##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062843_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1344518
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183833165491276	32.0	32.0	32.0	32.0	32.0
2	30.726112257329394	32.0	32.0	32.0	32.0	32.0
3	30.783894302642285	32.0	32.0	32.0	32.0	32.0
4	30.860750841565526	32.0	32.0	32.0	32.0	32.0
5	30.713570960002023	32.0	32.0	32.0	32.0	32.0
6	34.38986685191273	36.0	36.0	36.0	32.0	36.0
7	34.31309658926099	36.0	36.0	36.0	32.0	36.0
8	34.27047685490265	36.0	36.0	36.0	32.0	36.0
9	34.453557334301216	36.0	36.0	36.0	32.0	36.0
10	34.10458915388266	36.0	36.0	36.0	32.0	36.0
11	34.44851984131116	36.0	36.0	36.0	32.0	36.0
12	34.21070078645284	36.0	36.0	36.0	32.0	36.0
13	34.339932972262176	36.0	36.0	36.0	32.0	36.0
14	34.202565529059484	36.0	36.0	36.0	32.0	36.0
15	34.12510282495288	36.0	36.0	36.0	32.0	36.0
16	34.16153967444095	36.0	36.0	36.0	32.0	36.0
17	34.05219640049445	36.0	36.0	36.0	32.0	36.0
18	34.105573149634296	36.0	36.0	36.0	32.0	36.0
19	34.0815682646123	36.0	36.0	36.0	32.0	36.0
20	34.05639716240318	36.0	36.0	36.0	32.0	36.0
21	34.049829753115986	36.0	36.0	36.0	32.0	36.0
22	34.02723206383254	36.0	36.0	36.0	32.0	36.0
23	33.95921586769385	36.0	36.0	36.0	32.0	36.0
24	33.94304724815882	36.0	36.0	36.0	32.0	36.0
25	33.92268530432467	36.0	36.0	36.0	32.0	36.0
26	33.85789777451845	36.0	36.0	36.0	32.0	36.0
27	33.867142723265886	36.0	36.0	36.0	32.0	36.0
28	33.84220888080338	36.0	36.0	36.0	32.0	36.0
29	33.81363135339207	36.0	36.0	36.0	32.0	36.0
30	33.7842847771469	36.0	36.0	36.0	32.0	36.0
31	33.811374038874895	36.0	36.0	36.0	32.0	36.0
32	33.7609448144242	36.0	36.0	36.0	32.0	36.0
33	33.71012809051273	36.0	36.0	36.0	27.0	36.0
34	33.73061721747124	36.0	36.0	36.0	32.0	36.0
35	33.6818383985934	36.0	36.0	36.0	27.0	36.0
36	33.62981380688098	36.0	36.0	36.0	27.0	36.0
37	33.64090551409502	36.0	36.0	36.0	27.0	36.0
38	33.0105138049472	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	1.0
8	2.0
9	7.0
10	11.0
11	15.0
12	10.0
13	9.0
14	118.0
15	297.0
16	369.0
17	412.0
18	563.0
19	838.0
20	1261.0
21	1763.0
22	2847.0
23	4553.0
24	6932.0
25	10281.0
26	15083.0
27	21780.0
28	30405.0
29	40147.0
30	53377.0
31	71384.0
32	96379.0
33	142831.0
34	301570.0
35	541270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.195379814182836	16.71922575437494	11.08603333152138	25.999361099920844
2	17.471956016675325	19.25743907866008	36.22112812941385	27.049476775250746
3	19.28892399861049	23.280869590443608	27.774393260123613	29.65581315082229
4	12.812872821827984	14.659168330263983	34.95287383337449	37.57508501453354
5	15.41968665280789	35.79997248078303	32.681375812095816	16.098965054313265
6	35.414086736238325	34.62487904542388	16.39671762191961	13.564316596418182
7	31.439394221121788	29.617252887096257	20.246452815397646	18.696900076384306
8	29.445382826346787	31.296685119444735	19.011121499011168	20.24681055519731
9	27.936116693466932	13.673937199612649	18.142689477387368	40.24725662953305
10	16.681108121998438	25.473611556337666	30.26301794930343	27.582262372360468
11	38.70608570956806	20.661527682533354	21.31203263086336	19.320353977035225
12	25.532717231633168	23.09169006489522	27.58705070752524	23.78854199594637
13	29.821934397353044	18.336189762083286	24.99717332571619	26.84470251484748
14	24.423663545812733	19.229141392014412	24.010095232286297	32.337099829886554
15	25.912182594975082	26.333467487861	21.770378531506353	25.98397138565757
16	27.020539564305057	24.812980843972056	22.756272217474063	25.410207374248817
17	24.895387996158462	25.121833464385052	24.28530938744418	25.69746915201231
18	26.297104289751026	23.538403063884733	25.375231416570653	24.789261229793585
19	26.19346565466928	24.188413601863726	24.719875870646028	24.898244872820968
20	26.443286086615014	23.108569269401503	24.25619146226808	26.191953181715398
21	28.221307006458495	23.144140532314413	23.644947193273584	24.989605267953504
22	26.969419503889785	23.119578048212645	24.297261493626614	25.613740954270952
23	25.02789348408212	22.997768521273432	25.100119012198753	26.8742189824457
