FastQCFastQC Report
Thu 2 Feb 2017
SRR4062840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126212
Sequences flagged as poor quality0
Sequence length38
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43043.4101353278610596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32592.5821633442144964No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21501.7034830285551295No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC20471.6218743067220234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19931.5790891515862202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13701.0854752321490826No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10180.8065794060786613No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7980.632269514784648No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6280.4975755078756378No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA6030.47776756568313633No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5800.45954425886603495No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.45954425886603495No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5630.4460748581751339No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.4365670459227332No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5340.4230976452318322No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.41992837448103193No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4960.3929895730992299No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC4940.3914049377238297No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4810.38110480778372896No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4670.3700123601559281No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4250.3367350172725256No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3970.3145501220169239No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3750.29711913288752256No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA3560.28206509682122144No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3100.24561848318701865No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2930.23214908249611765No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2850.2258105409945172No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT2810.22264127024371694No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC2770.21947199949291668No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2520.19966405730041514No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2430.19253319811111463No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2420.19174088042341456No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2350.18619465660951415No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA2270.1798561151079137No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2130.16876366748011284No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC2110.16717903210471272No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2020.16004817291541215No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2020.16004817291541215No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT2010.1592558552277121No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC2000.15846353754001205No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1940.15370963141381166No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1910.1513326783507115No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1910.1513326783507115No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA1860.1473710899122112No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA1850.14657877222451116No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1730.13707095997211044No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA1640.12994010078280988No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG1620.12835546540740975No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA1600.12677083003200962No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG1600.12677083003200962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.12439387696890945No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA1550.12280924159350932No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA1530.12122460621810921No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG1520.12043228853040916No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1490.11805533546730897No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1490.11805533546730897No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1480.1172630177796089No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA1460.11567838240420877No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT1430.11330142934110861No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT1380.10933984090260832No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1380.10933984090260832No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA1370.10854752321490825No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA1360.10775520552720819No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1360.10775520552720819No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1340.10617057015180806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1290.10220898171330776No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1290.10220898171330776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1280.1014166640256077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAA200.003740970732.012
CGTGCAA200.003740970732.011
CCGTGCA200.003740970732.010
AGAGTGT200.003740970732.06
TGACCGT200.003740970732.07
ACCGTGC200.003740970732.09
CTGTGCA200.003740970732.09
TATCCTG200.003740970732.02
TGCAAAG200.003740970732.013
ATTTAGA453.512734E-421.3333341
TATACAC453.512734E-421.3333343
AGAACAG555.7807727E-520.3636365
ATATACA555.7807727E-520.3636362
ACTGTTC400.00446178220.08
TAGGACC659.3252E-619.6923084
GGACCTG901.9956133E-819.5555556
TGTGCAT1500.019.210
ATACACT507.1474933E-419.24
AGTGTGC1450.018.7586218
TTGTAGA601.1280186E-418.6666681
Produced by FastQC (version 0.11.5)