##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062840_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126212
Sequences flagged as poor quality	0
Sequence length	38
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.358690140398693	32.0	32.0	32.0	21.0	32.0
2	31.293442778816594	32.0	32.0	32.0	32.0	32.0
3	31.38216651348525	32.0	32.0	32.0	32.0	32.0
4	31.538268944315913	32.0	32.0	32.0	32.0	32.0
5	31.370495673945424	32.0	32.0	32.0	32.0	32.0
6	34.8258564954204	36.0	36.0	36.0	32.0	36.0
7	34.910705796596204	36.0	36.0	36.0	36.0	36.0
8	34.92340664913004	36.0	36.0	36.0	36.0	36.0
9	34.93395239755332	36.0	36.0	36.0	32.0	36.0
10	34.847613539124644	36.0	36.0	36.0	32.0	36.0
11	34.993328685069564	36.0	36.0	36.0	36.0	36.0
12	34.90619750895319	36.0	36.0	36.0	32.0	36.0
13	34.90250530852851	36.0	36.0	36.0	36.0	36.0
14	34.85819098025544	36.0	36.0	36.0	32.0	36.0
15	34.828645453681105	36.0	36.0	36.0	32.0	36.0
16	34.80737172376636	36.0	36.0	36.0	32.0	36.0
17	34.795447342566476	36.0	36.0	36.0	32.0	36.0
18	34.72319589262511	36.0	36.0	36.0	32.0	36.0
19	34.65880423414572	36.0	36.0	36.0	32.0	36.0
20	34.58540392355719	36.0	36.0	36.0	32.0	36.0
21	34.49422400405667	36.0	36.0	36.0	32.0	36.0
22	34.503708046778435	36.0	36.0	36.0	32.0	36.0
23	34.44080594555193	36.0	36.0	36.0	32.0	36.0
24	34.43520425949989	36.0	36.0	36.0	32.0	36.0
25	34.35855544639178	36.0	36.0	36.0	32.0	36.0
26	34.30322790225969	36.0	36.0	36.0	32.0	36.0
27	34.25190156245048	36.0	36.0	36.0	32.0	36.0
28	34.16583209203562	36.0	36.0	36.0	32.0	36.0
29	34.09506227617025	36.0	36.0	36.0	32.0	36.0
30	33.996925807371724	36.0	36.0	36.0	32.0	36.0
31	33.93171806167401	36.0	36.0	36.0	32.0	36.0
32	33.798798846385445	36.0	36.0	36.0	32.0	36.0
33	33.656577821443285	36.0	36.0	36.0	27.0	36.0
34	33.45508351028428	36.0	36.0	36.0	21.0	36.0
35	33.26349317022153	36.0	36.0	36.0	14.0	36.0
36	33.149027033879506	36.0	36.0	36.0	14.0	36.0
37	33.02777073495389	36.0	36.0	36.0	14.0	36.0
38	32.62349855798181	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	7.0
22	15.0
23	44.0
24	108.0
25	287.0
26	620.0
27	1280.0
28	2328.0
29	3537.0
30	5242.0
31	7240.0
32	9672.0
33	13758.0
34	27146.0
35	54924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.428766124687016	18.349656112326077	14.940096985832463	19.281480777154446
2	11.795233416790797	24.424777358729756	41.47703863341045	22.302950591068996
3	18.419009285963302	33.76541057902577	29.17630653186702	18.639273603143916
4	9.98716445345926	19.297689601622668	35.16860520394257	35.5465407409755
5	9.049060311222387	42.561721547871834	32.34953887110576	16.03967926980002
6	26.247108040439894	37.82920799923937	20.005229296738822	15.91845466358191
7	23.554206844094413	35.542068440944135	22.327689345619635	18.576035369341817
8	29.325166984921836	31.54767092679603	20.713261336354776	18.413900751927358
9	29.303678113560373	14.197423260383818	20.108394212636483	36.39050441341933
10	17.17441427450856	29.53942207889962	31.17874036335977	22.10742328323205
11	31.866442697768765	24.507162778904664	22.708544624746448	20.917849898580123
12	25.227395176369917	28.43548949386746	30.53830063702342	15.798814692739201
13	32.681519982252084	23.485088581117488	27.128165309162362	16.70522612746807
14	24.778151047443984	22.063670649383578	30.212658067378694	22.945520235793744
15	23.897886096409216	27.923652267613225	32.77659810477609	15.401863531201471
16	19.02909390549235	27.709726491934205	33.065794060786615	20.195385541786834
17	17.49199759135423	27.00456374988115	33.301112414033526	22.20232624473109
18	19.30244350774887	22.87738091465154	39.98114283903274	17.839032738566857
19	20.28967134662314	27.27870566982537	35.75175102208982	16.679871961461668
20	22.297404367255094	21.84261401451526	39.95737330840174	15.90260830982791
21	23.320286502075874	23.7116914397997	32.412924286121765	20.55509777200266
22	20.83320128038538	26.59176623458942	33.68697112794346	18.888061357081735
23	18.529933762241306	26.899185497417044	34.66310651918994	19.907774221151715
24	18.636104332393117	28.81580198396349	35.16147434475327	17.38661933889012
25	21.42347795772193	25.228979811745315	34.563274490539726	18.784267739993027
26	16.57132443824676	25.980096979684973	37.74046841821696	19.7081101638513
27	20.066396748302445	28.065699503212876	35.92396859227801	15.94393515620667
28	17.90558742433366	26.05774411307958	36.19307197413875	19.84359648844801
29	17.24558679047951	27.299305929705575	37.42908756695085	18.02601971286407
30	19.06870978987735	26.153614553291288	37.28330111241404	17.49437454441733
