FastQCFastQC Report
Thu 2 Feb 2017
SRR4062844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018343
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27340.2684753565350771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17580.17263338580419368No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14860.1459233283873901No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12670.1244178042172431No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12530.12304302185020174No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12180.11960606593259834No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11960.11744569364153334No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11880.11666010371750972No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11410.11204476291387087No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11390.11184836543286496No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11180.10978619188230293No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11110.10909880069878224No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11090.10890240321777633No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10830.10634923596469952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10300.10114470271804293No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10250.10065370901552817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG401.5950702E-423.9962445
CTAGGTC600.00244420115.9974963
GGTCTAC756.19523E-414.9486121
GTATAGA1103.5038847E-614.5603361
ATCGTTT2550.014.4312429
GCGACGA1901.8189894E-1114.31565832
GTCGTAA901.5340329E-414.22209130
CGTAAAC901.5340329E-414.22209132
TCGTTTA2600.014.15371530
GACTGCG800.001018632413.9984967
CATCGTT2750.013.96350828
TTAACAC1155.621365E-613.9108663
CTAGCGG1851.8189894E-1013.83771129
GTCCTAA1053.6467012E-513.7283181
CCCCTAT700.00675718413.7283171
GGGTATA700.00675718413.7283171
TACTAAG700.00678141313.7208982
CGTGCAA700.006803503313.7141611
AACCCCG700.006807928413.7128127
TACACCG700.006810141713.7121395
Produced by FastQC (version 0.11.5)