FastQCFastQC Report
Thu 2 Feb 2017
SRR4062842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818479
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29260.3574923730480562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22250.27184570404372016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12600.1539440840876797No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10690.1306081157854997No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10510.12840891458424714No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10240.12511011278236828No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10030.12254437804757361No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9750.11912339840118072No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9640.11777944211152638No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9630.11765726426701235No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9110.11130401635228271No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.11081530497422658No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8960.10947134868457223No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8890.10861610377297401No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8770.10714996963880564No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8280.10116325525761809No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8250.10079672172407599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG555.8298072E-520.3640251
ATACGTC400.00447873320.0028255
GTTAATA450.00884407317.7792022
TAGGACG550.001363458117.4559444
CGAGCCG2600.017.23004515
AACCGCG1700.016.943577
AGGCCCG2850.016.84242610
CGCGGTC1750.016.45745810
TAAGACC805.613837E-516.0012844
GTTCAAA3500.016.0003071
CCCTATA600.00244078916.0003051
CAAATCG1304.796675E-915.99932813
TACCGCA3100.015.99932828
TACCGTC2450.015.6756847
GCCGCCT3200.015.49934818
AAATCGC1357.90169E-915.4067614
GTAGGAC1255.1250026E-815.3612323
GCAGCTA3250.015.26089832
ACCGTCG2550.015.0609518
ATCTAAT859.420459E-515.0600322
Produced by FastQC (version 0.11.5)