31	17.415142775647325	26.967324818559252	35.5869489430482	20.03058346274522
32	15.660159097391691	29.89731562767407	35.5996260260514	18.842899248882834
33	15.842392165562705	29.31416980952683	35.826228884733624	19.017209140176845
34	15.7742528444205	29.932177605932875	37.39501790637974	16.898551643266885
35	18.54498779830761	29.62634297848065	35.28507590403448	16.54359331917726
36	17.566475453998034	30.321997908281308	35.13770481412227	16.97382182359839
37	18.64165055620702	30.92099008018255	33.57446201629005	16.862897347320384
38	17.549836782556334	32.342408011916454	32.120559059360446	17.987196146166767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	298.0
1	238.0
2	178.0
3	178.0
4	595.5
5	1013.0
6	1013.0
7	1245.5
8	1478.0
9	1440.5
10	1403.0
11	1403.0
12	1735.5
13	2068.0
14	2326.0
15	2584.0
16	2584.0
17	3835.5
18	5087.0
19	5087.0
20	5261.0
21	5435.0
22	4416.5
23	3398.0
24	3398.0
25	2897.5
26	2397.0
27	2397.0
28	3283.5
29	4170.0
30	5284.0
31	6398.0
32	6398.0
33	6508.5
34	6619.0
35	6619.0
36	6594.0
37	6569.0
38	7010.0
39	7451.0
40	7451.0
41	7659.5
42	7868.0
43	8474.0
44	9080.0
45	9080.0
46	13318.0
47	17556.0
48	17556.0
49	15117.5
50	12679.0
51	9255.0
52	5831.0
53	5831.0
54	4867.5
55	3904.0
56	3904.0
57	3700.5
58	3497.0
59	3109.0
60	2721.0
61	2721.0
62	2397.5
63	2074.0
64	1801.0
65	1528.0
66	1528.0
67	1316.0
68	1104.0
69	1104.0
70	945.5
71	787.0
72	638.0
73	489.0
74	489.0
75	372.5
76	256.0
77	256.0
78	202.0
79	148.0
80	103.5
81	59.0
82	59.0
83	52.5
84	46.0
85	46.0
86	34.0
87	22.0
88	14.5
89	7.0
90	7.0
91	4.5
92	2.0
93	4.0
94	6.0
95	6.0
96	4.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006338541501600481
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	7.923176877000602E-4
8	0.0023769530631001807
9	0.004753906126200361
10	7.923176877000602E-4
11	0.0031692707508002406
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	7.923176877000602E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	126212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.16664025607708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.39633443246414	29.921877475992776
2	12.720247607873283	9.964187240515958
3	4.193554912710133	4.927423699806675
4	1.8489672890579167	2.8967134662314202
5	1.0539518135658366	2.063987576458657
6	0.6615014261727996	1.5545273032675182
7	0.4693221127586834	1.2867239248248978
8	0.32973924301579915	1.0331822647608786
9	0.2751198592033661	0.9697968497448737
>10	1.7053385390326299	12.950432605457484
>50	0.18004167256690876	4.895730992298672
>100	0.13351404931928063	10.494247773587299
>500	0.018206461270811	4.252369029886223
>1k	0.014160580988408552	12.788799797166671
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4304	3.4101353278610596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3259	2.5821633442144964	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2150	1.7034830285551295	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2047	1.6218743067220234	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1993	1.5790891515862202	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1370	1.0854752321490826	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1018	0.8065794060786613	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	798	0.632269514784648	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	628	0.4975755078756378	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	603	0.47776756568313633	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	580	0.45954425886603495	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	580	0.45954425886603495	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	563	0.4460748581751339	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.4365670459227332	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	534	0.4230976452318322	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.41992837448103193	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	496	0.3929895730992299	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	494	0.3914049377238297	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	481	0.38110480778372896	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	467	0.3700123601559281	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	425	0.3367350172725256	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	397	0.3145501220169239	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	375	0.29711913288752256	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	356	0.28206509682122144	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	310	0.24561848318701865	