FastQCFastQC Report
Thu 2 Feb 2017
SRR4062846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371553
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50930.37133089279087284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35900.2617470852384122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19370.14122676994618508No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16950.12358253745936176No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16490.12022867508583335No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16360.11928084441505359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.11833301374427382No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16230.11833301374427382No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15590.11366677044197344No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15470.11279184982279213No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15040.10965671760405904No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14940.10892761708807461No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14100.10280317275380536No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13830.10083460136064738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG2850.017.965718
TACCGTC2800.017.1442437
CGTCGTA2950.016.81430610
ATCGTTT2900.016.55125229
ATACCGT3300.016.4861836
ATTAGGC805.6217483E-516.0001283
CCGTCGT3050.015.7384039
TAGCGTA2600.015.385867
GAACCGC2350.014.9799356
TCGTTTA3100.014.96731530
CATCGTT3250.014.76881128
ACGAACG1851.0913936E-1114.70228515
GTAGGAC5050.014.5743743
TCTAGCG3250.014.27651828
TAATACT2250.014.2228544
TCCCCGC3300.014.062771
GGAGCGT1155.61098E-613.9141686
CGCCCCT3350.013.8518685
GTATTAG2900.013.7952251
AGCGTAT2900.013.79371558
Produced by FastQC (version 0.11.5)