##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1371553
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197794033478836	32.0	32.0	32.0	32.0	32.0
2	31.29045760535685	32.0	32.0	32.0	32.0	32.0
3	31.36556297860892	32.0	32.0	32.0	32.0	32.0
4	31.467955667772227	32.0	32.0	32.0	32.0	32.0
5	31.39252584479054	32.0	32.0	32.0	32.0	32.0
6	34.9005907901481	36.0	36.0	36.0	36.0	36.0
7	34.93596601808315	36.0	36.0	36.0	36.0	36.0
8	34.875092686903095	36.0	36.0	36.0	32.0	36.0
9	34.982143599263026	36.0	36.0	36.0	36.0	36.0
10	34.82436041479986	36.0	36.0	36.0	32.0	36.0
11	34.99612045615444	36.0	36.0	36.0	36.0	36.0
12	34.89416449820022	36.0	36.0	36.0	32.0	36.0
13	34.94846863373125	36.0	36.0	36.0	36.0	36.0
14	34.89435770983695	36.0	36.0	36.0	32.0	36.0
15	34.86408618551379	36.0	36.0	36.0	32.0	36.0
16	34.87822563182028	36.0	36.0	36.0	32.0	36.0
17	34.840306572184964	36.0	36.0	36.0	32.0	36.0
18	34.83861651718891	36.0	36.0	36.0	32.0	36.0
19	34.838319773278904	36.0	36.0	36.0	32.0	36.0
20	34.813767313403126	36.0	36.0	36.0	32.0	36.0
21	34.8097062235291	36.0	36.0	36.0	32.0	36.0
22	34.794823094696305	36.0	36.0	36.0	32.0	36.0
23	34.73410579102667	36.0	36.0	36.0	32.0	36.0
24	34.71473359031696	36.0	36.0	36.0	32.0	36.0
25	34.69855995357088	36.0	36.0	36.0	32.0	36.0
26	34.63351106373578	36.0	36.0	36.0	32.0	36.0
27	34.61829181956512	36.0	36.0	36.0	32.0	36.0
28	34.580764286906884	36.0	36.0	36.0	32.0	36.0
29	34.550629104380214	36.0	36.0	36.0	32.0	36.0
30	34.52956976507652	36.0	36.0	36.0	32.0	36.0
31	34.52303556625227	36.0	36.0	36.0	32.0	36.0
32	34.48885168856034	36.0	36.0	36.0	32.0	36.0
33	34.4549047685361	36.0	36.0	36.0	32.0	36.0
34	34.44152577406779	36.0	36.0	36.0	32.0	36.0
35	34.402803245663854	36.0	36.0	36.0	32.0	36.0
36	34.38224771481671	36.0	36.0	36.0	32.0	36.0
37	34.372947308634814	36.0	36.0	36.0	32.0	36.0
38	33.93798635561294	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	8.0
21	31.0
22	89.0
23	330.0
24	852.0
25	2231.0
26	4817.0
27	9336.0
28	16162.0
29	25770.0
30	38965.0
31	56747.0
32	81023.0
33	128681.0
34	309972.0
35	696537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59499882604974	18.68914234547971	12.345290126130383	26.37056870234017
2	15.59582458716506	20.965868617545222	37.002799016880864	26.435507778408855
3	17.77357491835897	24.965932778390627	28.91262678146597	28.34786552178443
4	12.085141503931313	16.501744009705792	36.153714915657595	35.259399570705305
5	14.129895089726755	37.08044822183321	33.070395383918815	15.719261304521225
6	33.5678856533744	36.127254658783606	16.809169778112192	13.495689909729801
7	29.778782644707036	30.916038604583	21.03369406954337	18.271484681166594
8	27.985583919785583	32.78878094732575	19.553431073754453	19.67220405913422
9	27.683854329580527	14.241341823264483	18.632424041583455	39.442379805571534
10	15.88694760127477	26.683927057229788	31.355387844767307	26.073737496728132
11	37.17112266712554	21.321575752979985	22.453561251899405	19.05374032799508
12	25.200029747452874	23.87911160106916	28.46080262710839	22.460056024369578
13	29.51347016149612	19.54460282162517	25.30910283985272	25.63282417702599
14	23.685705182373557	20.045670856321266	25.283601873205047	30.985022088100134
15	24.99203457686287	27.31020966743538	22.610209011244915	25.08754674445683
16	25.54060980508956	25.89429646539361	23.86214750724179	24.702946222275042
17	23.97967851041848	25.979382495608995	25.27733160876758	24.763607385204946
18	24.8360070664422	24.621359874536385	26.667507562595098	23.875125496426314
19	25.563357741188277	24.796489818475845	25.920981544278636	23.719170896057243
20	25.866590645786204	24.091522529570494	25.394206421479886	24.64768040316342
