##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	818479
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18777390745517	32.0	32.0	32.0	32.0	32.0
2	31.30635239266982	32.0	32.0	32.0	32.0	32.0
3	31.379026218143654	32.0	32.0	32.0	32.0	32.0
4	31.47908376390842	32.0	32.0	32.0	32.0	32.0
5	31.40532011206152	32.0	32.0	32.0	32.0	32.0
6	34.941729720615925	36.0	36.0	36.0	36.0	36.0
7	34.964740695851695	36.0	36.0	36.0	36.0	36.0
8	34.910125977575476	36.0	36.0	36.0	36.0	36.0
9	35.01462346620988	36.0	36.0	36.0	36.0	36.0
10	34.85544650504167	36.0	36.0	36.0	32.0	36.0
11	35.03048215042781	36.0	36.0	36.0	36.0	36.0
12	34.924925379881465	36.0	36.0	36.0	32.0	36.0
13	34.985178605682	36.0	36.0	36.0	36.0	36.0
14	34.92164490475626	36.0	36.0	36.0	32.0	36.0
15	34.90012816455889	36.0	36.0	36.0	32.0	36.0
16	34.906717215713535	36.0	36.0	36.0	32.0	36.0
17	34.877930893767584	36.0	36.0	36.0	32.0	36.0
18	34.87278965007044	36.0	36.0	36.0	32.0	36.0
19	34.86621037314335	36.0	36.0	36.0	32.0	36.0
20	34.83604466333284	36.0	36.0	36.0	32.0	36.0
21	34.83248317916526	36.0	36.0	36.0	32.0	36.0
22	34.81927453239484	36.0	36.0	36.0	32.0	36.0
23	34.757650471178856	36.0	36.0	36.0	32.0	36.0
24	34.73803848357746	36.0	36.0	36.0	32.0	36.0
25	34.71820779763439	36.0	36.0	36.0	32.0	36.0
26	34.65297704644835	36.0	36.0	36.0	32.0	36.0
27	34.63912452243735	36.0	36.0	36.0	32.0	36.0
28	34.607845772463314	36.0	36.0	36.0	32.0	36.0
29	34.56974827698695	36.0	36.0	36.0	32.0	36.0
30	34.54369140808744	36.0	36.0	36.0	32.0	36.0
31	34.547459372812256	36.0	36.0	36.0	32.0	36.0
32	34.50505266475988	36.0	36.0	36.0	32.0	36.0
33	34.46632106627048	36.0	36.0	36.0	32.0	36.0
34	34.460206065152555	36.0	36.0	36.0	32.0	36.0
35	34.42183855663981	36.0	36.0	36.0	32.0	36.0
36	34.39846715676273	36.0	36.0	36.0	32.0	36.0
37	34.38421633297861	36.0	36.0	36.0	32.0	36.0
38	33.943862945781134	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	8.0
21	11.0
22	43.0
23	203.0
24	488.0
25	1246.0
26	2773.0
27	5482.0
28	9669.0
29	14981.0
30	22727.0
31	32763.0
32	47296.0
33	75277.0
34	184500.0
35	421011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.014821784234904	18.431065873238918	12.003934554430039	26.550177788096143
2	15.713292582949592	21.025585262419682	36.54986871990607	26.711253434724654
3	17.899909466217213	24.912062496411025	28.83873624124749	28.349291796124277
4	12.063107300248388	16.3428750157304	35.92798349132965	35.66603419269156
5	14.163955336667158	37.04468899018789	33.06938846323486	15.72196720991009
6	33.909036040064606	35.87903401190991	16.737896437045347	13.474033510980135
7	29.708018316969586	30.747389049892725	21.093593459062944	18.450999174074745
8	28.031302690418592	32.66952569459717	19.642988050729418	19.65618356425482
9	27.698969505475034	13.75919124385112	18.442846285992164	40.09899296468167
10	15.79001384285126	26.806259949931153	31.57049774396956	25.833228463248027
11	37.71475280441718	21.010066699982037	22.067442857823593	19.20773763777719
12	24.824612632777583	23.8071093278467	28.58258173136838	22.785696308007342
13	29.804869287713643	19.316364418303753	24.970157988556796	25.90860830542581
14	23.869274593483766	19.763365950745225	25.161549654908676	31.205809800862333
15	25.37401692651858	26.94669014110319	22.457265244435103	25.222027687943122
16	25.779769548149677	25.460885373968058	23.852902762318887	24.90644231556338
17	23.985832257150154	25.632667423354782	25.256604017940592	25.124896301554468