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	293	0.23214908249611765	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	285	0.2258105409945172	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	281	0.22264127024371694	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	277	0.21947199949291668	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	252	0.19966405730041514	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.19253319811111463	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	242	0.19174088042341456	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	235	0.18619465660951415	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	227	0.1798561151079137	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	213	0.16876366748011284	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	211	0.16717903210471272	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	202	0.16004817291541215	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	202	0.16004817291541215	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	201	0.1592558552277121	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	200	0.15846353754001205	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	194	0.15370963141381166	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	191	0.1513326783507115	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	191	0.1513326783507115	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	186	0.1473710899122112	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	185	0.14657877222451116	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	173	0.13707095997211044	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	164	0.12994010078280988	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	162	0.12835546540740975	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	160	0.12677083003200962	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	160	0.12677083003200962	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	157	0.12439387696890945	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	155	0.12280924159350932	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	153	0.12122460621810921	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	152	0.12043228853040916	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	149	0.11805533546730897	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	149	0.11805533546730897	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	148	0.1172630177796089	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	146	0.11567838240420877	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	143	0.11330142934110861	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	138	0.10933984090260832	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	138	0.10933984090260832	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	137	0.10854752321490825	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	136	0.10775520552720819	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	136	0.10775520552720819	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	134	0.10617057015180806	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	129	0.10220898171330776	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	129	0.10220898171330776	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	128	0.1014166640256077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	7.923176877000603E-4	0.0
17	0.0	0.0	0.0	7.923176877000603E-4	0.0
18	0.0	0.0	0.0	7.923176877000603E-4	0.0
19	0.0	0.0	0.0	7.923176877000603E-4	0.0
20	0.0	0.0	0.0	0.0015846353754001205	0.0
21	0.0	0.0	0.0	0.0023769530631001807	0.0
22	0.0	0.0	0.0	0.004753906126200361	0.0
23	0.0	0.0	0.0	0.012677083003200964	0.0
24	0.0	0.0	0.0	0.027731119069502107	0.0
25	0.0	0.0	0.0	0.03961588438500301	0.0
26	0.0	0.0	0.0	0.051500649700503914	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAA	20	0.0037409707	32.0	12
CGTGCAA	20	0.0037409707	32.0	11
CCGTGCA	20	0.0037409707	32.0	10
AGAGTGT	20	0.0037409707	32.0	6
TGACCGT	20	0.0037409707	32.0	7
ACCGTGC	20	0.0037409707	32.0	9
CTGTGCA	20	0.0037409707	32.0	9
TATCCTG	20	0.0037409707	32.0	2
TGCAAAG	20	0.0037409707	32.0	13
ATTTAGA	45	3.512734E-4	21.333334	1
TATACAC	45	3.512734E-4	21.333334	3
AGAACAG	55	5.7807727E-5	20.363636	5
ATATACA	55	5.7807727E-5	20.363636	2
ACTGTTC	40	0.004461782	20.0	8
TAGGACC	65	9.3252E-6	19.692308	4
GGACCTG	90	1.9956133E-8	19.555555	6
TGTGCAT	150	0.0	19.2	10
ATACACT	50	7.1474933E-4	19.2	4
AGTGTGC	145	0.0	18.758621	8
TTGTAGA	60	1.1280186E-4	18.666668	1
>>END_MODULE