FastQCFastQC Report
Thu 2 Feb 2017
SRR4062842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818479
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23940.2924937597665914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16380.20012730931398362No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10500.1282867367397331No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10150.12401051218174199No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10120.12364397864819988No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9780.11948993193472282No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9480.11582459659930186No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9390.1147249959986756No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9350.11423628462061947No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9160.11191490557485287No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9090.11105966066325465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8770.10714996963880564No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8740.10678343610526354No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8520.10409552352595484No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8380.1023850337027584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA2450.018.94029410
AAATCGT450.00884095717.78028912
GTTATTC1453.6379788E-1217.654433
TACCGTC2450.017.6308357
CCGTCGT2500.017.2813859
ACCGTCG2500.017.2782178
ACGTTTT652.1001464E-417.23004529
ATACCGT2550.016.9363256
GTCGTAG2600.016.61773311
CCGATAA908.568228E-616.0012849
CGATAAC908.568228E-616.00128410
CGTTTTT703.6959536E-415.99932830
GTCCTAT2450.015.68335151
TATTAGC2150.015.6329832
CGTTATT1652.910383E-1115.5201892
CGACGGT3550.015.3223637
TCCAATA2300.015.3093192
GTAGAAC1807.2759576E-1215.1104763
ACGACGG3600.015.1067836
GATATAT859.3763665E-515.0683191
Produced by FastQC (version 0.11.5)