FastQCFastQC Report
Thu 2 Feb 2017
SRR4062844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018343
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32390.318065720489069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23770.23341840617552237No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15010.14739630949493443No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14940.14670891831141372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14520.14258457121028967No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14070.1381656278876567No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12920.12687277272981698No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12560.12333761807171062No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12540.12314122059070472No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12380.12157004074265744No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12290.12068625207813084No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11900.11685650119851562No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11790.11577631505298312No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11710.1149907251289595No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10810.10615283848369361No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10620.10428706241413749No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10620.10428706241413749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.10193029264206659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG1400.021.7141867
CGCGGTC1400.021.71418610
TATAGGG555.8326616E-520.3635432
GTAGCGT507.1953924E-419.1999116
ATCGTTT2750.016.87264829
GGTCCTA1850.016.43235613
GTATAGG1203.103014E-816.0022851
TAGAACC2400.015.9999274
TAGGAGT1203.108653E-815.9999274
CGGCATC2800.015.99992625
ATAGACC1001.3140725E-615.9999263
ATAGAAC2700.015.9999263
CCAGTCG2850.015.71922620
CGTTTAT2850.015.71922631
AGAACCG2050.015.6096835
GAACCGC1950.015.5896726
ATACCGT1753.6379788E-1215.5427856
ACCGTCG1652.910383E-1115.51507958
TCGTTTA3000.015.46659530
TTTTCGG2800.015.428529
Produced by FastQC (version 0.11.5)