##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2021279
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204347346407893	32.0	32.0	32.0	32.0	32.0
2	31.238102706256782	32.0	32.0	32.0	32.0	32.0
3	31.330884059053698	32.0	32.0	32.0	32.0	32.0
4	31.427759849085653	32.0	32.0	32.0	32.0	32.0
5	31.349742910305803	32.0	32.0	32.0	32.0	32.0
6	34.86005890329836	36.0	36.0	36.0	32.0	36.0
7	34.882380413589615	36.0	36.0	36.0	32.0	36.0
8	34.81312525386154	36.0	36.0	36.0	32.0	36.0
9	34.932531332883784	36.0	36.0	36.0	32.0	36.0
10	34.774062363483715	36.0	36.0	36.0	32.0	36.0
11	34.95917238540548	36.0	36.0	36.0	36.0	36.0
12	34.84994698901043	36.0	36.0	36.0	32.0	36.0
13	34.911497621060725	36.0	36.0	36.0	32.0	36.0
14	34.85062923030418	36.0	36.0	36.0	32.0	36.0
15	34.81201308676338	36.0	36.0	36.0	32.0	36.0
16	34.82826764637638	36.0	36.0	36.0	32.0	36.0
17	34.793166109181364	36.0	36.0	36.0	32.0	36.0
18	34.79688553633615	36.0	36.0	36.0	32.0	36.0
19	34.78923691385504	36.0	36.0	36.0	32.0	36.0
20	34.77951336752621	36.0	36.0	36.0	32.0	36.0
21	34.76984325271276	36.0	36.0	36.0	32.0	36.0
22	34.756353279285044	36.0	36.0	36.0	32.0	36.0
23	34.69992762008609	36.0	36.0	36.0	32.0	36.0
24	34.66915700405535	36.0	36.0	36.0	32.0	36.0
25	34.65356291734095	36.0	36.0	36.0	32.0	36.0
26	34.587625458929715	36.0	36.0	36.0	32.0	36.0
27	34.57000493252045	36.0	36.0	36.0	32.0	36.0
28	34.53593838356803	36.0	36.0	36.0	32.0	36.0
29	34.4993828165236	36.0	36.0	36.0	32.0	36.0
30	34.485899274667176	36.0	36.0	36.0	32.0	36.0
31	34.485058223036006	36.0	36.0	36.0	32.0	36.0
32	34.45868927545381	36.0	36.0	36.0	32.0	36.0
33	34.4219115718315	36.0	36.0	36.0	32.0	36.0
34	34.41885707020159	36.0	36.0	36.0	32.0	36.0
35	34.38552965721209	36.0	36.0	36.0	32.0	36.0
36	34.36040051868149	36.0	36.0	36.0	32.0	36.0
37	34.36052816063493	36.0	36.0	36.0	32.0	36.0
38	33.91941043270128	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	15.0
21	42.0
22	143.0
23	500.0
24	1378.0
25	3582.0
26	7513.0
27	14528.0
28	25039.0
29	39398.0
30	59187.0
31	85803.0
32	122063.0
33	195405.0
34	469032.0
35	997648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33928585565117	18.732732209723004	12.287492380401998	26.64048955422383
2	15.939667873493898	20.483228927440955	36.355909479052364	27.22119372001278
3	17.86215559554124	24.11982709957408	28.67768378338666	29.340333521498025
4	12.335938787091106	16.09545073822289	35.72990882478517	35.83870164990083
5	14.632467858222443	36.51618603864187	32.862954594590846	15.988391508544836
6	34.589289142106274	35.60815049503383	16.271602690577637	13.530957672282263
7	30.592533114065358	30.44579376046062	20.557065331665076	18.404607793808943
8	28.24508604401373	32.74471572550023	19.115905984119593	19.89429224636645
9	27.45741999163861	14.290110553410235	18.45982203597377	39.79264741897739
10	16.09308055371402	26.16595588890098	30.85095435520418	26.89000920218082
11	37.78847010162381	21.068088938145042	22.044498758151178	19.098942202079975
12	25.17388897326966	23.47776957057995	27.887838334584202	23.46050312156619
13	29.20312476809135	19.1404414056509	25.258129789686684	26.39830403657107
14	23.75972033535219	19.725094717302618	24.56188609384756	31.953298853497635
15	25.326785663928632	27.072363587609626	21.918250770922768	25.682599977538974
16	26.260600342654328	25.654301063831365	23.074202027528116	25.01089656598619
17	24.42404042193087	25.87163870005081	24.656368566635283	25.047952311383042
18	25.51785280508035	24.45189407301021	25.697738906900035	24.332514215009407
19	25.76131251549143	24.891170392607847	25.064080713251364	24.28343637864936
20	26.02485851779987	24.021671426853988	24.593883377801877	25.359586677544264
