##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062844_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018343
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223102628485687	32.0	32.0	32.0	32.0	32.0
2	30.855812825344703	32.0	32.0	32.0	32.0	32.0
3	30.91508263915007	32.0	32.0	32.0	32.0	32.0
4	30.978444394472195	32.0	32.0	32.0	32.0	32.0
5	30.894505093077676	32.0	32.0	32.0	32.0	32.0
6	34.55212732841488	36.0	36.0	36.0	32.0	36.0
7	34.458043115139006	36.0	36.0	36.0	32.0	36.0
8	34.44852863917168	36.0	36.0	36.0	32.0	36.0
9	34.57346886068839	36.0	36.0	36.0	32.0	36.0
10	34.309191500309815	36.0	36.0	36.0	32.0	36.0
11	34.57253891861583	36.0	36.0	36.0	32.0	36.0
12	34.392790052074794	36.0	36.0	36.0	32.0	36.0
13	34.48425628692886	36.0	36.0	36.0	32.0	36.0
14	34.38000654003612	36.0	36.0	36.0	32.0	36.0
15	34.327251230675714	36.0	36.0	36.0	32.0	36.0
16	34.34311523720397	36.0	36.0	36.0	32.0	36.0
17	34.26737062070442	36.0	36.0	36.0	32.0	36.0
18	34.28783622021264	36.0	36.0	36.0	32.0	36.0
19	34.28757304758809	36.0	36.0	36.0	32.0	36.0
20	34.26568847627961	36.0	36.0	36.0	32.0	36.0
21	34.24305661255589	36.0	36.0	36.0	32.0	36.0
22	34.21822804300712	36.0	36.0	36.0	32.0	36.0
23	34.15655236005943	36.0	36.0	36.0	32.0	36.0
24	34.14332204375147	36.0	36.0	36.0	32.0	36.0
25	34.13120824712302	36.0	36.0	36.0	32.0	36.0
26	34.07621106051694	36.0	36.0	36.0	32.0	36.0
27	34.083531776621435	36.0	36.0	36.0	32.0	36.0
28	34.05654578074382	36.0	36.0	36.0	32.0	36.0
29	34.02749466535342	36.0	36.0	36.0	32.0	36.0
30	33.99386356070597	36.0	36.0	36.0	32.0	36.0
31	34.01274521452988	36.0	36.0	36.0	32.0	36.0
32	33.96376073680479	36.0	36.0	36.0	32.0	36.0
33	33.91875134409526	36.0	36.0	36.0	32.0	36.0
34	33.924588277230754	36.0	36.0	36.0	32.0	36.0
35	33.875339644893714	36.0	36.0	36.0	32.0	36.0
36	33.83479240295264	36.0	36.0	36.0	32.0	36.0
37	33.822084503944154	36.0	36.0	36.0	32.0	36.0
38	33.22652583657962	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	1.0
9	2.0
10	8.0
11	11.0
12	8.0
13	3.0
14	155.0
15	299.0
16	402.0
17	457.0
18	537.0
19	725.0
20	1024.0
21	1296.0
22	2012.0
23	2997.0
24	4402.0
25	6676.0
26	9754.0
27	13848.0
28	19726.0
29	26945.0
30	36017.0
31	48483.0
32	66276.0
33	98957.0
34	219870.0
35	457448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35598692774472	17.350724991053134	11.44510557301232	25.848182508189822
2	16.5437365578456	20.116291522685984	37.391025823674305	25.948946095794113
3	18.52012625930298	24.593752639404766	28.727134161312563	28.15898693997969
4	12.493003195452912	15.680600045957958	36.34881697537714	35.477579783211986
5	14.549350364956338	36.516844554034456	33.74105530465728	15.19274977635193
6	33.63028778264191	35.79646443518753	17.145191478664255	13.428056303506303
7	29.8294678997822	30.75047479137657	21.38122556076446	18.038831748076774
8	28.08727929768054	32.431113380599804	19.99617023783805	19.48543708388161
9	27.783255101439597	14.245635053125675	18.75748767602176	39.213622169412965
10	15.726197268296707	26.60518746704192	31.820641259934067	25.847974004727302
11	37.04997044156956	21.348551833310093	22.434063095955896	19.167414629164448
12	24.993764202397294	23.865035735539333	28.666698091158523	22.474501970904846
13	29.527734210420352	19.463101749230766	25.32107532858845	25.688088711760436
14	23.880246695343036	19.903855596802387	25.263881523383024	30.95201618447155
15	25.176455103888983	26.688576631509882	22.947315898344574	25.187652366256557
16	25.766389164143543	25.742033628316847	23.792314060706175	24.699263146833434
17	24.00524095012847	25.743417382353634	25.50170114798023	24.749640519537667
18	25.09447154854777	24.536873364915508	26.327610046587637	24.04104503994909
19	25.554343268089134	25.218972833521725	25.66462760133048	23.56205629705866
20	25.759507096196376	24.0425143279138	25.334579865107454	24.86339871078237
