##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062845_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2021279
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224176375453364	32.0	32.0	32.0	32.0	32.0
2	30.798746734122307	32.0	32.0	32.0	32.0	32.0
3	30.840840378789864	32.0	32.0	32.0	32.0	32.0
4	30.926851760692116	32.0	32.0	32.0	32.0	32.0
5	30.813854495099392	32.0	32.0	32.0	32.0	32.0
6	34.46066723099582	36.0	36.0	36.0	32.0	36.0
7	34.38959144185439	36.0	36.0	36.0	32.0	36.0
8	34.376811909686886	36.0	36.0	36.0	32.0	36.0
9	34.532775039962324	36.0	36.0	36.0	32.0	36.0
10	34.228039770857954	36.0	36.0	36.0	32.0	36.0
11	34.500872467383275	36.0	36.0	36.0	32.0	36.0
12	34.313236816886736	36.0	36.0	36.0	32.0	36.0
13	34.40700417903714	36.0	36.0	36.0	32.0	36.0
14	34.29497263861149	36.0	36.0	36.0	32.0	36.0
15	34.23904814723747	36.0	36.0	36.0	32.0	36.0
16	34.2543943710888	36.0	36.0	36.0	32.0	36.0
17	34.17731545224583	36.0	36.0	36.0	32.0	36.0
18	34.20218633845204	36.0	36.0	36.0	32.0	36.0
19	34.19221591873264	36.0	36.0	36.0	32.0	36.0
20	34.18179034165991	36.0	36.0	36.0	32.0	36.0
21	34.16854476794148	36.0	36.0	36.0	32.0	36.0
22	34.145364890250185	36.0	36.0	36.0	32.0	36.0
23	34.081651271299016	36.0	36.0	36.0	32.0	36.0
24	34.07042867412168	36.0	36.0	36.0	32.0	36.0
25	34.03848949105987	36.0	36.0	36.0	32.0	36.0
26	33.98901190780689	36.0	36.0	36.0	32.0	36.0
27	33.99540291073127	36.0	36.0	36.0	32.0	36.0
28	33.98039756015869	36.0	36.0	36.0	32.0	36.0
29	33.9474797887872	36.0	36.0	36.0	32.0	36.0
30	33.92485203675495	36.0	36.0	36.0	32.0	36.0
31	33.9476984622113	36.0	36.0	36.0	32.0	36.0
32	33.9012170017103	36.0	36.0	36.0	32.0	36.0
33	33.86215905869501	36.0	36.0	36.0	32.0	36.0
34	33.881576961913716	36.0	36.0	36.0	32.0	36.0
35	33.83611812124897	36.0	36.0	36.0	32.0	36.0
36	33.78687454824396	36.0	36.0	36.0	32.0	36.0
37	33.802875802895095	36.0	36.0	36.0	32.0	36.0
38	33.20390901008718	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	8.0
8	2.0
9	7.0
10	12.0
11	18.0
12	12.0
13	3.0
14	163.0
15	478.0
16	602.0
17	753.0
18	927.0
19	1186.0
20	1750.0
21	2603.0
22	3887.0
23	6058.0
24	9244.0
25	14152.0
26	20970.0
27	30200.0
28	41580.0
29	55758.0
30	75684.0
31	100644.0
32	137910.0
33	204648.0
34	444960.0
35	867058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18562180207936	17.33432727885125	11.57423138457271	25.905819534496676
2	17.052020689276237	19.305734101516848	37.15654618382448	26.48569902538243
3	18.9179761371092	23.307633769839974	28.36194722930601	29.412442863744815
4	12.888131152212717	15.12999054038543	35.749130229999246	36.232748077402604
5	15.150281307451333	35.93398427527957	33.266708752293106	15.649025664975985
6	34.687348022755955	35.05716447011364	16.65237699212328	13.603110515007128
7	30.818323852532902	30.122180125643283	20.621359357792446	18.438136664031372
8	28.372027671725043	32.37228691480451	19.347202178433452	19.908483235037
9	27.38351265119818	14.508890693034235	18.63955897056852	39.468037685199064
10	16.307280228410963	25.83817393924312	30.927353063842567	26.92719276850335
11	37.690903756652474	21.13068159610853	22.118688469113774	19.05972617812522
12	25.39790127793472	23.334405287869266	27.994340080050268	23.273353354145744
13	29.21313511413932	19.085846328170035	25.328217041605743	26.3728015160849
14	23.874615372055636	19.718283378085992	24.62786519678058	31.779236053077792
15	25.31223881128796	26.97375160146258	22.084521595064153	25.629487992185307
16	26.30908898391044	25.55028000130624	23.242186533614507	24.89844448116881
17	24.48849924314663	25.75256065837089	24.786787057238303	24.97215304124418
18	25.473932007900352	24.409205333924408	25.820858747580615	24.296003910594624
19	25.823286595614253	24.77259087335495	25.141195277707546	24.26292725332325
20	25.958739719439595	24.083884128442502	24.616793548683955	25.340582603433955