24	25.490465911001287	24.254389162217127	24.37452299800496	25.880621928776627
25	25.675421350299672	23.595244843261483	24.45605883766307	26.273274968775777
26	25.453403914077168	24.810901927877907	24.942273759257773	24.793420398787152
27	26.43044109869659	23.67231903171171	24.04326711013818	25.853972759453526
28	25.326383186356765	23.648499886941103	25.17970414271603	25.8454127839861
29	24.927419372834947	24.143853575697708	25.22703993775509	25.70168711371225
30	24.872198995442822	24.329086564029176	25.529333798447425	25.26938064208058
31	25.763051745823716	24.372209333721102	23.727255349265157	26.137483571190025
32	25.557282024237836	24.02570016817574	23.605744305938515	26.811273501647914
33	24.875000185956285	23.625448340600506	24.629165978628418	26.870385494814798
34	26.252499345409536	23.85178881721454	24.58998833638809	25.30572350098784
35	26.807109940750497	23.430371601893903	24.894686141268974	24.867832316086634
36	25.09610806950726	24.6808051654368	24.102148298024282	26.120938467031657
37	26.56903641199888	24.401811376443774	24.093493564377056	24.935658647180286
38	25.202872269685116	23.936473272926612	24.675962220757757	26.184692236630514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	93.5
2	126.0
3	126.0
4	365.5
5	605.0
6	605.0
7	798.0
8	991.0
9	1038.5
10	1086.0
11	1086.0
12	1438.5
13	1791.0
14	2315.0
15	2839.0
16	2839.0
17	4089.5
18	5340.0
19	5340.0
20	6202.0
21	7064.0
22	6964.0
23	6864.0
24	6864.0
25	8002.0
26	9140.0
27	9140.0
28	11987.5
29	14835.0
30	18693.0
31	22551.0
32	22551.0
33	30227.5
34	37904.0
35	37904.0
36	42711.5
37	47519.0
38	56053.0
39	64587.0
40	64587.0
41	69309.5
42	74032.0
43	87077.0
44	100122.0
45	100122.0
46	102927.5
47	105733.0
48	105733.0
49	114776.0
50	123819.0
51	126347.5
52	128876.0
53	128876.0
54	124827.5
55	120779.0
56	120779.0
57	118717.0
58	116655.0
59	105486.0
60	94317.0
61	94317.0
62	91305.0
63	88293.0
64	74681.5
65	61070.0
66	61070.0
67	51505.5
68	41941.0
69	41941.0
70	33611.0
71	25281.0
72	19634.5
73	13988.0
74	13988.0
75	10749.5
76	7511.0
77	7511.0
78	7093.0
79	6675.0
80	5259.5
81	3844.0
82	3844.0
83	3906.0
84	3968.0
85	3968.0
86	2713.0
87	1458.0
88	1247.5
89	1037.0
90	1037.0
91	754.5
92	472.0
93	405.5
94	339.0
95	339.0
96	319.5
97	300.0
98	300.0
99	502.5
100	705.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11788611234658071
2	0.05474080674263937
3	0.011974551474952362
4	0.003123796036944095
5	2.2312828835314964E-4
6	3.718804805885827E-4
7	7.437609611771654E-5
8	3.718804805885827E-4
9	2.9750438447086614E-4
10	6.693848650594487E-4
11	0.001785026306825197
12	0.003198172133061811
13	0.01353644949342441
14	0.008999507630243701
15	0.022089700546961813
16	0.010189525168127165
17	0.01970966547119488
18	0.00602446378553504
19	0.008478874957419684
20	0.006396344266123622
21	0.006470720362241338
22	0.0073632335156539365
23	0.008776379341890551
24	0.013685201685659842
25	0.015544604088602755
26	0.017999015260487403
27	0.009297012014714567
28	0.006396344266123622
29	0.011230790513775197
30	0.003346924325297244
31	0.00602446378553504
32	0.006768224746712205
33	0.008627627149655117
34	0.012346431955540945
35	0.015098347511896456
36	0.014726467031307873
37	0.009371388110832284
38	0.005652583304946456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1344518.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.77211084502714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88448551871578	38.26703135500406
2	13.694510650292422	13.632094041095186
3	4.430936058445282	6.6161112194449885
4	1.8379491132549428	3.6591442796977742
5	0.9188039126838939	2.2865405093473705
6	0.5364903095936765	1.602135309382764
7	0.3280645187818143	1.1429924515190264
8	0.22920105754024042	0.9126256353352232
9	0.1654630069793396	0.7411898811714471
>10	0.7742999215272875	6.992751403558158
>50	0.08229347964051806	2.93395803172775
>100	0.09424208193931885	10.362548270592816
>500	0.01590709215601095	5.387047391838882