21	27.021206389610697	24.274706907151092	24.444405162440905	24.259681540797306
22	25.71768079506619	24.353026672428015	24.911517460816277	25.017775071689513
23	24.189276381110073	24.17925926362402	25.64873111601492	25.982733239250994
24	24.584250373936502	25.294410545451328	25.226547085554117	24.894791995058046
25	24.790706627852614	24.44597657799935	25.37910484114918	25.384211952998857
26	24.69442335514071	25.238150894213764	25.705171206405296	24.36225454424023
27	25.524444963845433	24.85075132553643	24.737716139283705	24.887087571334433
28	24.44266804414071	24.55491515669913	25.893336763924584	25.109080035235575
29	24.3031030378265	25.242676492887643	25.731859662079614	24.722360807206243
30	24.508943213979183	24.991996574730386	26.008087878591475	24.490972332698952
31	24.941371955171643	25.01610552655251	24.69792317271017	25.344599345565676
32	24.428864780674058	25.116593782254178	24.69824085540407	25.756300581667695
33	24.288274663275278	24.498141446480044	25.422065965146594	25.791517925098084
34	24.96439914597234	24.78025679755341	25.60148256687559	24.653861489598658
35	25.907683785832987	24.580532628295533	25.336870307877856	24.174913277993628
36	24.565140743054183	25.448363440296283	24.920963697808787	25.065532118840743
37	25.491085721244406	25.330614954618397	24.84782775221114	24.33047157192606
38	24.444335328518047	25.073628699234497	25.525663247519116	24.956372724728343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	407.0
1	332.0
2	257.0
3	257.0
4	578.0
5	899.0
6	899.0
7	1015.0
8	1131.0
9	1084.5
10	1038.0
11	1038.0
12	1334.0
13	1630.0
14	2075.5
15	2521.0
16	2521.0
17	3603.5
18	4686.0
19	4686.0
20	5745.5
21	6805.0
22	7316.0
23	7827.0
24	7827.0
25	9093.0
26	10359.0
27	10359.0
28	13151.5
29	15944.0
30	19406.5
31	22869.0
32	22869.0
33	28923.5
34	34978.0
35	34978.0
36	39236.0
37	43494.0
38	49654.0
39	55814.0
40	55814.0
41	60219.0
42	64624.0
43	72799.5
44	80975.0
45	80975.0
46	84944.5
47	88914.0
48	88914.0
49	93059.0
50	97204.0
51	96371.5
52	95539.0
53	95539.0
54	90627.5
55	85716.0
56	85716.0
57	82744.5
58	79773.0
59	70628.5
60	61484.0
61	61484.0
62	58315.5
63	55147.0
64	45278.0
65	35409.0
66	35409.0
67	29545.0
68	23681.0
69	23681.0
70	19062.5
71	14444.0
72	11435.0
73	8426.0
74	8426.0
75	6393.0
76	4360.0
77	4360.0
78	4354.5
79	4349.0
80	3430.5
81	2512.0
82	2512.0
83	2541.0
84	2570.0
85	2570.0
86	1698.5
87	827.0
88	707.5
89	588.0
90	588.0
91	431.5
92	275.0
93	240.5
94	206.0
95	206.0
96	182.0
97	158.0
98	158.0
99	330.5
100	503.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12048985459712494
2	0.05459849971964259
3	0.011882047600857471
4	0.0020621735505620404
5	1.9639748100590862E-4
6	4.909937025147715E-4
7	9.819874050295431E-5
8	2.9459622150886295E-4
9	2.9459622150886295E-4
10	3.9279496201181723E-4
11	0.0020621735505620404
12	0.0028477634745856747
13	0.012176643822366334
14	0.008150495461745207
15	0.02327310149920017
16	0.009230681607277705
17	0.02032713928411154
18	0.004615340803638852
19	0.006481116873194984
20	0.00500813576565067
21	0.0063829181326920306
22	0.006186520651686122
23	0.008445091683254071
24	0.011980246341360426
25	0.015220804777957918
26	0.0182649657335495
27	0.0074631042782245275
28	0.005302731987159533
29	0.011292855157839746
30	0.0028477634745856747
31	0.005793725689674304
32	0.006284719392189076
33	0.0076595017592304354
34	0.010703662714822018
35	0.014435214853934284
36	0.013747823670413604
37	0.008739687904762934
38	0.005695526949171349
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1018343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.680089188409646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.70491592907328	50.79221764084498
2	10.702341940375048	12.9883812693363
3	2.650617640316338	4.8251914445630195
4	1.020385593100721	2.4766835518368016
5	0.5232763654859635	1.5876228263937555