18	25.046458125376457	24.43825681538561	26.45113680375428	24.064148255483648
19	25.645496097028758	24.778033400979133	25.59772455982377	23.978745942168338
20	25.945320527466194	23.837997065288175	25.29777795154182	24.91890445570381
21	27.27913605602587	24.058528074636	24.31913341698443	24.343202452353697
22	25.82277614941862	24.202575753318044	25.109868426679245	24.864779670584095
23	24.394454578442645	23.79138326428232	25.61000492377917	26.204157233495874
24	24.7046045164262	24.8073560836625	25.522829541136673	24.965209858774628
25	25.031918961879292	24.132690026256018	25.402484364290352	25.432906647574345
26	24.73820342366756	25.042059722973953	25.816300723659374	24.403436129699113
27	25.573288990920968	24.32304310800888	24.98769058216521	25.115977318904942
28	24.41064462252544	24.475276702273362	25.974276676616014	25.13980199858518
29	24.49238160050533	24.53966442633226	25.88924089683425	25.078713076328164
30	24.405879686589394	24.731605820063802	26.18906532727168	24.673449166075123
31	25.053544440358273	24.607717485726575	24.818107733979737	25.52063033993542
32	24.673204810386093	24.798803634546516	24.809310929174725	25.71868062589266
33	24.37729007097311	24.13281220410053	25.63413355748895	25.855764167437407
34	25.15006493752436	24.51254094485014	25.73346414507886	24.603929972546638
35	25.94165519213077	24.21210562519014	25.618128259857613	24.22811092282148
36	24.600753348589276	25.124529768020924	25.12575154646607	25.148965336923734
37	25.464184175769933	24.90668667125241	25.072970717636007	24.55615843534165
38	24.629831675583613	24.677847568477628	25.52942714474043	25.162893611198335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	186.5
2	124.0
3	124.0
4	441.5
5	759.0
6	759.0
7	850.0
8	941.0
9	896.0
10	851.0
11	851.0
12	1133.5
13	1416.0
14	1736.5
15	2057.0
16	2057.0
17	3209.0
18	4361.0
19	4361.0
20	4903.5
21	5446.0
22	5622.5
23	5799.0
24	5799.0
25	6699.0
26	7599.0
27	7599.0
28	9874.5
29	12150.0
30	15122.5
31	18095.0
32	18095.0
33	22958.5
34	27822.0
35	27822.0
36	30845.5
37	33869.0
38	39143.5
39	44418.0
40	44418.0
41	47342.0
42	50266.0
43	56702.5
44	63139.0
45	63139.0
46	66027.5
47	68916.0
48	68916.0
49	73210.5
50	77505.0
51	77463.5
52	77422.0
53	77422.0
54	73831.5
55	70241.0
56	70241.0
57	68436.0
58	66631.0
59	59432.0
60	52233.0
61	52233.0
62	49233.5
63	46234.0
64	38012.0
65	29790.0
66	29790.0
67	25012.0
68	20234.0
69	20234.0
70	16196.0
71	12158.0
72	9485.5
73	6813.0
74	6813.0
75	5062.5
76	3312.0
77	3312.0
78	3293.0
79	3274.0
80	2542.0
81	1810.0
82	1810.0
83	1762.5
84	1715.0
85	1715.0
86	1064.5
87	414.0
88	366.5
89	319.0
90	319.0
91	185.5
92	52.0
93	41.0
94	30.0
95	30.0
96	17.5
97	5.0
98	5.0
99	7.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010873828161748806
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2217784451403152E-4
7	3.6653353354209456E-4
8	0.0023213790457665986
9	0.004520580247019166
10	7.330670670841891E-4
11	0.004642758091533197
12	6.108892225701576E-4
13	2.4435568902806304E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.4435568902806304E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	818479.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.795573225382334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.73602242949404	49.472478875596856
2	11.56389406952921	13.829393492101968
3	2.799103864312189	5.0212206025179045
4	1.0548149567154266	2.522930599340232
5	0.4699905795585312	1.4051678057616015
6	0.28870941841442294	1.03581271017943
