##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062844_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1018343 Sequences flagged as poor quality 0 Sequence length 38 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16947531430962 32.0 32.0 32.0 32.0 32.0 2 31.2618803291229 32.0 32.0 32.0 32.0 32.0 3 31.350059852132336 32.0 32.0 32.0 32.0 32.0 4 31.458242458582227 32.0 32.0 32.0 32.0 32.0 5 31.373894650427214 32.0 32.0 32.0 32.0 32.0 6 34.905867669341276 36.0 36.0 36.0 36.0 36.0 7 34.93667457821186 36.0 36.0 36.0 36.0 36.0 8 34.875429987734975 36.0 36.0 36.0 32.0 36.0 9 34.985178864095886 36.0 36.0 36.0 36.0 36.0 10 34.82068517189199 36.0 36.0 36.0 32.0 36.0 11 35.00484512585641 36.0 36.0 36.0 36.0 36.0 12 34.90298160835789 36.0 36.0 36.0 32.0 36.0 13 34.956724797047755 36.0 36.0 36.0 36.0 36.0 14 34.90213415322735 36.0 36.0 36.0 32.0 36.0 15 34.876925554552834 36.0 36.0 36.0 32.0 36.0 16 34.88587636974968 36.0 36.0 36.0 32.0 36.0 17 34.84641815184079 36.0 36.0 36.0 32.0 36.0 18 34.849649872390735 36.0 36.0 36.0 32.0 36.0 19 34.83972590767551 36.0 36.0 36.0 32.0 36.0 20 34.82944646351966 36.0 36.0 36.0 32.0 36.0 21 34.82172902450353 36.0 36.0 36.0 32.0 36.0 22 34.79391128529385 36.0 36.0 36.0 32.0 36.0 23 34.734366515015076 36.0 36.0 36.0 32.0 36.0 24 34.70862273320483 36.0 36.0 36.0 32.0 36.0 25 34.700809059423 36.0 36.0 36.0 32.0 36.0 26 34.63495698404172 36.0 36.0 36.0 32.0 36.0 27 34.60807704280385 36.0 36.0 36.0 32.0 36.0 28 34.58048221473511 36.0 36.0 36.0 32.0 36.0 29 34.53990060323486 36.0 36.0 36.0 32.0 36.0 30 34.52174758406549 36.0 36.0 36.0 32.0 36.0 31 34.516552870692884 36.0 36.0 36.0 32.0 36.0 32 34.48078594343949 36.0 36.0 36.0 32.0 36.0 33 34.44667268297617 36.0 36.0 36.0 32.0 36.0 34 34.42306275979704 36.0 36.0 36.0 32.0 36.0 35 34.386564251926906 36.0 36.0 36.0 32.0 36.0 36 34.36140278864783 36.0 36.0 36.0 32.0 36.0 37 34.340119193631224 36.0 36.0 36.0 32.0 36.0 38 33.8973999919477 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 21.0 22 80.0 23 246.0 24 653.0 25 1621.0 26 3567.0 27 6911.0 28 12114.0 29 19214.0 30 29096.0 31 41920.0 32 60122.0 33 96062.0 34 233374.0 35 513338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.610682869677 18.662269567207172 12.168167448566015 26.558880114549808 2 15.527479444548645 21.2067054027965 36.77415173472985 26.491663417925004 3 17.46081624757081 25.372590571153335 29.06044427074178 28.106148910534074 4 11.97780313489967 16.614064823016236 36.34800489423004 35.06012714785406 5 13.980260089184096 37.13513030481871 33.42027195159195 15.464337654405243 6 33.547996201665256 36.387418359861776 16.854275728857033 13.210309709615933 7 29.410892464871647 31.086958443030156 21.468138739925976 18.034010352172217 8 27.91229100422067 32.80710816421525 19.774491761937778 19.506109069626305 9 27.73310184116796 14.038697031682545 18.614464397768486 39.61373672938101 10 15.500317184735069 26.85189731463351 31.82943906419701 25.818346436434414 11 37.24206809048878 21.248718442254287 22.367552725544343 19.14166074171259 12 24.77534406653999 23.956163737866223 28.79543568668464 22.47305650890915 13 29.52547329430908 19.5183145920669 25.23025194900333 25.72596016462069 14 23.858562390078784 19.676965423241484 25.37003740390026 31.09443478277948 15 25.160481291666954 26.845768076178654 22.85163250496149 25.1421181271929 16 25.674846294421428 25.85189862354825 23.602951068549594 24.870304013480723 17 24.044158009629367 25.77108106011432 25.41835118422771 24.766409746028597 18 25.036456282411724 24.545855374858963 26.39758902452317 24.020099318206146 