>1k	0.007353278449476758	5.463830220284481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3646	0.2711752464451945	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2463	0.1831883247379358	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2286	0.1700237557251	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2193	0.16310677878615235	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1986	0.14771092688978504	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1974	0.14681841373637244	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1956	0.14547964400625354	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1943	0.14451275475672323	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1920	0.14280210454601575	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1908	0.14190959139260315	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1836	0.13655451247212755	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1736	0.1291169028603559	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1730	0.1286706462836496	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1723	0.1281500136108256	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1681	0.1250262175738815	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1654	0.12301806297870316	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1583	0.11773736015434526	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1544	0.11483669240575432	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1528	0.11364667486787088	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1456	0.10829159594739528	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1401	0.10420091066092087	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1380	0.10263901264244882	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1365	0.10152337120068305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4875219223543307E-4
2	0.0	0.0	0.0	0.0	1.4875219223543307E-4
3	0.0	0.0	0.0	0.0	1.4875219223543307E-4
4	0.0	0.0	0.0	0.0	2.231282883531496E-4
5	0.0	0.0	0.0	0.0	2.231282883531496E-4
6	0.0	0.0	0.0	0.0	2.9750438447086614E-4
7	0.0	0.0	0.0	0.0	2.9750438447086614E-4
8	0.0	0.0	0.0	0.0	2.9750438447086614E-4
9	0.0	0.0	0.0	0.0	2.9750438447086614E-4
10	0.0	0.0	1.4875219223543307E-4	2.9750438447086614E-4	2.9750438447086614E-4
11	0.0	0.0	1.4875219223543307E-4	2.9750438447086614E-4	2.9750438447086614E-4
12	0.0	0.0	1.4875219223543307E-4	2.9750438447086614E-4	6.693848650594489E-4
13	0.0	0.0	1.4875219223543307E-4	3.7188048058858265E-4	8.925131534125984E-4
14	0.0	0.0	1.4875219223543307E-4	4.462565767062992E-4	8.925131534125984E-4
15	0.0	0.0	1.4875219223543307E-4	5.950087689417323E-4	0.0011900175378834646
16	0.0	0.0	1.4875219223543307E-4	5.950087689417323E-4	0.0013387697301188977
17	0.0	0.0	1.4875219223543307E-4	6.693848650594489E-4	0.0013387697301188977
18	0.0	0.0	1.4875219223543307E-4	8.181370572948819E-4	0.0015618980184720472
19	0.0	0.0	1.4875219223543307E-4	8.181370572948819E-4	0.0015618980184720472
20	0.0	0.0	1.4875219223543307E-4	0.0010412653456480314	0.0017850263068251969
21	0.0	0.0	1.4875219223543307E-4	0.001115641441765748	0.00193377849906063
22	0.0	0.0	1.4875219223543307E-4	0.0014131458262366143	0.0020081545951783466
23	0.0	0.0	1.4875219223543307E-4	0.0018594024029429134	0.002231282883531496
24	0.0	0.0	1.4875219223543307E-4	0.002380035075766929	0.002231282883531496
25	0.0	0.0	1.4875219223543307E-4	0.0028262916524732285	0.002231282883531496
26	0.0	0.0	1.4875219223543307E-4	0.0031237960369440943	0.0026031633641200786
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	430	0.0	21.208612	28
CTAGCGG	445	0.0	20.493715	29
TAGGTCG	40	0.004486644	19.997862	5
TAGCGGC	450	0.0	19.910463	30
ACACTTA	60	1.13929826E-4	18.66467	6
GGTATAT	70	1.7727274E-5	18.303501	1
CAAGACG	580	0.0	17.653286	4
CCTAGTA	55	0.0013620475	17.459822	2
GAATTAG	65	2.0851883E-4	17.247528	1
GTGTTAA	75	3.2074815E-5	17.083265	1
AAGACGG	595	0.0	16.670486	5
TAGCGTA	320	0.0	16.49885	7
ATCGTTT	390	0.0	16.409723	29
GCGCAAG	570	0.0	16.296537	1
GTATAGG	70	3.6697148E-4	16.015562	1
AGCGGCG	560	0.0	15.999479	31
TAATACC	60	0.002443938	15.99829	4
AGCGTAT	335	0.0	15.760094	8
CGGTCCA	590	0.0	15.728302	10
GACGGAC	595	0.0	15.5955515	7
>>END_MODULE