21	26.85709327827162	24.22430939548774	24.72279578171298	24.195801544527658
22	25.721572553156896	24.426398396562146	25.32705626395772	24.52497278632324
23	24.051693339652218	24.139112682731216	26.04177755094601	25.767416426670554
24	24.655335958581258	25.045988015045715	25.645600279391317	24.653075746981706
25	24.739947694252713	24.51720716181899	25.770532776396944	24.97231236753136
26	24.496902416457843	25.34440885623815	26.047115933543946	24.111572793760068
27	25.170226742969465	24.858900822644113	25.211785472380576	24.759086962005846
28	24.255861785873385	24.67618823333841	26.187394872819354	24.88055510796885
29	24.420784322589064	24.93246706470694	25.88357868780864	24.763169924895358
30	24.231145278381515	25.061372035932987	26.21925656536787	24.488226120317623
31	24.978254577110764	24.83505923577142	25.059330554488234	25.127355632629584
32	24.55187659536307	24.928529921920624	25.078870448316614	25.440723034399692
33	24.133299989136404	24.63098400134738	25.852591915879298	25.383124093636923
34	24.967390979422596	24.657741990284006	25.893129904568035	24.481737125725363
35	25.651870543828785	24.6627727838443	25.635830332477127	24.04952633984979
36	24.552824426033844	25.258229175248786	25.3839261042045	24.80502029451286
37	25.372625046206743	25.05801817355946	25.117585685715387	24.451771094518403
38	24.424739273465388	24.98264739506778	25.70919658897366	24.883416742493175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	390.0
1	302.0
2	214.0
3	214.0
4	690.0
5	1166.0
6	1166.0
7	1400.0
8	1634.0
9	1571.0
10	1508.0
11	1508.0
12	1966.0
13	2424.0
14	3141.0
15	3858.0
16	3858.0
17	5758.0
18	7658.0
19	7658.0
20	9039.5
21	10421.0
22	10700.0
23	10979.0
24	10979.0
25	12529.0
26	14079.0
27	14079.0
28	18163.0
29	22247.0
30	27577.5
31	32908.0
32	32908.0
33	41819.5
34	50731.0
35	50731.0
36	55983.5
37	61236.0
38	68790.5
39	76345.0
40	76345.0
41	81818.0
42	87291.0
43	96514.5
44	105738.0
45	105738.0
46	109335.0
47	112932.0
48	112932.0
49	119677.0
50	126422.0
51	127299.0
52	128176.0
53	128176.0
54	121981.5
55	115787.0
56	115787.0
57	111526.0
58	107265.0
59	95679.5
60	84094.0
61	84094.0
62	79569.0
63	75044.0
64	61658.5
65	48273.0
66	48273.0
67	40533.5
68	32794.0
69	32794.0
70	26538.5
71	20283.0
72	15749.0
73	11215.0
74	11215.0
75	8525.0
76	5835.0
77	5835.0
78	5681.5
79	5528.0
80	4238.5
81	2949.0
82	2949.0
83	2885.5
84	2822.0
85	2822.0
86	1755.0
87	688.0
88	580.0
89	472.0
90	472.0
91	276.0
92	80.0
93	59.0
94	38.0
95	38.0
96	28.5
97	19.0
98	19.0
99	14.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008384655933821004
2	0.0
3	0.0
4	7.291005159844351E-5
5	0.0
6	2.9164020639377405E-4
7	4.374603095906611E-4
8	0.002114391496354862
9	0.0033538623735284018
10	8.749206191813222E-4
11	0.005030793560292603
12	5.103703611891046E-4
13	2.1873015479533054E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	7.291005159844351E-5
22	0.0
23	2.1873015479533054E-4
24	0.0
25	1.4582010319688703E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.291005159844351E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1371553.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.54390136812908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.17268006294367	45.33276113897655
2	12.673099204800872	14.331729429295512
3	3.4047679499032117	5.7755658942208274
4	1.3341592733043572	3.0175428143638534
5	0.6748320564416117	1.9078818619743105
6	0.3766242727114182	1.2777483437422668
7	0.2601209209817796	1.0295776189846448
8	0.1728656002646442	0.7819596361045176
9	0.136307622460804	0.6936628284133088
>10	0.6365044191903019	6.560399091561264
>50	0.06431283179730848	2.5585248108418055
