##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062842_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	818479
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255477538214176	32.0	32.0	32.0	32.0	32.0
2	30.90526085580693	32.0	32.0	32.0	32.0	32.0
3	30.964452356138644	32.0	32.0	32.0	32.0	32.0
4	31.01991376687734	32.0	32.0	32.0	32.0	32.0
5	30.953706814713634	32.0	32.0	32.0	32.0	32.0
6	34.60599477811893	36.0	36.0	36.0	32.0	36.0
7	34.52908749033268	36.0	36.0	36.0	32.0	36.0
8	34.5139337722776	36.0	36.0	36.0	32.0	36.0
9	34.6220281766545	36.0	36.0	36.0	32.0	36.0
10	34.3860538877601	36.0	36.0	36.0	32.0	36.0
11	34.633054727121895	36.0	36.0	36.0	32.0	36.0
12	34.4612519075016	36.0	36.0	36.0	32.0	36.0
13	34.53588546560144	36.0	36.0	36.0	32.0	36.0
14	34.43878951078769	36.0	36.0	36.0	32.0	36.0
15	34.397830610192806	36.0	36.0	36.0	32.0	36.0
16	34.39996261357958	36.0	36.0	36.0	32.0	36.0
17	34.33085393760866	36.0	36.0	36.0	32.0	36.0
18	34.349453070879036	36.0	36.0	36.0	32.0	36.0
19	34.35106826198351	36.0	36.0	36.0	32.0	36.0
20	34.32556485871965	36.0	36.0	36.0	32.0	36.0
21	34.28715092262599	36.0	36.0	36.0	32.0	36.0
22	34.27242849236205	36.0	36.0	36.0	32.0	36.0
23	34.22835405673206	36.0	36.0	36.0	32.0	36.0
24	34.21159370002162	36.0	36.0	36.0	32.0	36.0
25	34.20175105286758	36.0	36.0	36.0	32.0	36.0
26	34.1497900373742	36.0	36.0	36.0	32.0	36.0
27	34.156600230427415	36.0	36.0	36.0	32.0	36.0
28	34.142379951104424	36.0	36.0	36.0	32.0	36.0
29	34.10411873731641	36.0	36.0	36.0	32.0	36.0
30	34.07605204287465	36.0	36.0	36.0	32.0	36.0
31	34.09089542920466	36.0	36.0	36.0	32.0	36.0
32	34.04538418212318	36.0	36.0	36.0	32.0	36.0
33	34.00589141566247	36.0	36.0	36.0	32.0	36.0
34	34.00237269374046	36.0	36.0	36.0	32.0	36.0
35	33.9470640053074	36.0	36.0	36.0	32.0	36.0
36	33.91240581615411	36.0	36.0	36.0	32.0	36.0
37	33.912955616454425	36.0	36.0	36.0	32.0	36.0
38	33.314400247287956	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	3.0
10	8.0
11	12.0
12	4.0
13	2.0
14	123.0
15	269.0
16	335.0
17	325.0
18	426.0
19	549.0
20	758.0
21	1041.0
22	1582.0
23	2338.0
24	3355.0
25	5043.0
26	7343.0
27	10867.0
28	14862.0
29	20523.0
30	27760.0
31	37150.0
32	51250.0
33	76862.0
34	174494.0
35	381191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.730067983276946	17.11801299975292	11.304329744579562	25.84758927239057
2	16.772771612770732	19.955675514450075	37.24662554488802	26.024927327891177
3	18.853886261078248	24.17401143474388	28.446094310365684	28.526007993812186
4	12.62147784503129	15.437124753501077	35.99006429131713	35.9513331101505
5	14.814466380851263	36.373410615081426	33.36843918644018	15.443683817627129
6	34.00193285822326	35.435449010784666	17.063032925787773	13.499585205204301
7	30.123436276312525	30.494979101479696	21.00542591807487	18.376158704132912
8	28.225995630904265	32.50260239762681	19.714811430023605	19.55659054144532
9	27.852374412933305	14.009451712695304	18.614840264100597	39.5233336102708
10	16.153598037323118	26.57494312586594	31.304720736394803	25.966738100416144
11	37.70824296998613	21.132377467301193	21.932177089760586	19.227202472952087
12	24.962154302924414	23.83515281842007	28.614645887678613	22.588046990976903
13	29.921328158604776	19.29036483791499	25.015885895280178	25.772421108200056
14	23.963166300292897	19.816931233740764	25.052023446292132	31.16787901967421
15	25.4753367503318	26.860345463922314	22.530069830934345	25.13424795481154
16	25.90189415184741	25.588340597853517	23.777892641511507	24.731872608787565
17	24.110244938511794	25.55582576773186	25.239921934480204	25.094007359276144