21	27.225661661132722	23.940869687402746	24.249355445354976	24.58411320610955
22	26.13120173841883	23.958326633342356	24.70033104964656	25.210140578592256
23	24.53062549292611	23.804830453209995	25.505048256043512	26.159495797820387
24	25.092058113632515	24.799283129293034	24.911504468566644	25.1971542885078
25	25.127130993539165	24.27411505596139	25.01634134888248	25.582412601616966
26	24.95180365183829	25.182542431589887	25.405710327081994	24.459943589489832
27	25.66040181721206	24.478729099439022	24.510394741920067	25.350474341428853
28	24.789948811928117	24.390626437901933	25.504929313359465	25.314495436810486
29	24.79728579684445	24.67739892492697	25.374949531733655	25.15036574649493
30	24.57025954621466	24.877450252817603	25.63164821246574	24.920641988501995
31	25.50051331527453	24.693655917968506	24.279243509344813	25.526587257412146
32	25.010612727951003	24.55394382824104	24.3517330164193	26.083710427388656
33	24.53861183526633	24.29612360220451	25.169496850522656	25.995767712006508
34	25.518717572420563	24.324680041285635	25.24732291560492	24.909279470688883
35	26.184533717572407	24.239125486648945	25.140774402710786	24.435566393067862
36	24.778054759192752	24.976422493090034	24.708188148999948	25.537334598717266
37	25.986888328138143	24.65083370441839	24.501706991242393	24.86057097620108
38	24.867984814728505	24.468958310759763	25.23589071472994	25.427166159781788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	162.5
2	221.0
3	221.0
4	502.0
5	783.0
6	783.0
7	934.0
8	1085.0
9	1101.0
10	1117.0
11	1117.0
12	1470.0
13	1823.0
14	2687.5
15	3552.0
16	3552.0
17	5326.0
18	7100.0
19	7100.0
20	8961.5
21	10823.0
22	12152.0
23	13481.0
24	13481.0
25	16277.5
26	19074.0
27	19074.0
28	24315.5
29	29557.0
30	36125.5
31	42694.0
32	42694.0
33	55318.0
34	67942.0
35	67942.0
36	76395.5
37	84849.0
38	96295.0
39	107741.0
40	107741.0
41	115131.5
42	122522.0
43	139451.5
44	156381.0
45	156381.0
46	160022.0
47	163663.0
48	163663.0
49	175153.0
50	186643.0
51	189766.0
52	192889.0
53	192889.0
54	184529.5
55	176170.0
56	176170.0
57	170465.0
58	164760.0
59	147682.0
60	130604.0
61	130604.0
62	123953.5
63	117303.0
64	97784.5
65	78266.0
66	78266.0
67	65750.5
68	53235.0
69	53235.0
70	43083.0
71	32931.0
72	25937.5
73	18944.0
74	18944.0
75	14368.5
76	9793.0
77	9793.0
78	9431.5
79	9070.0
80	7186.5
81	5303.0
82	5303.0
83	5312.0
84	5321.0
85	5321.0
86	3604.0
87	1887.0
88	1585.0
89	1283.0
90	1283.0
91	945.0
92	607.0
93	533.0
94	459.0
95	459.0
96	417.0
97	375.0
98	375.0
99	649.5
100	924.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11819249099208967
2	0.054519935149971874
3	0.011527354709567556
4	0.0027210493949622987
5	1.4842087608885266E-4
6	2.968417521777053E-4
7	4.947362536295089E-5
8	4.947362536295088E-4
9	3.957890029036071E-4
10	4.947362536295088E-4
11	0.0018799977637921337
12	0.002424207642784593
13	0.014050509603078052
14	0.00841051631170165
15	0.023252603920586916
16	0.010735776703760342
17	0.020383133649535763
18	0.005095783412383941
19	0.007025254801539026
20	0.005442098789924597
21	0.005738940542102302
22	0.006530518547909516
23	0.009152620692145914
24	0.013506299724085593
25	0.016425243620499692
26	0.018750504012558386
27	0.007965253683435094
28	0.005194730663109843
29	0.010241040450130834
30	0.002918943896414102
31	0.005145257037746892
32	0.005986308668917057
33	0.007421043804442633
34	0.01172524921101936
35	0.015584191989329529
36	0.014099983228441
37	0.008855778939968209
38	0.005442098789924597
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2021279.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.99196294293503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.72513746742189	44.94225731114735
2	11.169004478618204	12.284109607952955
3	3.1189324838769634	5.145486587246349
