##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062841_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1765198
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277470855960633	32.0	32.0	32.0	32.0	32.0
2	30.893829474087326	32.0	32.0	32.0	32.0	32.0
3	30.93478352003571	32.0	32.0	32.0	32.0	32.0
4	31.0125708277485	32.0	32.0	32.0	32.0	32.0
5	30.904958537229252	32.0	32.0	32.0	32.0	32.0
6	34.56559660729278	36.0	36.0	36.0	32.0	36.0
7	34.494859500180716	36.0	36.0	36.0	32.0	36.0
8	34.4867850518752	36.0	36.0	36.0	32.0	36.0
9	34.63253244111992	36.0	36.0	36.0	32.0	36.0
10	34.3420358509357	36.0	36.0	36.0	32.0	36.0
11	34.60239814457075	36.0	36.0	36.0	32.0	36.0
12	34.41912068787751	36.0	36.0	36.0	32.0	36.0
13	34.52405452532804	36.0	36.0	36.0	32.0	36.0
14	34.420607773178986	36.0	36.0	36.0	32.0	36.0
15	34.35620649921425	36.0	36.0	36.0	32.0	36.0
16	34.37478685110679	36.0	36.0	36.0	32.0	36.0
17	34.29864298509289	36.0	36.0	36.0	32.0	36.0
18	34.316326553735045	36.0	36.0	36.0	32.0	36.0
19	34.30916645044918	36.0	36.0	36.0	32.0	36.0
20	34.294591314968635	36.0	36.0	36.0	32.0	36.0
21	34.27853022720397	36.0	36.0	36.0	32.0	36.0
22	34.25556566458833	36.0	36.0	36.0	32.0	36.0
23	34.206221624996175	36.0	36.0	36.0	32.0	36.0
24	34.18513050660606	36.0	36.0	36.0	32.0	36.0
25	34.16872498156014	36.0	36.0	36.0	32.0	36.0
26	34.113714155579146	36.0	36.0	36.0	32.0	36.0
27	34.11765139094878	36.0	36.0	36.0	32.0	36.0
28	34.107792440281486	36.0	36.0	36.0	32.0	36.0
29	34.076214113091	36.0	36.0	36.0	32.0	36.0
30	34.04742640768911	36.0	36.0	36.0	32.0	36.0
31	34.06051615739424	36.0	36.0	36.0	32.0	36.0
32	34.02160890732937	36.0	36.0	36.0	32.0	36.0
33	33.99147744332364	36.0	36.0	36.0	32.0	36.0
34	34.005027764590714	36.0	36.0	36.0	32.0	36.0
35	33.96301661343374	36.0	36.0	36.0	32.0	36.0
36	33.91935522247363	36.0	36.0	36.0	32.0	36.0
37	33.927130554192786	36.0	36.0	36.0	32.0	36.0
38	33.331213268993054	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	3.0
8	4.0
9	12.0
10	14.0
11	25.0
12	11.0
13	9.0
14	135.0
15	328.0
16	489.0
17	503.0
18	747.0
19	902.0
20	1345.0
21	1919.0
22	3080.0
23	4611.0
24	7026.0
25	10900.0
26	15932.0
27	23533.0
28	33193.0
29	45372.0
30	61293.0
31	83562.0
32	115065.0
33	175089.0
34	390528.0
35	789565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53025189588379	17.045370753759155	11.294986472153054	26.129390878204
2	17.23615669336417	19.38232976799814	36.71076572516877	26.67074781346892
3	18.965018070852246	23.200322720095276	28.2971019214197	29.537557287632783
4	12.88104539912891	14.988068962079016	35.50900096536033	36.621884673431744
5	15.164338877199901	35.90472208720401	33.286369656819595	15.644569378776495
6	34.92808139633694	34.98257977894731	16.56246636339431	13.526872461321442
7	30.978751946666577	29.9939893394335	20.61775541200217	18.409503301897747
8	28.609716704235865	32.144922889189644	19.348205034367464	19.897155372207024
9	27.627898123379076	14.07907572765373	18.458537701805014	39.83448844716218
10	16.345001212335454	25.881379207200595	30.85620341855938	26.917416161904566
11	38.100104806956914	20.856301164207007	21.876781010112456	19.166813018723623
12	25.085673139622433	23.40722175420746	28.055410933643188	23.451694172526924
13	29.493707678892466	18.763247700049124	25.13970816956376	26.60333645149465
14	23.986028447350304	19.53088217380589	24.38976439293717	32.093324985906634
15	25.57471557457958	26.637450659740047	21.838001888047618	25.949831877632757
16	26.628751373239577	25.27757994818092	23.038526629374605	25.055142049204893
17	24.744437575679964	25.431471419675404	24.565497132023257	25.258593872621375
18	25.729310426878598	24.18060928268895	25.552605756527136	24.537474533905314
19	26.066341313070307	24.5942617053076	24.842242786906237	24.497154194715858
