FastQCFastQC Report
Thu 2 Feb 2017
SRR4062843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344518
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41830.31111521006040826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30660.2280371106969189No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24820.18460147056417242No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24050.17887451116310826No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22770.16935437086004054No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20500.1524709970413189No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20240.15053721854225827No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19980.14860344004319764No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19970.14852906394707993No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19110.1421327196809563No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18860.1402733172780134No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18640.13863704316342362No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18080.13447198178083147No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17630.13112505745553424No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17320.12881939847588503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17040.12673686778458898No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15700.11677047090481495No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15410.11461356411740119No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14920.11096913540763306No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14800.11007662225422046No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14520.1079940915629244No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG14370.10687845012115867No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT14280.10620906525609922No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14130.10509342381433345No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG14100.10487029552598032No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA13880.10323402141139056No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT13850.10301089312303739No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC13620.10130024291232992No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC13620.10130024291232992No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13600.10115149072009448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT350.002067056522.85650615
ATCGTTT3250.019.69175729
CATCGTT3350.019.58154328
CGTCTTA852.3974644E-718.82300415
CTAGCGG4950.017.77728329
TCTAGCG5050.017.42525728
TAGCGGC5150.017.39757330
TACCGTC4150.017.350857
CTCGACG652.1011858E-417.23028813
TCGTTTA3850.016.62291330
CGGTCCA5800.016.27601210
CAAGACG5850.016.13754
AAGACGG6000.016.0013375
GTTTTCG5150.015.84421928
ATACCGT4450.015.8215486
ACCGTCG4350.015.8168278
CGTTTAT4150.015.806787531
TTTCGGA5400.015.70326730
CTATTCC5150.015.5347034
TTCGGAA5400.015.40697931
Produced by FastQC (version 0.11.5)