4	1.2887136704710176	2.83475577642384
5	0.6710167765215211	1.8450264854279605
6	0.4234080205578913	1.3970422905756619
7	0.3048292307852798	1.1734210434287249
8	0.20856392891817083	0.917547188024079
9	0.1512608522792581	0.7486318064931958
>10	0.7842542964000968	7.666994667105074
>50	0.06407523519487099	2.4787166713779047
>100	0.07264464962104843	8.777453666040422
>500	0.012467310885826227	4.761803270998957
>1k	0.005691598447877191	5.026753627757515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3899	0.1928976652901455	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2788	0.13793246751190705	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2708	0.133974577482871	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2467	0.12205143377039983	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2442	0.12081459313632605	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2436	0.12051775138414834	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2416	0.11952827887688933	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2358	0.11665880860583819	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2331	0.1153230207210385	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2300	0.11378933833478705	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2157	0.10671460990788505	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2110	0.10438934951582637	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2055	0.10166830012086406	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2027	0.10028303861070144	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4842087608885266E-4
2	0.0	0.0	0.0	0.0	1.4842087608885266E-4
3	0.0	0.0	0.0	0.0	1.4842087608885266E-4
4	0.0	0.0	0.0	0.0	1.4842087608885266E-4
5	0.0	0.0	0.0	0.0	1.4842087608885266E-4
6	0.0	0.0	0.0	0.0	1.4842087608885266E-4
7	0.0	0.0	0.0	0.0	1.4842087608885266E-4
8	0.0	0.0	0.0	4.947362536295088E-5	1.4842087608885266E-4
9	0.0	0.0	0.0	4.947362536295088E-5	1.4842087608885266E-4
10	0.0	0.0	0.0	4.947362536295088E-5	1.9789450145180353E-4
11	0.0	0.0	0.0	4.947362536295088E-5	1.9789450145180353E-4
12	0.0	0.0	0.0	9.894725072590176E-5	3.4631537754065616E-4
13	0.0	0.0	0.0	9.894725072590176E-5	3.9578900290360706E-4
14	0.0	0.0	0.0	9.894725072590176E-5	3.9578900290360706E-4
15	0.0	0.0	0.0	1.4842087608885266E-4	6.431571297183615E-4
16	0.0	0.0	0.0	2.473681268147544E-4	6.431571297183615E-4
17	0.0	0.0	0.0	2.473681268147544E-4	6.431571297183615E-4
18	0.0	0.0	4.947362536295088E-5	2.473681268147544E-4	6.431571297183615E-4
19	0.0	0.0	4.947362536295088E-5	2.473681268147544E-4	6.431571297183615E-4
20	0.0	0.0	4.947362536295088E-5	3.9578900290360706E-4	6.431571297183615E-4
21	0.0	0.0	9.894725072590176E-5	4.947362536295088E-4	7.421043804442633E-4
22	0.0	0.0	9.894725072590176E-5	6.926307550813123E-4	7.421043804442633E-4
23	0.0	0.0	9.894725072590176E-5	0.0012368406340737721	7.915780058072141E-4
24	0.0	0.0	9.894725072590176E-5	0.0019294713891550846	8.410516311701651E-4
25	0.0	0.0	9.894725072590176E-5	0.002127365890606888	8.410516311701651E-4
26	0.0	0.0	9.894725072590176E-5	0.0024736812681475442	8.905252565331159E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTT	65	9.452549E-6	19.689188	4
CTAGCGG	640	0.0	17.749382	29
TCTAGCG	645	0.0	17.611355	28
ACGAATT	65	2.1019549E-4	17.23017	21
TAGCGGC	700	0.0	16.456572	30
CGCAAGA	695	0.0	16.120928	2
CAAGACG	725	0.0	16.107792	4
AAGACGG	730	0.0	15.997465	5
GTATCAA	3110	0.0	15.860018	1
GCGCAAG	715	0.0	15.678531	1
GACGGAC	710	0.0	15.323031	7
ACGATTA	95	1.4339335E-5	15.157369	32
AGACGGA	765	0.0	15.0564375	6
TCTAGAT	465	0.0	14.8010435	2
GTACGAA	65	0.0041639935	14.768717	19
TACGTTG	65	0.0041674185	14.766891	5
GTATTAG	430	0.0	14.524779	1
CATCGTT	420	0.0	14.475328	28
ATCGTTT	415	0.0	14.264564	29
GTTATTC	340	0.0	14.116806	3
>>END_MODULE