FastQCFastQC Report
Thu 2 Feb 2017
SRR4062841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765198
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28010.15867908302638004No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26140.1480853705929873No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24420.13834142118901108No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24290.1376049598968501No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23310.13205317477132877No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23120.13097680826740118No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21810.12355554447716346No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21660.12270578144774694No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21180.11998653975361404No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20350.11528451765750924No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20060.11364164246730395No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19560.11080909903591551No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19350.10961943079473237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19220.10888296950257138No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18720.10605042607118297No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17720.10038533920840609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4500.019.19904728
CTAGCGG4550.018.9880729
GTATCAA24250.017.4995331
GTATTAA1900.016.8565521
AAGACGG6150.016.6472235
CAAGACG6200.016.513444
TAGCGGC5450.016.44001830
CGACCCG4250.016.1851395
GTCCTAT6250.016.1418341
CGTACAC805.6259807E-515.9992073
ATACCGT5000.015.9969416
TACCGTC5250.015.5407667
AAACGCT5000.015.35923929
CTTACAC1901.8189894E-1215.1571433
AATAGCG3800.015.1549955
GTTATTC3600.015.1103623
AACGCTT4950.014.86837230
GCGCAAG7050.014.7644261
CGCAAGA6950.014.7389292
TAAACGC5000.014.7192728
Produced by FastQC (version 0.11.5)