7	0.1830995107080251	0.7663978140051374
8	0.12955690526733	0.6197543532613241
9	0.09765981635063672	0.52556622297947
>10	0.5008045280617873	5.811169177268171
>50	0.07985838132156366	3.394661497627447
>100	0.0868369862438323	10.911792182594187
>500	0.00821153533874852	3.3876246321255867
>1k	0.0014370186842809908	1.2960300346407923
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2926	0.3574923730480562	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2225	0.27184570404372016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1260	0.1539440840876797	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1069	0.1306081157854997	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1051	0.12840891458424714	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1024	0.12511011278236828	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1003	0.12254437804757361	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	975	0.11912339840118072	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	964	0.11777944211152638	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	963	0.11765726426701235	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	911	0.11130401635228271	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.11081530497422658	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	896	0.10947134868457223	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	889	0.10861610377297401	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	877	0.10714996963880564	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	828	0.10116325525761809	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	825	0.10079672172407599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.2217784451403152E-4
6	0.0	0.0	0.0	0.0	1.2217784451403152E-4
7	0.0	0.0	0.0	0.0	1.2217784451403152E-4
8	0.0	0.0	0.0	0.0	1.2217784451403152E-4
9	0.0	0.0	0.0	0.0	1.2217784451403152E-4
10	0.0	0.0	0.0	0.0	1.2217784451403152E-4
11	0.0	0.0	0.0	0.0	1.2217784451403152E-4
12	0.0	0.0	0.0	0.0	2.4435568902806304E-4
13	0.0	0.0	0.0	0.0	2.4435568902806304E-4
14	0.0	0.0	0.0	0.0	2.4435568902806304E-4
15	0.0	0.0	0.0	0.0	2.4435568902806304E-4
16	0.0	0.0	0.0	0.0	2.4435568902806304E-4
17	0.0	0.0	0.0	1.2217784451403152E-4	2.4435568902806304E-4
18	0.0	0.0	0.0	1.2217784451403152E-4	2.4435568902806304E-4
19	0.0	0.0	0.0	2.4435568902806304E-4	2.4435568902806304E-4
20	0.0	0.0	0.0	2.4435568902806304E-4	2.4435568902806304E-4
21	0.0	0.0	0.0	4.887113780561261E-4	2.4435568902806304E-4
22	0.0	0.0	0.0	9.774227561122522E-4	2.4435568902806304E-4
23	0.0	0.0	0.0	0.0018326676677104727	2.4435568902806304E-4
24	0.0	0.0	0.0	0.003054446112850788	2.4435568902806304E-4
25	0.0	0.0	0.0	0.003543157490906914	2.4435568902806304E-4
26	0.0	0.0	0.0	0.004520580247019166	2.4435568902806304E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	55	5.8298072E-5	20.364025	1
ATACGTC	40	0.004478733	20.002825	5
GTTAATA	45	0.008844073	17.779202	2
TAGGACG	55	0.0013634581	17.455944	4
CGAGCCG	260	0.0	17.230045	15
AACCGCG	170	0.0	16.94357	7
AGGCCCG	285	0.0	16.842426	10
CGCGGTC	175	0.0	16.457458	10
TAAGACC	80	5.613837E-5	16.001284	4
GTTCAAA	350	0.0	16.000307	1
CCCTATA	60	0.002440789	16.000305	1
CAAATCG	130	4.796675E-9	15.999328	13
TACCGCA	310	0.0	15.999328	28
TACCGTC	245	0.0	15.675684	7
GCCGCCT	320	0.0	15.499348	18
AAATCGC	135	7.90169E-9	15.40676	14
GTAGGAC	125	5.1250026E-8	15.361232	3
GCAGCTA	325	0.0	15.260898	32
ACCGTCG	255	0.0	15.060951	8
ATCTAAT	85	9.420459E-5	15.060032	2
>>END_MODULE