FastQCFastQC Report
Thu 2 Feb 2017
SRR4062843_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062843_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344518
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36460.2711752464451945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24630.1831883247379358No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22860.1700237557251No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21930.16310677878615235No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19860.14771092688978504No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19740.14681841373637244No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19560.14547964400625354No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19430.14451275475672323No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19200.14280210454601575No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19080.14190959139260315No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18360.13655451247212755No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17360.1291169028603559No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17300.1286706462836496No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17230.1281500136108256No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG16810.1250262175738815No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16540.12301806297870316No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15830.11773736015434526No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC15440.11483669240575432No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15280.11364667486787088No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14560.10829159594739528No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14010.10420091066092087No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13800.10263901264244882No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13650.10152337120068305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4300.021.20861228
CTAGCGG4450.020.49371529
TAGGTCG400.00448664419.9978625
TAGCGGC4500.019.91046330
ACACTTA601.13929826E-418.664676
GGTATAT701.7727274E-518.3035011
CAAGACG5800.017.6532864
CCTAGTA550.001362047517.4598222
GAATTAG652.0851883E-417.2475281
GTGTTAA753.2074815E-517.0832651
AAGACGG5950.016.6704865
TAGCGTA3200.016.498857
ATCGTTT3900.016.40972329
GCGCAAG5700.016.2965371
GTATAGG703.6697148E-416.0155621
AGCGGCG5600.015.99947931
TAATACC600.00244393815.998294
AGCGTAT3350.015.7600948
CGGTCCA5900.015.72830210
GACGGAC5950.015.59555157
Produced by FastQC (version 0.11.5)