18	25.039588088384075	24.58175917734975	26.40611025038244	23.97254248388374
19	25.637905185717376	24.819744361398353	25.500078810294458	24.042271642589817
20	25.958133658515127	23.905382850244496	25.058955491963726	25.07752799927665
21	27.2714943073656	24.09942988125988	24.14366112513532	24.485414686239196
22	25.992206773435584	24.276670540064003	24.902890140920793	24.828232545579624
23	24.39286059040527	23.87012501420477	25.55025922943546	26.186755165954494
24	24.805765804516938	24.960346652133218	25.160263068497034	25.073624474852814
25	25.123456714688814	24.363347073150166	25.173682609009134	25.339513603151886
26	24.53360511696228	25.36166531019262	25.694795783443197	24.409933789401904
27	25.467564364178447	24.62969977639023	24.986742262246604	24.915993597184716
28	24.492778237330302	24.64086683145392	25.67553202538033	25.190822905835446
29	24.36975714067512	24.86427371315106	25.713639835038947	25.05232931113487
30	24.457053629355944	25.19833197100376	25.948163054355117	24.39645134528518
31	25.27800416166212	24.933683437537802	24.465954646924633	25.32235775387544
32	24.56372722718442	25.074595862709398	24.492980291052163	25.86869661905402
33	24.290867235097032	24.52657277546023	25.32655420249904	25.856005786943697
34	25.17638979517522	24.790617309294955	25.502775069101784	24.530217826428043
35	25.87639813716133	24.51861799953808	25.36571834280191	24.23926552049868
36	24.46152793358213	25.567799944765827	24.843890147543668	25.12678197410837
37	25.524021789335542	25.235525708043433	24.87799096265558	24.362461539965445
38	24.45639689746685	25.08577419149882	25.311817288753563	25.14601162228076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	488.0
1	360.5
2	233.0
3	233.0
4	468.0
5	703.0
6	703.0
7	824.5
8	946.0
9	882.0
10	818.0
11	818.0
12	1086.0
13	1354.0
14	1658.5
15	1963.0
16	1963.0
17	2874.0
18	3785.0
19	3785.0
20	4543.5
21	5302.0
22	5430.5
23	5559.0
24	5559.0
25	6638.5
26	7718.0
27	7718.0
28	10009.0
29	12300.0
30	15262.5
31	18225.0
32	18225.0
33	22834.0
34	27443.0
35	27443.0
36	30616.5
37	33790.0
38	39006.0
39	44222.0
40	44222.0
41	47140.0
42	50058.0
43	56774.0
44	63490.0
45	63490.0
46	66018.0
47	68546.0
48	68546.0
49	72886.5
50	77227.0
51	77287.0
52	77347.0
53	77347.0
54	73810.5
55	70274.0
56	70274.0
57	68438.0
58	66602.0
59	59402.5
60	52203.0
61	52203.0
62	49214.0
63	46225.0
64	38104.0
65	29983.0
66	29983.0
67	24908.5
68	19834.0
69	19834.0
70	15984.0
71	12134.0
72	9443.5
73	6753.0
74	6753.0
75	5094.5
76	3436.0
77	3436.0
78	3465.5
79	3495.0
80	2676.0
81	1857.0
82	1857.0
83	1948.0
84	2039.0
85	2039.0
86	1378.0
87	717.0
88	599.0
89	481.0
90	481.0
91	346.0
92	211.0
93	192.5
94	174.0
95	174.0
96	164.0
97	154.0
98	154.0
99	272.0
100	390.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11301450617547916
2	0.05143687254040727
3	0.011729073073347026
4	0.0020770233567385357
5	2.4435568902806304E-4
6	2.4435568902806304E-4
7	0.0
8	3.6653353354209456E-4
9	3.6653353354209456E-4
10	6.108892225701576E-4
11	0.0029322682683367565
12	0.0029322682683367565
13	0.017471431765506504
14	0.011973428762375088
15	0.02651259225954484
16	0.014416985652655718
17	0.023458146146694052
18	0.006231070070215608
19	0.007086314981813829
20	0.007941559893412048
21	0.0064754257592436705
22	0.009285516183066394
23	0.0112403616952909
24	0.01673836469842232
25	0.021503300634469546
26	0.022114189857039706
27	0.010873828161748806
28	0.006108892225701576
29	0.012706495829459277
30	0.003420979646392882
31	0.006841959292785764
32	0.008430271271468174
33	0.010385116783692678
