FastQCFastQC Report
Thu 2 Feb 2017
SRR4062845_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062845_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021279
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38990.1928976652901455No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27880.13793246751190705No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27080.133974577482871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24670.12205143377039983No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24420.12081459313632605No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24360.12051775138414834No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24160.11952827887688933No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23580.11665880860583819No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23310.1153230207210385No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23000.11378933833478705No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21570.10671460990788505No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21100.10438934951582637No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20550.10166830012086406No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20270.10028303861070144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT659.452549E-619.6891884
CTAGCGG6400.017.74938229
TCTAGCG6450.017.61135528
ACGAATT652.1019549E-417.2301721
TAGCGGC7000.016.45657230
CGCAAGA6950.016.1209282
CAAGACG7250.016.1077924
AAGACGG7300.015.9974655
GTATCAA31100.015.8600181
GCGCAAG7150.015.6785311
GACGGAC7100.015.3230317
ACGATTA951.4339335E-515.15736932
AGACGGA7650.015.05643756
TCTAGAT4650.014.80104352
GTACGAA650.004163993514.76871719
TACGTTG650.004167418514.7668915
GTATTAG4300.014.5247791
CATCGTT4200.014.47532828
ATCGTTT4150.014.26456429
GTTATTC3400.014.1168063
Produced by FastQC (version 0.11.5)