FastQCFastQC Report
Thu 2 Feb 2017
SRR4062840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126212
Sequences flagged as poor quality0
Sequence length38
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34322.7192343041866067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24071.907108674294045No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20491.6234589420974235No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16281.289893195575698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13591.0767597375843818No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12080.9571197667416728No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.8176718537064621No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7300.578391912021044No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6980.5530377460146421No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6130.4856907425601369No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5860.4642981649922353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5730.45399803505213454No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5710.4524133996767344No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5210.4127975152917314No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.3502044179634266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4070.3224732988939245No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4040.3200963458308243No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.316134757392324No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3980.315342439704624No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC3770.2987037682629227No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3600.28523436757202164No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC3510.27810350838272113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.26780337844262037No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA3110.24641080087471876No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG2890.2289798117453174No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2790.2210566348683168No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2540.20124869267581527No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2530.20045637498811525No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2380.18857160967261433No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA2280.1806484327956137No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2270.1798561151079137No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT2180.17272525591861312No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2170.17193293823091307No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2140.16955598516781287No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC2030.16084049060311223No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC1990.157671219852312No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1930.15291731372611164No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC1900.15054036066301144No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA1890.14974804297531139No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1840.14578645453681108No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1830.14499413684911103No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG1790.1418248660983108No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA1770.14024023072291067No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1690.13390168922131018No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA1610.1275631477197097No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1550.12280924159350932No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA1540.12201692390580926No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG1510.1196399708427091No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1500.11884765315500903No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA1450.11488606471650874No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1390.11013215859030838No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA1370.10854752321490825No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1350.10696288783950812No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA1340.10617057015180806No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1330.105378252464108No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT1320.10458593477640796No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1310.10379361708870789No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1290.10220898171330776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAATG308.376426E-426.66600829
TAGGACC605.8862497E-926.6660084
ATAGGAC350.00205672322.8565773
AGTGACT350.00205672322.85657723
TATGTGT350.00205672322.85657725
TGACTAA350.00205672322.85657725
GTGTTTT653.7620157E-722.15329728
CTAATAC453.5133213E-421.3328043
ATGTGTT707.673916E-720.57091926
TGTGTTT707.673916E-720.57091927
AAAGTTT400.004462421419.99950430
TGCCTGC400.004462421419.99950414
CAGTGAC400.004462421419.99950422
GTGACTA400.004462421419.99950424
GCACTGC400.004462421419.99950410
GACTAAA400.004462421419.99950426
ACCATTG507.148686E-419.1995248
CCACCTT852.3548455E-718.82306328
GTCCTAC1850.018.1689111
AAGTACT802.757044E-617.9995544
Produced by FastQC (version 0.11.5)