34	0.014783519186197813
35	0.01930409943321698
36	0.01771578745453457
37	0.011851250917861057
38	0.006231070070215608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	818479.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.38974728951386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.75047370487927	51.41420416119614
2	10.918381213475987	13.405533270117335
3	2.593830829605839	4.777038574237578
4	0.9472945177622868	2.3261668421667476
5	0.4779648662749686	1.4671071176943307
6	0.27963180589283854	1.0299915538723052
7	0.1688674178594972	0.7256709675479092
8	0.1251853062477513	0.6148075451927882
9	0.08263631622606202	0.4565720313048872
>10	0.4910177305490889	5.842449030045124
>50	0.0724519034881572	3.1958932258650083
>100	0.08345824227721539	10.482493461446241
>500	0.007805447113246676	3.3886232468122524
>1k	0.0010006983478521379	0.8734489725013888
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2394	0.2924937597665914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1638	0.20012730931398362	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1050	0.1282867367397331	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1015	0.12401051218174199	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1012	0.12364397864819988	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	978	0.11948993193472282	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	948	0.11582459659930186	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	939	0.1147249959986756	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	935	0.11423628462061947	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	916	0.11191490557485287	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	909	0.11105966066325465	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	877	0.10714996963880564	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	874	0.10678343610526354	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	852	0.10409552352595484	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	838	0.1023850337027584	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.2217784451403152E-4
13	0.0	0.0	0.0	0.0	1.2217784451403152E-4
14	0.0	0.0	0.0	0.0	1.2217784451403152E-4
15	0.0	0.0	0.0	0.0	1.2217784451403152E-4
16	0.0	0.0	0.0	0.0	1.2217784451403152E-4
17	0.0	0.0	0.0	0.0	1.2217784451403152E-4
18	0.0	0.0	0.0	0.0	1.2217784451403152E-4
19	0.0	0.0	0.0	2.4435568902806304E-4	1.2217784451403152E-4
20	0.0	0.0	0.0	2.4435568902806304E-4	1.2217784451403152E-4
21	0.0	0.0	0.0	6.108892225701576E-4	1.2217784451403152E-4
22	0.0	0.0	0.0	0.0010996006006262836	1.2217784451403152E-4
23	0.0	0.0	0.0	0.0020770233567385357	1.2217784451403152E-4
24	0.0	0.0	0.0	0.0034209796463928826	1.2217784451403152E-4
25	0.0	0.0	0.0	0.003909691024449009	1.2217784451403152E-4
26	0.0	1.2217784451403152E-4	0.0	0.004887113780561261	1.2217784451403152E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	245	0.0	18.940294	10
AAATCGT	45	0.008840957	17.780289	12
GTTATTC	145	3.6379788E-12	17.65443	3
TACCGTC	245	0.0	17.630835	7
CCGTCGT	250	0.0	17.281385	9
ACCGTCG	250	0.0	17.278217	8
ACGTTTT	65	2.1001464E-4	17.230045	29
ATACCGT	255	0.0	16.936325	6
GTCGTAG	260	0.0	16.617733	11
CCGATAA	90	8.568228E-6	16.001284	9
CGATAAC	90	8.568228E-6	16.001284	10
CGTTTTT	70	3.6959536E-4	15.999328	30
GTCCTAT	245	0.0	15.6833515	1
TATTAGC	215	0.0	15.632983	2
CGTTATT	165	2.910383E-11	15.520189	2
CGACGGT	355	0.0	15.322363	7
TCCAATA	230	0.0	15.309319	2
GTAGAAC	180	7.2759576E-12	15.110476	3
ACGACGG	360	0.0	15.106783	6
GATATAT	85	9.3763665E-5	15.068319	1
>>END_MODULE