19 25.486206513915256 25.252198915296713 25.752226901937757 23.509367668850278 20 25.691343682825924 23.910902318766862 25.4677451507007 24.93000884770652 21 27.058493119207494 24.365979209342246 24.502966586863746 24.072561084586514 22 25.583816061975188 24.418491608426628 25.10814136297888 24.889550966619304 23 24.08024424038706 23.95346941741519 25.771131673967755 26.195154668229993 24 24.60438182419872 25.275668414276918 25.295111764896504 24.824837996627856 25 24.836523646747708 24.261373623621903 25.509479615414456 25.39262311421594 26 24.679528095669234 25.100899304948634 25.771400963526986 24.448171635855147 27 25.48655307624097 24.756146540879886 24.81094176092983 24.946358621949315 28 24.42674030262888 24.42114297442021 26.01598871892869 25.13612800402222 29 24.445889057026953 24.919796178694213 25.928984634843072 24.705330129435758 30 24.601141265762124 24.874722956803357 26.04417175745304 24.47996401998148 31 25.053444664518732 24.66271187605748 24.88984556284081 25.393997896582977 32 24.591615987933338 24.85154805404466 24.864804884012557 25.692031074009446 33 24.411028504148405 24.23702033597717 25.61249009420205 25.73946106567237 34 24.916162825295604 24.577475369300913 25.796416335164086 24.7099454702394 35 26.00204449777727 24.355153420802225 25.45095316607469 24.191848915345812 36 24.7897810462683 25.10823956171938 25.14584967933201 24.956129712680305 37 25.611606305537528 25.038911250924293 25.013772373355543 24.33571007018264 38 24.658292932734845 24.72929062211848 25.61042792065149 25.001988524495182 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 221.0 1 185.0 2 149.0 3 149.0 4 509.0 5 869.0 6 869.0 7 970.5 8 1072.0 9 1071.5 10 1071.0 11 1071.0 12 1398.5 13 1726.0 14 2166.5 15 2607.0 16 2607.0 17 3979.0 18 5351.0 19 5351.0 20 6217.0 21 7083.0 22 7554.5 23 8026.0 24 8026.0 25 9185.5 26 10345.0 27 10345.0 28 13176.5 29 16008.0 30 19491.5 31 22975.0 32 22975.0 33 29233.0 34 35491.0 35 35491.0 36 39389.0 37 43287.0 38 49440.0 39 55593.0 40 55593.0 41 60048.0 42 64503.0 43 72792.5 44 81082.0 45 81082.0 46 85214.0 47 89346.0 48 89346.0 49 93588.5 50 97831.0 51 96731.0 52 95631.0 53 95631.0 54 90315.0 55 84999.0 56 84999.0 57 82513.5 58 80028.0 59 70924.5 60 61821.0 61 61821.0 62 58508.0 63 55195.0 64 45349.0 65 35503.0 66 35503.0 67 29648.0 68 23793.0 69 23793.0 70 19127.0 71 14461.0 72 11361.0 73 8261.0 74 8261.0 75 6189.5 76 4118.0 77 4118.0 78 4224.0 79 4330.0 80 3302.5 81 2275.0 82 2275.0 83 2225.0 84 2175.0 85 2175.0 86 1380.0 87 585.0 88 482.5 89 380.0 90 380.0 91 237.0 92 94.0 93 61.5 94 29.0 95 29.0 96 23.0 97 17.0 98 17.0 99 14.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009427079088283614 2 0.0 3 0.0 4 9.819874050295431E-5 5 0.0 6 1.9639748100590862E-4 7 5.891924430177259E-4 8 0.0020621735505620404 9 0.002160372291064995 10 8.837886645265889E-4 11 0.00500813576565067 12 7.855899240236345E-4 13 1.9639748100590862E-4 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 9.819874050295431E-5 22 0.0 23 1.9639748100590862E-4 24 0.0 25 0.0 26 9.819874050295431E-5 27 5.891924430177259E-4 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 1018343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.854999737425686 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4935952164504 48.55160524803489 2 11.556575077073449 13.603244462533961 3 2.816201841120231 4.9724267585900686 4 1.0720872292950558 2.523907743946318 5 0.5324279372633616 1.5668023053916624 6 0.3342858836645684 1.180463735718197 7 0.2056059029524977 0.8470654754997695 8 0.15271828909338553 0.7190587891593045 