##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1765198
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210694211074337	32.0	32.0	32.0	32.0	32.0
2	31.235051252040847	32.0	32.0	32.0	32.0	32.0
3	31.33554366139096	32.0	32.0	32.0	32.0	32.0
4	31.438214863148495	32.0	32.0	32.0	32.0	32.0
5	31.3415157959617	32.0	32.0	32.0	32.0	32.0
6	34.872810302300365	36.0	36.0	36.0	36.0	36.0
7	34.90118898843076	36.0	36.0	36.0	36.0	36.0
8	34.82913021655361	36.0	36.0	36.0	32.0	36.0
9	34.95307495249825	36.0	36.0	36.0	32.0	36.0
10	34.78836028592826	36.0	36.0	36.0	32.0	36.0
11	34.97470708668376	36.0	36.0	36.0	36.0	36.0
12	34.86079012099493	36.0	36.0	36.0	32.0	36.0
13	34.93020046476373	36.0	36.0	36.0	36.0	36.0
14	34.86837737183024	36.0	36.0	36.0	32.0	36.0
15	34.83427354891633	36.0	36.0	36.0	32.0	36.0
16	34.85062525563704	36.0	36.0	36.0	32.0	36.0
17	34.81140812532079	36.0	36.0	36.0	32.0	36.0
18	34.81454714995145	36.0	36.0	36.0	32.0	36.0
19	34.81161830004339	36.0	36.0	36.0	32.0	36.0
20	34.79586142744327	36.0	36.0	36.0	32.0	36.0
21	34.792854399336505	36.0	36.0	36.0	32.0	36.0
22	34.77479523543534	36.0	36.0	36.0	32.0	36.0
23	34.71961672288321	36.0	36.0	36.0	32.0	36.0
24	34.68852502665423	36.0	36.0	36.0	32.0	36.0
25	34.67191442546389	36.0	36.0	36.0	32.0	36.0
26	34.6026604380925	36.0	36.0	36.0	32.0	36.0
27	34.596067976510284	36.0	36.0	36.0	32.0	36.0
28	34.56544251693011	36.0	36.0	36.0	32.0	36.0
29	34.5269210592806	36.0	36.0	36.0	32.0	36.0
30	34.50664004831186	36.0	36.0	36.0	32.0	36.0
31	34.50921199774756	36.0	36.0	36.0	32.0	36.0
32	34.48713345471726	36.0	36.0	36.0	32.0	36.0
33	34.45286307824958	36.0	36.0	36.0	32.0	36.0
34	34.45079305550992	36.0	36.0	36.0	32.0	36.0
35	34.41991946512516	36.0	36.0	36.0	32.0	36.0
36	34.388081676956354	36.0	36.0	36.0	32.0	36.0
37	34.39102638910762	36.0	36.0	36.0	32.0	36.0
38	33.94141053864779	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	38.0
22	120.0
23	411.0
24	1155.0
25	2782.0
26	6099.0
27	11889.0
28	20755.0
29	33158.0
30	50687.0
31	73265.0
32	106094.0
33	171955.0
34	417585.0
35	869197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.576178117108896	18.402575350940857	12.071107696648406	26.950138835301846
2	16.09955370445695	20.378223859306434	36.03442786588247	27.487794570354147
3	17.852558183274624	24.068234838244777	28.587161326944628	29.492045651535975
4	12.290816430583346	15.937833532366943	35.532881334424054	36.23846870262566
5	14.641085385937094	36.54345662268857	32.888680413574235	15.926777577800097
6	34.996360175504684	35.333829973459025	16.22931177575282	13.440498075283466
7	30.659283883642992	30.20817046116334	20.6162176493382	18.516328005855467
8	28.45542088678948	32.41926766729702	19.085643518922428	20.039667926991072
9	27.447475688828334	13.929246204964493	18.24421361538919	40.37906449081798
10	16.119461834835256	26.109587504525027	30.883483468852805	26.88746719178691
11	38.254448010469574	20.785727842095277	21.769459780240947	19.190364367194203
12	24.93418574151864	23.519441194882152	27.89419865793864	23.652174405660563
13	29.452293426739338	18.78447561377002	25.17866277814884	26.5845681813418
14	23.926890921018494	19.43872585398352	24.349053194032624	32.28533003096536
15	25.479804531842888	26.740059755336226	21.666124706690127	26.114011006130756
16	26.497197481528982	25.36553972982068	22.89510865070094	25.242154137949395
17	24.697909243042425	25.54274364688834	24.46394115560974	25.2954059544595
18	25.79988193958978	24.25319992431444	25.343842447136243	24.60307568895954
