302187	2.8884409447662835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4345	0.3167941741952371	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2700	0.1968571393157975	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1588	0.11578116193832831	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1557	0.11352095033877656	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1527	0.11133364879082325	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1526	0.1112607387392248	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1515	0.11045872817164193	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1507	0.10987544775885438	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1499	0.10929216734606682	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1478	0.10776105626249952	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1473	0.1073965060045073	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1422	0.10367809337298668	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1388	0.1011991516186396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.291005159844351E-5
2	0.0	0.0	0.0	0.0	7.291005159844351E-5
3	0.0	0.0	0.0	0.0	7.291005159844351E-5
4	0.0	0.0	0.0	0.0	7.291005159844351E-5
5	0.0	0.0	0.0	0.0	7.291005159844351E-5
6	0.0	0.0	0.0	0.0	7.291005159844351E-5
7	0.0	0.0	0.0	0.0	7.291005159844351E-5
8	0.0	0.0	0.0	0.0	1.4582010319688703E-4
9	0.0	0.0	0.0	0.0	1.4582010319688703E-4
10	0.0	0.0	0.0	2.1873015479533054E-4	1.4582010319688703E-4
11	0.0	0.0	0.0	2.1873015479533054E-4	1.4582010319688703E-4
12	0.0	0.0	0.0	2.1873015479533054E-4	2.1873015479533054E-4
13	0.0	0.0	0.0	2.1873015479533054E-4	2.1873015479533054E-4
14	0.0	0.0	0.0	2.1873015479533054E-4	2.1873015479533054E-4
15	0.0	0.0	0.0	2.1873015479533054E-4	2.1873015479533054E-4
16	0.0	0.0	0.0	2.9164020639377405E-4	3.645502579922176E-4
17	0.0	0.0	0.0	2.9164020639377405E-4	3.645502579922176E-4
18	0.0	7.291005159844351E-5	0.0	3.645502579922176E-4	3.645502579922176E-4
19	0.0	7.291005159844351E-5	0.0	4.374603095906611E-4	3.645502579922176E-4
20	0.0	7.291005159844351E-5	0.0	5.103703611891046E-4	3.645502579922176E-4
21	0.0	7.291005159844351E-5	0.0	8.749206191813222E-4	4.374603095906611E-4
22	0.0	7.291005159844351E-5	0.0	0.0013123809287719833	4.374603095906611E-4
23	0.0	7.291005159844351E-5	0.0	0.0023331216511501924	5.832804127875481E-4
24	0.0	7.291005159844351E-5	0.0	0.0033538623735284018	5.832804127875481E-4
25	0.0	7.291005159844351E-5	0.0	0.003645502579922176	5.832804127875481E-4
26	0.0	7.291005159844351E-5	0.0	0.004666243302300385	5.832804127875481E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACGA	55	0.0013637757	17.456554	2
GAGCGTT	55	0.0013664442	17.451464	7
TAGTACT	95	7.712224E-7	16.838516	4
TAATACT	235	0.0	16.336945	4
ATCGTTT	250	0.0	15.999508	29
CTTATAC	145	1.2223609E-9	15.446674	3
CTAGCGG	345	0.0	15.303876	29
TAGCGGC	350	0.0	15.085251	30
TCTTATA	160	3.0377123E-10	15.001727	2
GTAATAC	110	3.5472367E-6	14.543946	3
GTATTAG	245	0.0	14.382113	1
CATCGTT	295	0.0	14.101261	28
TCTAGCG	365	0.0	14.026966	28
TCTATAG	80	0.0010189197	13.9985485	3
TCTATAC	80	0.0010189197	13.9985485	3
GTCTTAA	185	1.7826096E-10	13.852059	1
CGGCATC	335	0.0	13.850825	25
TGTAATA	140	2.0533116E-7	13.715864	2
TTAGTAC	105	3.6873826E-5	13.712865	3
TTTCGTT	480	0.0	13.666246	28
>>END_MODULE