>100	0.0793562636501785	9.446565283468491
>500	0.011003687673451735	4.2388072184716545
>1k	0.0032363787274858045	2.6744715642277206
>5k	1.2945514909943215E-4	0.37280246535325234
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5093	0.37133089279087284	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3590	0.2617470852384122	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1937	0.14122676994618508	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1695	0.12358253745936176	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1649	0.12022867508583335	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1636	0.11928084441505359	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1623	0.11833301374427382	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1623	0.11833301374427382	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1559	0.11366677044197344	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1547	0.11279184982279213	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1504	0.10965671760405904	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1494	0.10892761708807461	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1410	0.10280317275380536	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1383	0.10083460136064738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	7.291005159844351E-5	0.0	0.0	0.0
4	0.0	7.291005159844351E-5	0.0	0.0	0.0
5	0.0	7.291005159844351E-5	0.0	0.0	7.291005159844351E-5
6	0.0	7.291005159844351E-5	0.0	0.0	1.4582010319688703E-4
7	0.0	7.291005159844351E-5	0.0	0.0	1.4582010319688703E-4
8	0.0	7.291005159844351E-5	0.0	0.0	1.4582010319688703E-4
9	0.0	7.291005159844351E-5	0.0	0.0	1.4582010319688703E-4
10	0.0	7.291005159844351E-5	0.0	2.1873015479533054E-4	1.4582010319688703E-4
11	0.0	7.291005159844351E-5	0.0	2.1873015479533054E-4	1.4582010319688703E-4
12	0.0	7.291005159844351E-5	0.0	2.1873015479533054E-4	2.1873015479533054E-4
13	0.0	7.291005159844351E-5	0.0	2.1873015479533054E-4	2.9164020639377405E-4
14	0.0	7.291005159844351E-5	0.0	2.1873015479533054E-4	4.374603095906611E-4
15	0.0	7.291005159844351E-5	0.0	2.1873015479533054E-4	4.374603095906611E-4
16	0.0	7.291005159844351E-5	0.0	2.9164020639377405E-4	4.374603095906611E-4
17	0.0	7.291005159844351E-5	0.0	2.9164020639377405E-4	4.374603095906611E-4
18	0.0	7.291005159844351E-5	0.0	2.9164020639377405E-4	4.374603095906611E-4
19	0.0	7.291005159844351E-5	0.0	3.645502579922176E-4	5.103703611891046E-4
20	0.0	1.4582010319688703E-4	0.0	4.374603095906611E-4	5.103703611891046E-4
21	0.0	1.4582010319688703E-4	0.0	9.478306707797657E-4	5.103703611891046E-4
22	0.0	1.4582010319688703E-4	0.0	0.0014582010319688704	5.103703611891046E-4
23	0.0	1.4582010319688703E-4	0.0	0.002406031702748636	5.103703611891046E-4
24	0.0	1.4582010319688703E-4	0.0	0.0034996824767252886	5.103703611891046E-4
25	0.0	1.4582010319688703E-4	0.0	0.003718412631520619	5.103703611891046E-4
26	0.0	1.4582010319688703E-4	0.0	0.004739153353898829	5.832804127875481E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	285	0.0	17.96571	8
TACCGTC	280	0.0	17.144243	7
CGTCGTA	295	0.0	16.814306	10
ATCGTTT	290	0.0	16.551252	29
ATACCGT	330	0.0	16.486183	6
ATTAGGC	80	5.6217483E-5	16.000128	3
CCGTCGT	305	0.0	15.738403	9
TAGCGTA	260	0.0	15.38586	7
GAACCGC	235	0.0	14.979935	6
TCGTTTA	310	0.0	14.967315	30
CATCGTT	325	0.0	14.768811	28
ACGAACG	185	1.0913936E-11	14.702285	15
GTAGGAC	505	0.0	14.574374	3
TCTAGCG	325	0.0	14.276518	28
TAATACT	225	0.0	14.222854	4
TCCCCGC	330	0.0	14.06277	1
GGAGCGT	115	5.61098E-6	13.914168	6
CGCCCCT	335	0.0	13.851868	5
GTATTAG	290	0.0	13.795225	1
AGCGTAT	290	0.0	13.7937155	8
>>END_MODULE