19	25.96615223901228	24.620977363445913	24.77693720477816	24.635933192763645
20	26.21683233269016	23.674907857362175	24.298973826165675	25.809285983781987
21	27.577189641048765	23.81166305422961	23.755975250368515	24.85517205435311
22	26.37092269535769	23.736940558509584	24.456179986607733	25.43595675952499
23	24.68691826507455	23.45504233529006	25.117862399260364	26.74017700037503
24	25.207087250268806	24.638822387063662	24.5624003652848	25.591689997382726
25	25.3193836155398	23.90169482499687	24.740014853866167	26.038906705597164
26	25.190998403578526	24.900719352729837	25.039457329999244	24.868824913692404
27	26.088195455114537	24.032499562370802	24.112717419568284	25.766587562946373
28	24.964904786885096	24.119730477827417	25.327583647840076	25.587781087447414
29	24.93731581386337	24.325713036157985	25.179441626378456	25.557529523600188
30	24.935219731724146	24.56347673178873	25.365709682426562	25.135593854060563
31	25.695587690446057	24.372110097564125	24.02642649719748	25.905875714792337
32	25.458957012187867	24.20312055644749	23.998724222438504	26.339198208926142
33	24.927288610116257	23.817384791961015	24.855511959564875	26.39981463835785
34	25.830983266466422	23.971928361577568	24.889615782478796	25.307472589477214
35	26.398851573591177	23.857323654343592	24.85437894219232	24.88944582987291
36	25.052883585864024	24.56392993873775	24.469606242472516	25.91358023292571
37	26.302205191712204	24.39029502639364	24.275180461342014	25.032319320552144
38	25.162984733706622	23.966063825206945	24.93575784218863	25.935193598897797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	54.5
2	74.0
3	74.0
4	296.0
5	518.0
6	518.0
7	613.5
8	709.0
9	724.0
10	739.0
11	739.0
12	1005.5
13	1272.0
14	1883.0
15	2494.0
16	2494.0
17	3884.0
18	5274.0
19	5274.0
20	6511.0
21	7748.0
22	8773.0
23	9798.0
24	9798.0
25	12032.5
26	14267.0
27	14267.0
28	18273.5
29	22280.0
30	27777.0
31	33274.0
32	33274.0
33	44279.5
34	55285.0
35	55285.0
36	62368.0
37	69451.0
38	80526.0
39	91601.0
40	91601.0
41	98755.5
42	105910.0
43	121719.5
44	137529.0
45	137529.0
46	140553.0
47	143577.0
48	143577.0
49	155231.0
50	166885.0
51	169455.0
52	172025.0
53	172025.0
54	164755.5
55	157486.0
56	157486.0
57	153171.0
58	148856.0
59	133551.5
60	118247.0
61	118247.0
62	112504.5
63	106762.0
64	88930.0
65	71098.0
66	71098.0
67	60086.5
68	49075.0
69	49075.0
70	39406.0
71	29737.0
72	23122.0
73	16507.0
74	16507.0
75	12458.5
76	8410.0
77	8410.0
78	8118.5
79	7827.0
80	6115.5
81	4404.0
82	4404.0
83	4205.0
84	4006.0
85	4006.0
86	2552.0
87	1098.0
88	883.5
89	669.0
90	669.0
91	418.0
92	167.0
93	110.5
94	54.0
95	54.0
96	37.5
97	21.0
98	21.0
99	25.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008894186374559682
2	0.0
3	0.0
4	1.1330173725553733E-4
5	5.6650868627768666E-5
6	1.6995260588330603E-4
7	5.665086862776867E-4
8	0.0024926382196218213
9	0.0035690047235494263
10	8.4976302941653E-4
11	0.0047020220961048
12	1.6995260588330603E-4
13	5.6650868627768666E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.2660347451107466E-4
24	0.0
25	5.6650868627768666E-5
26	0.0
27	2.8325434313884333E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.1330173725553733E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1765198.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.46902885642727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83200657660143	43.220088921672215
2	11.794162002623821	12.612447769113425
3	3.253537822745254	5.218905231895305
4	1.3630507124482354	2.915239915066738
