FastQCFastQC Report
Thu 2 Feb 2017
SRR4062841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765198
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30590.17329500713234436No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25770.14598928845375986No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25750.14587598671650434No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25150.1424769345988382No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24350.1379448651086167No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24300.13766161076547787No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23040.130523601318379No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22470.1272945018065962No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21970.12446195837520777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21960.12440530750657999No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21900.1240654022948134No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21720.12304568665951354No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21260.12043974670263619No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19150.108486413422177No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18480.1046908052241165No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18000.1019715635299836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.017.835351
ATCGTTT4950.016.80793229
CATCGTT4850.016.494728
TCTAGCG4900.016.32638528
CGTTTAT5150.016.15519731
CGGCATC5250.016.15223725
TCGTTTA5200.015.99985830
TACCGTC4900.015.6737757
TAGCGGC5300.015.09420630
CGTCGTA5750.015.02637910
GCATCGT5450.014.97234427
CGCGTAA3850.014.96133110
ATACCGT5350.014.9535626
CTAGCGG5200.014.76909929
ACCGTCG5300.014.4908498
GCGTAAC4000.014.4002811
TCGCGTA3900.014.3592549
CGCGGTT6950.014.27366110
GTTTTCG6850.014.2480528
CGCATCG5650.014.15916513
Produced by FastQC (version 0.11.5)