21	27.14920955949652	24.103265359836698	24.147593750092764	24.599931330574023
22	26.0528605897553	24.076389256505408	24.696788518556815	25.173961635182472
23	24.353750448973372	23.827697130231467	25.496740181212086	26.321812239583075
24	24.98808922469387	24.850700967060956	24.84842518029426	25.31278462795092
25	25.07137814361911	24.286428826924762	25.028682362684577	25.61351066677155
26	24.938974252923153	25.168383567228258	25.367960270680236	24.524681909168358
27	25.80278417911836	24.416087247770964	24.455567220342772	25.3255613527679
28	24.710542186407718	24.462382481587152	25.469022336847114	25.358052995158015
29	24.77248316536213	24.64528647455398	25.394564530675872	25.18766582940801
30	24.678631698048612	24.736070577095	25.632829510423843	24.952468214432542
31	25.496925461551818	24.51462662997043	24.39455414121455	25.593893767263204
32	24.983982913788743	24.56662341022689	24.30708477157285	26.142308904411514
33	24.578447606688634	24.246133265125692	25.192563718318944	25.982855409866723
34	25.565298011803417	24.228421707245758	25.23986050416593	24.9664197767849
35	26.17629728503586	24.230598546761726	25.037859691809	24.555244476393412
36	24.860942007511085	24.901312485807253	24.71821059833897	25.51953490834269
37	26.010610113695336	24.6090717807883	24.527687667066246	24.85263043845011
38	24.90609891969231	24.29405979193341	25.24781375725037	25.552027531123905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	83.5
2	97.0
3	97.0
4	393.5
5	690.0
6	690.0
7	847.0
8	1004.0
9	1044.5
10	1085.0
11	1085.0
12	1437.0
13	1789.0
14	2675.5
15	3562.0
16	3562.0
17	5473.0
18	7384.0
19	7384.0
20	9234.5
21	11085.0
22	12398.5
23	13712.0
24	13712.0
25	16273.0
26	18834.0
27	18834.0
28	24015.5
29	29197.0
30	36225.0
31	43253.0
32	43253.0
33	56243.0
34	69233.0
35	69233.0
36	76954.0
37	84675.0
38	96150.5
39	107626.0
40	107626.0
41	115723.0
42	123820.0
43	140742.5
44	157665.0
45	157665.0
46	160660.5
47	163656.0
48	163656.0
49	175201.0
50	186746.0
51	189467.0
52	192188.0
53	192188.0
54	184170.5
55	176153.0
56	176153.0
57	170652.5
58	165152.0
59	148006.0
60	130860.0
61	130860.0
62	124307.0
63	117754.0
64	98119.5
65	78485.0
66	78485.0
67	65764.5
68	53044.0
69	53044.0
70	42986.0
71	32928.0
72	25847.5
73	18767.0
74	18767.0
75	14069.5
76	9372.0
77	9372.0
78	9276.5
79	9181.0
80	7145.5
81	5110.0
82	5110.0
83	4874.5
84	4639.0
85	4639.0
86	2976.5
87	1314.0
88	1079.0
89	844.0
90	844.0
91	516.0
92	188.0
93	126.0
94	64.0
95	64.0
96	44.5
97	25.0
98	25.0
99	26.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008657884438516405
2	4.947362536295089E-5
3	0.0
4	2.968417521777053E-4
5	0.0
6	1.4842087608885266E-4
7	3.957890029036071E-4
8	0.0022757867666957404
9	0.004155784530487874
10	9.399988818960669E-4
11	0.004897888910932137
12	8.905252565331159E-4
13	4.4526262826655795E-4
14	4.947362536295089E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.947362536295089E-5
22	0.0
23	4.947362536295089E-5
24	0.0
25	9.894725072590178E-5
26	4.947362536295089E-5
27	4.947362536295089E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.4842087608885266E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2021279.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.04294795377245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4122255098718	42.653014925671435
2	11.93350536624705	12.659766080958132
3	3.35430589473569	5.33766818986492
4	1.3683887897556468	2.9033350142213776
5	0.753700366757114	1.9989244663318404
6	0.4735793947980604	1.5072028314151542
7	0.3014600333784323	1.1193230202444282
8	0.21879758747998376	0.9284535236089402
9	0.16262621874018876	0.776355665089918