20	26.117486026113294	23.783481523196144	24.312976537014226	25.786055913676336
21	27.614639864345403	23.721878609982262	23.855244349943998	24.808237175728344
22	26.52510396247153	23.638573192979276	24.39616783565431	25.440155008894884
23	24.741233614106218	23.501409907824833	25.08627434760956	26.67108213045939
24	25.17235713395356	24.618683921266445	24.61545434972294	25.593504595057055
25	25.394656100575048	23.96431281025287	24.68503720626373	25.955993882908352
26	25.23509000167719	24.92611295199155	25.071677235989792	24.767119810341466
27	26.00954660698857	24.171385674423878	24.170535828104416	25.648531890483138
28	25.014276940927456	24.15692397642265	25.27630413056829	25.5524949520816
29	24.983597640779834	24.42518087921178	25.122465283089422	25.468756196918964
30	24.827505329644836	24.676581345138338	25.43091800193414	25.064995323282684
31	25.749341956917583	24.436881685950002	23.893564151693802	25.920212205438613
32	25.350695498860098	24.330732483354712	23.911654194975366	26.406917822809824
33	24.877948457620906	23.920119340544908	24.767695561499416	26.434236640334767
34	25.908137242848134	24.10435596520939	24.758988735715672	25.2285180562268
35	26.48116376262397	23.86644878791725	24.877046579515167	24.77534086994361
36	25.02531274770939	24.736291312755643	24.365793143149183	25.872602796385785
37	26.29359187040512	24.439209340694205	24.292524453146953	24.97467433575372
38	25.123153267534505	24.181211357918098	24.952565808743753	25.743069565803644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	104.5
2	135.0
3	135.0
4	375.5
5	616.0
6	616.0
7	738.5
8	861.0
9	818.5
10	776.0
11	776.0
12	1065.0
13	1354.0
14	1950.0
15	2546.0
16	2546.0
17	3883.5
18	5221.0
19	5221.0
20	6436.0
21	7651.0
22	8724.0
23	9797.0
24	9797.0
25	11905.5
26	14014.0
27	14014.0
28	17935.5
29	21857.0
30	27546.0
31	33235.0
32	33235.0
33	43830.0
34	54425.0
35	54425.0
36	61740.5
37	69056.0
38	80258.0
39	91460.0
40	91460.0
41	98642.0
42	105824.0
43	121457.5
44	137091.0
45	137091.0
46	140593.0
47	144095.0
48	144095.0
49	155194.5
50	166294.0
51	169435.5
52	172577.0
53	172577.0
54	165287.0
55	157997.0
56	157997.0
57	152810.5
58	147624.0
59	132574.0
60	117524.0
61	117524.0
62	112113.0
63	106702.0
64	89014.5
65	71327.0
66	71327.0
67	59737.0
68	48147.0
69	48147.0
70	38889.0
71	29631.0
72	23158.0
73	16685.0
74	16685.0
75	12786.5
76	8888.0
77	8888.0
78	8357.5
79	7827.0
80	6158.5
81	4490.0
82	4490.0
83	4590.0
84	4690.0
85	4690.0
86	3177.5
87	1665.0
88	1371.0
89	1077.0
90	1077.0
91	801.5
92	526.0
93	440.0
94	354.0
95	354.0
96	313.0
97	272.0
98	272.0
99	542.5
100	813.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12281908318500247
2	0.055971058204235445
3	0.011386824594181503
4	0.003059146905899508
5	2.2660347451107466E-4
6	4.5320694902214933E-4
7	5.6650868627768666E-5
8	6.231595549054553E-4
9	2.2660347451107466E-4
10	5.665086862776867E-4
11	0.0027192416941328964
12	0.0033424012490383516
13	0.015239083660869773
14	0.010367108958881667
15	0.02334015787464069
16	0.011953333280459189
17	0.020677567049135565
18	0.006004992074543479
19	0.008157725082398688
20	0.00679810423533224
21	0.007194660315726621
22	0.007817819870632077
23	0.01070701417064828
24	0.014615924105964318
25	0.017391816668724983
26	0.019374597070696886
27	0.009800600272603979
28	0.006458199023565629
29	0.011783380674575883
30	0.004022211672571576
31	0.006571500760821166
32	0.007591216396121002
33	0.00934739332358183
34	0.013539557602036713
35	0.01710856232558614
36	0.015692290609891923
37	0.010763665039276046
38	0.00685475510396001
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1765198.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.169487524614304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.98711554222865	45.231871480860946
