FastQCFastQC Report
Thu 2 Feb 2017
SRR4062845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2021279
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43870.21704079446726554No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28580.14139562128731362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28310.14005983340251393No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27820.13763562575972935No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26390.13056089733282739No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25860.12793879518859097No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24940.12338722165519951No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24850.12294195902693296No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA23610.11680722948192704No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23290.11522407347031262No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG23220.11487775809277195No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23110.11433354821377949No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22400.11082092081300997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG350.00206729322.85673714
TCTAGCG5500.018.90875428
CTATACG652.1016817E-417.2304632
CTAGCGG6100.017.04887829
GTTAGAC1304.807589E-915.9997163
TCTATAC1407.4396667E-1015.9997163
TAGCGGC6550.015.8775830
GTATCAA30550.015.6076961
GTTATTC3900.015.5894663
AAGACGG6000.015.4675395
CAAGACG6100.014.9509244
TACCGTC5100.014.745937
ATACCGT5450.014.67972856
ACCGTCC1757.4578566E-1114.6293978
ACCGTCG4950.014.5462768
TCGTTAG1103.5460853E-614.54519614
TTCGGAA6400.014.24974731
AGCGGCG7650.014.2219731
GTTTTCG6300.014.22196928
TTAGGAC3050.014.1636833
Produced by FastQC (version 0.11.5)