>10	0.85682026925237	8.0404538088249
>50	0.06872605421644133	2.5830376819908825
>100	0.0761678313394025	8.999489879116851
>500	0.012474566171055636	4.521068712783526
>1k	0.007222117256926948	5.97190619987767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4387	0.21704079446726554	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2858	0.14139562128731362	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2831	0.14005983340251393	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2782	0.13763562575972935	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2639	0.13056089733282739	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2586	0.12793879518859097	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2494	0.12338722165519951	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2485	0.12294195902693296	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2361	0.11680722948192704	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2329	0.11522407347031262	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2322	0.11487775809277195	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2311	0.11433354821377949	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2240	0.11082092081300997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4842087608885266E-4
2	0.0	0.0	0.0	0.0	1.4842087608885266E-4
3	0.0	0.0	0.0	0.0	1.4842087608885266E-4
4	0.0	0.0	0.0	0.0	1.4842087608885266E-4
5	0.0	0.0	0.0	0.0	1.4842087608885266E-4
6	0.0	0.0	0.0	0.0	1.9789450145180353E-4
7	0.0	0.0	0.0	4.947362536295088E-5	1.9789450145180353E-4
8	0.0	0.0	0.0	4.947362536295088E-5	1.9789450145180353E-4
9	0.0	0.0	0.0	9.894725072590176E-5	1.9789450145180353E-4
10	0.0	0.0	4.947362536295088E-5	9.894725072590176E-5	1.9789450145180353E-4
11	0.0	0.0	4.947362536295088E-5	9.894725072590176E-5	1.9789450145180353E-4
12	0.0	0.0	4.947362536295088E-5	1.4842087608885266E-4	2.968417521777053E-4
13	0.0	0.0	4.947362536295088E-5	1.4842087608885266E-4	3.4631537754065616E-4
14	0.0	0.0	4.947362536295088E-5	1.4842087608885266E-4	3.9578900290360706E-4
15	0.0	0.0	4.947362536295088E-5	1.4842087608885266E-4	3.9578900290360706E-4
16	0.0	0.0	4.947362536295088E-5	2.968417521777053E-4	3.9578900290360706E-4
17	0.0	0.0	4.947362536295088E-5	2.968417521777053E-4	3.9578900290360706E-4
18	0.0	0.0	4.947362536295088E-5	2.968417521777053E-4	3.9578900290360706E-4
19	0.0	0.0	4.947362536295088E-5	2.968417521777053E-4	3.9578900290360706E-4
20	0.0	0.0	4.947362536295088E-5	4.4526262826655795E-4	3.9578900290360706E-4
21	0.0	0.0	4.947362536295088E-5	5.442098789924597E-4	3.9578900290360706E-4
22	0.0	0.0	4.947362536295088E-5	7.915780058072141E-4	4.4526262826655795E-4
23	0.0	0.0	4.947362536295088E-5	0.0013357878847996738	4.4526262826655795E-4
24	0.0	0.0	4.947362536295088E-5	0.0019789450145180354	4.4526262826655795E-4
25	0.0	0.0	4.947362536295088E-5	0.002127365890606888	4.4526262826655795E-4
26	0.0	0.0	4.947362536295088E-5	0.0023747340174216426	4.947362536295088E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	35	0.002067293	22.856737	14
TCTAGCG	550	0.0	18.908754	28
CTATACG	65	2.1016817E-4	17.230463	2
CTAGCGG	610	0.0	17.048878	29
GTTAGAC	130	4.807589E-9	15.999716	3
TCTATAC	140	7.4396667E-10	15.999716	3
TAGCGGC	655	0.0	15.87758	30
GTATCAA	3055	0.0	15.607696	1
GTTATTC	390	0.0	15.589466	3
AAGACGG	600	0.0	15.467539	5
CAAGACG	610	0.0	14.950924	4
TACCGTC	510	0.0	14.74593	7
ATACCGT	545	0.0	14.6797285	6
ACCGTCC	175	7.4578566E-11	14.629397	8
ACCGTCG	495	0.0	14.546276	8
TCGTTAG	110	3.5460853E-6	14.545196	14
TTCGGAA	640	0.0	14.249747	31
AGCGGCG	765	0.0	14.22197	31
GTTTTCG	630	0.0	14.221969	28
TTAGGAC	305	0.0	14.163683	3
>>END_MODULE