2	11.10767827293787	12.256098358125502
3	3.0236147197202907	5.004338236765463
4	1.2732046452809809	2.809681911764402
5	0.6734913270530768	1.8578085682895322
6	0.41418550638461815	1.3710241276417348
7	0.27530294413763795	1.0631825639463703
8	0.19308956332547106	0.852212180401424
9	0.14112055128858392	0.7006993644404002
>10	0.7533497717117705	7.4460647163625655
>50	0.0659423166599561	2.585290213073399
>100	0.07373088427207537	9.1150722979067
>500	0.012701116277755818	4.921576401844016
>1k	0.005472838721309417	4.785079578577492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2801	0.15867908302638004	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2614	0.1480853705929873	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2442	0.13834142118901108	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2429	0.1376049598968501	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2331	0.13205317477132877	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2312	0.13097680826740118	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2181	0.12355554447716346	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2166	0.12270578144774694	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2118	0.11998653975361404	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2035	0.11528451765750924	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2006	0.11364164246730395	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1956	0.11080909903591551	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1935	0.10961943079473237	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1922	0.10888296950257138	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1872	0.10605042607118297	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1772	0.10038533920840609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.665086862776867E-5
2	0.0	0.0	0.0	0.0	5.665086862776867E-5
3	0.0	0.0	0.0	0.0	5.665086862776867E-5
4	0.0	0.0	0.0	0.0	5.665086862776867E-5
5	0.0	0.0	0.0	0.0	5.665086862776867E-5
6	0.0	0.0	0.0	0.0	1.1330173725553735E-4
7	0.0	0.0	0.0	0.0	1.1330173725553735E-4
8	0.0	0.0	0.0	0.0	1.69952605883306E-4
9	0.0	0.0	0.0	0.0	1.69952605883306E-4
10	0.0	0.0	0.0	0.0	1.69952605883306E-4
11	0.0	0.0	0.0	0.0	1.69952605883306E-4
12	0.0	0.0	0.0	0.0	3.39905211766612E-4
13	0.0	0.0	0.0	0.0	5.09857817649918E-4
14	0.0	0.0	0.0	0.0	5.665086862776867E-4
15	0.0	0.0	0.0	0.0	8.4976302941653E-4
16	0.0	0.0	0.0	0.0	8.4976302941653E-4
17	0.0	0.0	0.0	0.0	8.4976302941653E-4
18	0.0	0.0	0.0	5.665086862776867E-5	8.4976302941653E-4
19	0.0	0.0	0.0	5.665086862776867E-5	8.4976302941653E-4
20	0.0	0.0	0.0	1.1330173725553735E-4	8.4976302941653E-4
21	0.0	0.0	0.0	1.1330173725553735E-4	8.4976302941653E-4
22	0.0	0.0	0.0	1.1330173725553735E-4	8.4976302941653E-4
23	0.0	0.0	0.0	2.8325434313884333E-4	0.001019715635299836
24	0.0	0.0	0.0	4.532069490221494E-4	0.0010763665039276048
25	0.0	0.0	0.0	5.09857817649918E-4	0.0010763665039276048
26	0.0	0.0	0.0	8.4976302941653E-4	0.0011330173725553733
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	450	0.0	19.199047	28
CTAGCGG	455	0.0	18.98807	29
GTATCAA	2425	0.0	17.499533	1
GTATTAA	190	0.0	16.856552	1
AAGACGG	615	0.0	16.647223	5
CAAGACG	620	0.0	16.51344	4
TAGCGGC	545	0.0	16.440018	30
CGACCCG	425	0.0	16.185139	5
GTCCTAT	625	0.0	16.141834	1
CGTACAC	80	5.6259807E-5	15.999207	3
ATACCGT	500	0.0	15.996941	6
TACCGTC	525	0.0	15.540766	7
AAACGCT	500	0.0	15.359239	29
CTTACAC	190	1.8189894E-12	15.157143	3
AATAGCG	380	0.0	15.154995	5
GTTATTC	360	0.0	15.110362	3
AACGCTT	495	0.0	14.868372	30
GCGCAAG	705	0.0	14.764426	1
CGCAAGA	695	0.0	14.738929	2
TAAACGC	500	0.0	14.71927	28
>>END_MODULE