5	0.6991668910315364	1.8691887336011883
6	0.4347926207835708	1.394876351234303
7	0.29479202738526966	1.1033570393235377
8	0.20670572281272967	0.884188340629
9	0.14770222006611608	0.710774484018618
>10	0.8080494327049697	7.7299819843853355
>50	0.06743765494060268	2.5441636710468094
>100	0.07883473724591063	9.374051166428275
>500	0.012925140604740497	4.812646525365012
>1k	0.006836438005813156	5.610089866220275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3059	0.17329500713234436	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2577	0.14598928845375986	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2575	0.14587598671650434	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2515	0.1424769345988382	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2435	0.1379448651086167	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2430	0.13766161076547787	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2304	0.130523601318379	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2247	0.1272945018065962	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2197	0.12446195837520777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2196	0.12440530750657999	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2190	0.1240654022948134	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2172	0.12304568665951354	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2126	0.12043974670263619	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1915	0.108486413422177	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1848	0.1046908052241165	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1800	0.1019715635299836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1330173725553735E-4
2	0.0	0.0	0.0	0.0	1.1330173725553735E-4
3	0.0	0.0	0.0	0.0	1.1330173725553735E-4
4	0.0	0.0	0.0	0.0	1.1330173725553735E-4
5	0.0	0.0	0.0	0.0	1.1330173725553735E-4
6	0.0	0.0	0.0	0.0	1.1330173725553735E-4
7	0.0	0.0	0.0	0.0	1.1330173725553735E-4
8	0.0	0.0	0.0	0.0	1.1330173725553735E-4
9	0.0	0.0	0.0	0.0	1.1330173725553735E-4
10	0.0	0.0	0.0	0.0	1.1330173725553735E-4
11	0.0	0.0	0.0	0.0	1.1330173725553735E-4
12	0.0	0.0	0.0	0.0	3.39905211766612E-4
13	0.0	0.0	0.0	0.0	3.9655608039438066E-4
14	0.0	0.0	0.0	0.0	3.9655608039438066E-4
15	0.0	0.0	0.0	0.0	5.09857817649918E-4
16	0.0	0.0	0.0	0.0	5.665086862776867E-4
17	0.0	0.0	0.0	0.0	5.665086862776867E-4
18	0.0	0.0	0.0	0.0	5.665086862776867E-4
19	0.0	0.0	0.0	0.0	5.665086862776867E-4
20	0.0	0.0	0.0	5.665086862776867E-5	6.79810423533224E-4
21	0.0	0.0	0.0	5.665086862776867E-5	9.064138980442988E-4
22	0.0	0.0	0.0	5.665086862776867E-5	9.064138980442988E-4
23	0.0	0.0	0.0	1.69952605883306E-4	9.630647666720674E-4
24	0.0	0.0	0.0	3.9655608039438066E-4	9.630647666720674E-4
25	0.0	0.0	0.0	4.532069490221494E-4	9.630647666720674E-4
26	0.0	0.0	0.0	7.364612921609927E-4	0.0010763665039276048
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2530	0.0	17.83535	1
ATCGTTT	495	0.0	16.807932	29
CATCGTT	485	0.0	16.4947	28
TCTAGCG	490	0.0	16.326385	28
CGTTTAT	515	0.0	16.155197	31
CGGCATC	525	0.0	16.152237	25
TCGTTTA	520	0.0	15.999858	30
TACCGTC	490	0.0	15.673775	7
TAGCGGC	530	0.0	15.094206	30
CGTCGTA	575	0.0	15.026379	10
GCATCGT	545	0.0	14.972344	27
CGCGTAA	385	0.0	14.961331	10
ATACCGT	535	0.0	14.953562	6
CTAGCGG	520	0.0	14.769099	29
ACCGTCG	530	0.0	14.490849	8
GCGTAAC	400	0.0	14.40028	11
TCGCGTA	390	0.0	14.359254	9
CGCGGTT	695	0.0	14.273661	10
GTTTTCG	685	0.0	14.24805	28
CGCATCG	565	0.0	14.159165	13
>>END_MODULE