9 0.10417949894567882 0.5518335944783761 >10 0.5665179761556727 6.313181455993287 >50 0.0699152016952446 2.9445594810397506 >100 0.083819883497979 10.242546898608655 >500 0.009052547094464744 3.4651917216393455 >1k 0.0030175156981549147 2.518112329366458 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 3239 0.318065720489069 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 2377 0.23341840617552237 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 1501 0.14739630949493443 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 1494 0.14670891831141372 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 1452 0.14258457121028967 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG 1407 0.1381656278876567 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA 1292 0.12687277272981698 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC 1256 0.12333761807171062 No Hit ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA 1254 0.12314122059070472 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 1238 0.12157004074265744 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG 1229 0.12068625207813084 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC 1190 0.11685650119851562 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC 1179 0.11577631505298312 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG 1171 0.1149907251289595 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT 1081 0.10615283848369361 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG 1062 0.10428706241413749 No Hit GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG 1062 0.10428706241413749 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1038 0.10193029264206659 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 9.819874050295431E-5 6 0.0 0.0 0.0 0.0 1.9639748100590862E-4 7 0.0 0.0 0.0 0.0 1.9639748100590862E-4 8 0.0 0.0 0.0 0.0 1.9639748100590862E-4 9 0.0 0.0 0.0 0.0 1.9639748100590862E-4 10 0.0 0.0 0.0 0.0 1.9639748100590862E-4 11 0.0 0.0 0.0 0.0 1.9639748100590862E-4 12 0.0 0.0 0.0 9.819874050295431E-5 6.873911835206802E-4 13 0.0 0.0 0.0 9.819874050295431E-5 6.873911835206802E-4 14 0.0 0.0 0.0 9.819874050295431E-5 6.873911835206802E-4 15 0.0 0.0 0.0 9.819874050295431E-5 7.855899240236345E-4 16 0.0 0.0 0.0 1.9639748100590862E-4 7.855899240236345E-4 17 0.0 0.0 0.0 2.9459622150886295E-4 7.855899240236345E-4 18 0.0 0.0 0.0 2.9459622150886295E-4 8.837886645265887E-4 19 0.0 0.0 0.0 2.9459622150886295E-4 8.837886645265887E-4 20 0.0 0.0 0.0 3.9279496201181723E-4 9.81987405029543E-4 21 0.0 0.0 0.0 9.81987405029543E-4 0.0010801861455324974 22 0.0 0.0 0.0 0.0010801861455324974 0.0010801861455324974 23 0.0 0.0 0.0 0.002160372291064995 0.0010801861455324974 24 0.0 0.0 0.0 0.0030441609555915835 0.0010801861455324974 25 0.0 0.0 0.0 0.003535154658106355 0.0010801861455324974 26 0.0 0.0 0.0 0.004222545841627036 0.0010801861455324974 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGCG 140 0.0 21.714186 7 CGCGGTC 140 0.0 21.714186 10 TATAGGG 55 5.8326616E-5 20.363543 2 GTAGCGT 50 7.1953924E-4 19.199911 6 ATCGTTT 275 0.0 16.872648 29 GGTCCTA 185 0.0 16.432356 13 GTATAGG 120 3.103014E-8 16.002285 1 TAGAACC 240 0.0 15.999927 4 TAGGAGT 120 3.108653E-8 15.999927 4 CGGCATC 280 0.0 15.999926 25 ATAGACC 100 1.3140725E-6 15.999926 3 ATAGAAC 270 0.0 15.999926 3 CCAGTCG 285 0.0 15.719226 20 CGTTTAT 285 0.0 15.719226 31 AGAACCG 205 0.0 15.609683 5 GAACCGC 195 0.0 15.589672 6 ATACCGT 175 3.6379788E-12 15.542785 6 ACCGTCG 165 2.910383E-11 15.5150795 8 TCGTTTA 300 0.0 15.466595 30 TTTTCGG 280 0.0 15.4285 29 >>END_MODULE