6	0.28706529806993786	1.0451488733868737
7	0.2006230684088537	0.8521677979011157
8	0.12964942249483621	0.6293710816169973
9	0.0917990306552409	0.5013336030812612
>10	0.5306814510973739	6.097289445017051
>50	0.06860068836575438	2.9329292367484223
>100	0.07996649446705688	10.120059728094306
>500	0.00731400829077268	2.995946309976611
>1k	0.0027630697987363452	2.155657191202515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2734	0.2684753565350771	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1758	0.17263338580419368	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1486	0.1459233283873901	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1267	0.1244178042172431	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1253	0.12304302185020174	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1218	0.11960606593259834	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1196	0.11744569364153334	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1188	0.11666010371750972	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1141	0.11204476291387087	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1139	0.11184836543286496	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1118	0.10978619188230293	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1111	0.10909880069878224	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1109	0.10890240321777633	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1083	0.10634923596469952	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1030	0.10114470271804293	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1025	0.10065370901552817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9639748100590862E-4
2	0.0	0.0	0.0	0.0	1.9639748100590862E-4
3	0.0	0.0	0.0	0.0	1.9639748100590862E-4
4	0.0	0.0	0.0	0.0	1.9639748100590862E-4
5	0.0	0.0	0.0	0.0	1.9639748100590862E-4
6	0.0	0.0	0.0	0.0	3.9279496201181723E-4
7	0.0	0.0	0.0	9.819874050295431E-5	4.909937025147715E-4
8	0.0	0.0	0.0	9.819874050295431E-5	4.909937025147715E-4
9	0.0	0.0	0.0	9.819874050295431E-5	4.909937025147715E-4
10	0.0	0.0	0.0	9.819874050295431E-5	4.909937025147715E-4
11	0.0	0.0	0.0	9.819874050295431E-5	4.909937025147715E-4
12	0.0	0.0	0.0	1.9639748100590862E-4	5.891924430177259E-4
13	0.0	0.0	0.0	1.9639748100590862E-4	5.891924430177259E-4
14	0.0	0.0	0.0	1.9639748100590862E-4	5.891924430177259E-4
15	0.0	0.0	0.0	1.9639748100590862E-4	9.81987405029543E-4
16	0.0	0.0	0.0	1.9639748100590862E-4	9.81987405029543E-4
17	0.0	0.0	0.0	2.9459622150886295E-4	9.81987405029543E-4
18	0.0	0.0	0.0	3.9279496201181723E-4	9.81987405029543E-4
19	0.0	0.0	0.0	3.9279496201181723E-4	9.81987405029543E-4
20	0.0	0.0	0.0	4.909937025147715E-4	0.0010801861455324974
21	0.0	0.0	0.0	0.0011783848860354518	0.0011783848860354518
22	0.0	0.0	0.0	0.001276583626538406	0.0011783848860354518
23	0.0	0.0	0.0	0.0023567697720709036	0.0011783848860354518
24	0.0	0.0	0.0	0.0032405584365974923	0.001276583626538406
25	0.0	0.0	0.0	0.003829750879615218	0.001276583626538406
26	0.0	0.0	0.0	0.004615340803638852	0.001571179848047269
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCCG	40	1.5950702E-4	23.996244	5
CTAGGTC	60	0.002444201	15.997496	3
GGTCTAC	75	6.19523E-4	14.948612	1
GTATAGA	110	3.5038847E-6	14.560336	1
ATCGTTT	255	0.0	14.43124	29
GCGACGA	190	1.8189894E-11	14.315658	32
GTCGTAA	90	1.5340329E-4	14.222091	30
CGTAAAC	90	1.5340329E-4	14.222091	32
TCGTTTA	260	0.0	14.153715	30
GACTGCG	80	0.0010186324	13.998496	7
CATCGTT	275	0.0	13.963508	28
TTAACAC	115	5.621365E-6	13.910866	3
CTAGCGG	185	1.8189894E-10	13.837711	29
GTCCTAA	105	3.6467012E-5	13.728318	1
CCCCTAT	70	0.006757184	13.728317	1
GGGTATA	70	0.006757184	13.728317	1
TACTAAG	70	0.006781413	13.720898	2
CGTGCAA	70	0.0068035033	13.71416	11
AACCCCG	70	0.0068079284	13.712812	7
TACACCG	70	0.0068101417	13.712139	5
>>END_MODULE